ABSTRACT
Antiviral activity of the polyphenol complex from seagrass of the Zosteraceae family against highly pathogenic strain of the tick-borne encephalitis virus was studied on passaged culture of porcine embryo kidney cells. The antiviral effect of the test compound manifested in a decrease in the infectious titer of the virus and depended on the concentration and application schemes. Polyphenol complex in a concentration of 100 µg/ml suppressed accumulation of the pathogen in the cell culture: pretreatment of the virus reduced its titer by 4 log, pretreatment of cells by 1.4 log, and application of the compound 1 h after cell infection by 2.8 log. Antiviral action of the test compound is determined by direct inactivation of the virus and inhibition of virus replication at the early stage, which attests to potential of this compound in the treatment of tick-borne encephalitis.
Subject(s)
Antiviral Agents/pharmacology , Encephalitis Viruses, Tick-Borne/drug effects , Polyphenols/pharmacology , Zosteraceae/chemistry , Animals , Antiviral Agents/chemistry , Encephalitis Viruses, Tick-Borne/pathogenicity , Polyphenols/chemistry , Swine , Virus Replication/drug effectsABSTRACT
The review considers recent experimental studies of biological activity and mechanisms of therapeutic action of rosmarinic acid, luteolin and its sulfated derivatives in diseases associated with disorders of carbohydrate and lipid metabolism. Particular attention is focused on the results of studies showing a high therapeutic potential of these phenolic compounds in their prophylactic and therapeutic use at experimental modeling of type 2 diabetes and hyperlipidemia. Based on the analysis of our results and the literature data putative mechanisms of therapeutic action of rosmarinic acid, luteolin and its sulfated derivatives have been proposed.
Subject(s)
Cinnamates , Depsides , Diabetes Mellitus, Type 2 , Hyperlipidemias , Luteolin , Animals , Cinnamates/pharmacokinetics , Cinnamates/therapeutic use , Depsides/pharmacokinetics , Depsides/therapeutic use , Diabetes Mellitus, Type 2/drug therapy , Diabetes Mellitus, Type 2/metabolism , Disease Models, Animal , Humans , Hyperlipidemias/drug therapy , Hyperlipidemias/metabolism , Luteolin/metabolism , Luteolin/pharmacokinetics , Luteolin/therapeutic use , Rosmarinic AcidABSTRACT
Two enzymes possessing collagenolytic activity were isolated from the hepatopancreas of crab Paralithodes camtschatica by ammonium sulfate fractionation and DEAE-Sepharose chromatography. It was shown that the specific activities of proteases A and C toward insoluble collagen were equal to 400 and 300 Mandl units/mg protein, respectively. The mol. wt of homogenous proteases A and C determined by gradient polyacrylamide gel electrophoresis in the presence of SDS and 2-mercaptoethanol were equal to 30 and 24 kDa, respectively. The isoelectric point values for the enzymes were determined as 2.5 and 2.9. Both enzymes lack carbohydrates. The amino acid compositions of two crab proteases were measured. The optimal conditions for the enzyme catalysis and the catalytic constants for collagenolytic proteases A and C with respect to Bz-Arg-pNA and Bz-Tyr-OEt have been determined. Inhibition data led to classification of the purified enzymes as serine proteases.
