ABSTRACT
The scheme of the complex microbiological diagnostics of cholera, dysentery, salmonellosis and escherichiosis has been developed. The scheme includes the introduction of material taken from a patient with acute enteric infection into 1% peptone water (pH 8.0), followed by inoculation into Endo medium (pH 7.4-8.0) and the selection of colonies on MC-agar with the subsequent final identification of bacteria with the use of biochemical, serological and genetic methods of investigation. This scheme is proposed for use in bacteriological sections of specialized antiepidemic brigades working on the liquidation of the consequences of emergency situations when the epidemiological safety of the population is threatened.
Subject(s)
Bacteria/isolation & purification , Bacterial Infections/microbiology , Disasters , Gastrointestinal Diseases/microbiology , Agar , Bacterial Infections/diagnosis , Bacteriological Techniques , Cholera/microbiology , Culture Media , Disease Outbreaks/prevention & control , Emergency Medical Services , Enterobacteriaceae Infections/microbiology , Escherichia coli/isolation & purification , Gastrointestinal Diseases/diagnosis , Guidelines as Topic , Humans , Hydrogen-Ion Concentration , Peptones , Salmonella/isolation & purification , Shigella/isolation & purification , Vibrio cholerae/isolation & purificationABSTRACT
The results of complete identification revealed the phenotypical similarity of V. cholerae strains isolated from surface water reservoirs im Grozny in 1995 and 2000. The strains under study proved to be atoxigenic, hemolysis-positive and phage resistant. The modified method of subculturing from peptone water 1 to peptone water 2 was more labor-consuming, but it was used in the bacteriological laboratory of the specialized antiepidemic brigade with the aim of more profound study of open reservoirs for the presence of V. cholerae with the positive result.
Subject(s)
Vibrio cholerae O1/classification , Water Microbiology , Bacterial Proteins , Bacteriological Techniques/methods , Culture Media , Hemolysin Proteins , Peptones , Phenotype , Russia , Species Specificity , Vibrio cholerae O1/immunology , Vibrio cholerae O1/isolation & purification , WarfareABSTRACT
Vibrio cholerae eltor has been shown to exist in two variants: epidemic (V. cholerae eltor Hly-, tox+) and nonepidemic, or endemic (V. cholerae eltor Hly+, tox-); each of these variants determines the corresponding form of manifestation of the infection among the population and requires a differentiated complex of antiepidemic measures, as well as different tactics for the treatment of patients.
