Electrospinning cellulose acetate/silk fibroin/Au-Ag hybrid composite nanofiber for enhanced biocidal activity against MCF-7 breast cancer cell.

The development of multifunctional nanomaterials with enhanced biocompatible potential is crucial for effective biomedical applications. Herein we propose electrospun silk fibroin/cellulose acetate/gold-silver nanoparticles (CA/SF/Au-Ag) composite nanofiber for anticancer applications. The silk fibroin and cellulose acetate serving as the reducing and stabilizing agent for Ag+ and Au+ ions with improved biocompatibility. The fabricated CA/SF/Au-Ag nanofiber was studied with different functional, surface and crystallographic techniques. The CA/SF polymer matrix was formed in the needle and rod-shaped morphology with the range of 86.02 ± 57.35 nm in diameter and the Au and Ag NPs were embedded on the fiber matrix with an average size of 17.32 nm and 53.21 nm respectively. Further, it strongly triggers the cytotoxic effects against MCF-7 and MDA-MB-231 human breast cancer cells with an effective IC50 value. Our findings implied that CA/SF/Au-Ag composite nanofibers are an effective material for safer anticancer applications.

Ionic liquid - A greener templating agent with Justicia adhatoda plant extract assisted green synthesis of morphologically improved Ag-Au/ZnO nanostructure and it's antibacterial and anticancer activities.

Metal and metal oxide nanoparticles (NPs) possess significant properties that are promising materials for biological applications. In this research work, we prepared ionic liquid assisted Ag-Au/ZnO NPs, using J.adhatoda leaves extract by hydrothermal method. Ionic liquids performed as a stabilizing and templating agent to improve the surface morphology of the synthesized Ag and Au doped ZnO NPs. The prepared ZnO, Ag-doped ZnO, Au doped ZnO, and AgAu doped ZnO NPs exhibit the average crystalline size of 36, 34, 32, and 25 nm and their band gap energy values are 3.36, 3.29, 3.17, and 2.98 eV respectively. The XRD and UV-DRS result shows that after doping of Au and Ag the ZnO crystalline size and band gap energy was decreased, which leads to enhanced the biomedical (antibacterial and anticancer) properties of AgAu doped ZnO NPs. The Raman active mode of A1 (LO) and A1 (TO) showed that the formation of lattice defects due to the Ag and Au doping in the ZnO crystalline plane to improve the Ag-Au/ZnO properties. SEM and TEM images revealed that the prepared AgAu doped ZnO NPs exhibits nano stick shape with particle size range from 20 to 25 nm. The EDX spectrum and elemental mapping results confirmed that Ag and Au atoms are doped and spread over the ZnO NPs. The corresponding SAED pattern also confirms the crystallinity of Ag-Au/ZnO NPs. Furthermore, the synthesized Ag-Au/ZnO NPs has been explored for its antibacterial and anticancer activities. It shows good antibacterial activity against E.coli and S.aureus bacteria. Additionally, the Ag-Au/ZnO NPs show excellent anticancer activity against the HeLa cancer cells. The excellent antibacterial and anticancer results prove that the bi-metal (Ag and Au) doping can enhance the biomedical properties of ZnO NPs. It will be a promising material in the biomedical field.

Ornamental morphology of ionic liquid functionalized ternary doped N, P, F and N, B, F-reduced graphene oxide and their prevention activities of bacterial biofilm-associated with orthopedic implantation.

The multifunctional biological active material design for bone tissue engineering is essential to induce osteoblast cell proliferation and attachment. Adhesion of bacteria on biomaterials to produce biofilms can be major contributors to the pathogenesis of implant material associated infections. This research work focuses on NPF& NBF elemental doping and functionalization of reduced graphene oxide using an imidazolium-based ionic liquid such as BMIM PF6 and BMIM BF4 by hydrothermal method. The resulting tri doped reduced graphene oxide (NPF-rGO and NBF-rGO) composite was further used as a scaffold for bone tissue engineering and anti-biofilm activities. The observation of the effect of NPF-rGO and NBF-rGO on the morphology, adhesion and cell proliferation of HOS cell was investigated. Moreover, the tri doped composite tested its antibiofilm properties against B. subtilis, E. coli, K. pneumoniae, and P. aeruginosa pathogenic bacteria. In-vitro studies clearly show the effectiveness of N, P, B, and F doping promoting the rGO mineralization, biocompatibility, and destruction of bacterial biofilm formation. The result of this study suggests that NPF-rGO and NBF-rGO hybrid material will be a promising scaffold for bone reaeration and implantation with a minimal bacterial infection.