Quantifying microplastics in fishes: The first case study contrasting the perspective of untrained and experienced researchers.

Microplastic studies hold a low explored potential for citizen science and environmental education, but methodological issues challenge data produced by non-specialists. We compared microplastic abundance and diversity in the red tilapia Oreochromis niloticus recovered by untrained students with those recovered by researchers that have experience of three years studying the incorporation of this pollutant by aquatic organisms. Seven students dissected 80 specimens and performed digestion of digestive tract in hydrogen peroxide. The solution was filtered and inspected under a stereomicroscope by the students and by two expert researchers. A control treatment consisted of 80 samples handled only by experts. The students overestimated the abundance of fibers and fragments. Striking differences in abundance and richness of microplastics were verified between the fish dissected by students and by expert researchers. Therefore, citizen science projects involving the uptake of microplastics by fish should provide training until a satisfactory level of expertise is reached.

Mortality of the Atlantic ghost crab Ocypode quadrata (Fabricius, 1787) due to vehicle traffic on sandy beaches: A road ecology approach.

Sandy beaches are not roads, but they have been used as such worldwide, threatening endemic fauna such as ghost crabs (Crustacea: Ocypodidae). The objective of the present study was to identify the spatial factors influencing the incidence of ghost crabs (Ocypode quadrata) killed by vehicles. This study included a systematic study of carcasses with clear signals of crushing by cars on beaches with distinct urbanization levels and on dirt roads crossing low-urbanized beach stretches. Predictive variables (e.g., tyre tracks on the sand, proxies of urbanization, distance from coastal lagoons and beach width) were obtained for the kill points and random points. Generalized linear models with binomial distributions showed that the number of tyre tracks nearby (positive correlation) and indicators of urbanization in the environment (negative correlation) were the main variables explaining ghost crab kills on the beach. Similarly, the likelihood of finding crabs killed by vehicles on the dirt road was associated with the areas with the densest ghost crab populations (higher beach width and low-urbanized areas). Therefore, as an important conservation strategy and mitigation action, vehicle traffic must be severely controlled mainly on low-urbanized beaches, both on the sand and dirt roads crossing natural beach vegetation.

Can the Atlantic ghost crab be a potential biomonitor of microplastic pollution of sandy beaches sediment?

The objective of the present study was to test whether the Atlantic ghost crab Ocypode quadrata is a reliable biomonitor of microplastic (MP) pollution of beach sediments. To test the hypothesis (H1) that the sediment is the main source of MP ingestion, the proportion of MP types (hard plastic, microfibers, pellet, soft plastic, and extruded polystyrene foam) in the gut content was compared with that on the strandline. The types of MPs in the gut content and sediment had similar proportions; black (~49%) and blue (~45%) microfibers were responsible for this similarity (55%), hence confirming H1. However, the second hypothesis (H2) that prevalence of MP in the gut content is related to its density on beach with distinct urbanization degree was not accepted. These results indicate that high trophic plasticity of the ghost crab and, consequently, multiple-sources of contamination may interfere with its use as a biomonitor of MP pollution.