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J Biomol Struct Dyn ; 5(1): 105-17, 1987 Aug.
Article in English | MEDLINE | ID: mdl-3271460

ABSTRACT

The assembly origin (AO) region of the tobacco mosaic virus RNA melts in an usually narrow (2.5 degrees C) temperature range. In an 0.01 M phosphate buffer the melting temperature of AO was found to be 41.5 degrees C. This value corresponds to the regions with the most stable secondary/tertiary structure of the whole TMV RNA molecule. It is assumed that the AO region has a specific tertiary structure, which is maintained by the long-range interactions as well as by interactions of the pseudoknot type.


Subject(s)
RNA, Viral/ultrastructure , Regulatory Sequences, Nucleic Acid , Base Sequence , Genes, Viral , Molecular Sequence Data , Nucleic Acid Conformation/drug effects , Nucleic Acid Denaturation/drug effects , Osmolar Concentration , RNA, Viral/drug effects , Sodium Chloride/pharmacology , Temperature , Tobacco Mosaic Virus/genetics
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