ABSTRACT
Several industries have become major contributors to waterbody contamination due to the improper removal of dyes and effluents into water bodies. Due to their carcinogenic properties and low biodegradability, dye degradation is a considerable danger to people, animals, and the oceanic environment. As part of this study, Andrographis paniculata leaf extract was used as a reducing and stabilizing agent to synthesize zinc nanoparticles and degrade dyes such as methyl red and eosin. Zinc oxide nanoparticles (ZnONPs) showed a surface plasmon resonance peak at 430 nm in the UV spectrum. The FTIR result showed a band at 597.93 cm-1 that confirmed the formation of zinc nanoparticles. AFM results revealed spherical ZnONPs. The SEM results predicted an average particle size of 60 nm for crystalline particles. Biologically synthesized zinc nanoparticles exhibited greater antibacterial activity against Pseudomonas spp. and Proteus spp. but lesser activity against Klebsiella spp. and S. aureus. At 1000 µg/ml concentration, ZnONPs had the highest antioxidant activity of 45.34%. An ultraviolet-visible spectrophotometer measured dye degradation progress between 300 and 800 nm. For methyl red, the maximum absorption peak was measured at 415 nm, and for eosin, the maximum peak value was measured between 500 and 515 nm.
ABSTRACT
The present study deals with extracellular synthesis and characterization of copper sulfide (CuS) nanoparticles using Aeromonas hydrophila, and the biological applications of the synthesized CuS like antibacterial, anti-inflammatory, and antioxidant activity were reported. Further, the toxicological effects of the CuS were evaluated using zebrafish as an animal model. The primary step of the synthesis was carried out by adding the precursor copper sulfates to the culture supernatant of Aeromonas hydrophila. The UV-visible spectrophotometer was used to characterize the synthesized nanoparticles, and the peak was obtained at 307 nm through the reduction process. Fourier transform infrared spectroscopy (FTIR) was involved to find out the functional groups (carboxylic acid, alcohols, alkanes, and nitro compounds) associated with copper sulfide nanoparticles (CuS-NPs). Atomic force microscopy (AFM) was used to characterize the CuS topographically, and a scanning electron microscope (SEM) revealed about 200 nm sized CuS nanoparticles with agglomerated structures. Overall, the characterized nanoparticles can be considered as a potential candidate with therapeutic proficiencies as antibacterial, antioxidant, and anti-inflammatory mediator/agents.
Subject(s)
Aeromonas hydrophila/metabolism , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/toxicity , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/toxicity , Antioxidants/chemistry , Antioxidants/toxicity , Copper/chemistry , Copper/toxicity , Metal Nanoparticles/chemistry , Metal Nanoparticles/toxicity , Sulfides/chemistry , Sulfides/toxicity , Zebrafish/metabolism , Animals , Cell Culture Techniques/methods , Copper Sulfate/metabolism , Erythrocytes/drug effects , Humans , Inhibitory Concentration 50 , Microbial Sensitivity Tests , Microscopy, Atomic Force/methods , Microscopy, Electron, Scanning/methods , Models, Animal , Particle Size , Spectroscopy, Fourier Transform Infrared/methods , Zebrafish/embryologyABSTRACT
BACKGROUND: Because of its diverse range of use in several ethics of diagnosis and care of multiple diseases, nanotechnology has seen remarkable growth and has become a key component of medical sciences. In recent years, there has been rapid advancement in medicine and biomaterials. Nanomedicine aids in illness prevention, diagnosis, monitoring, and treatment. AIM: The purpose of this work is to evaluate the antibacterial, anti-inflammatory, and cytotoxic capabilities of green produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) against wound pathogenic as reducing agents. MATERIALS AND METHODS: The plant extract of Pongamia pinnata, silver nanoparticles, and its based curcumin nanoformulations was studied in this study utilizing UV visible spectrophotometer, selected area electron diffraction (SAED), and TEM. Anti-inflammatory, antimicrobial, and cytotoxic tests were performed on silver nanoparticles with the addition of curcumin-assisted chitosan nanocomposite (SCCN). Furthermore, these produced nanocomposites were coated on clinical silk and tested for antibacterial activity. RESULTS: The produced silver nanoparticle with the addition of curcumin-assisted chitosan nanocomposite (SCCN) has significant antibacterial activities against Pseudomonas aeruginosa and staphylococcus aureus. They are as well as possess anti-inflammatory activity and furthermore prove to be biocompatible. CONCLUSION: This advancement in the field of biomaterials, which means nanocomposite, not only helps to reduce the harmful effects of pathogenic organisms while representing an environmentally benign material but it also shows to be a material with zero danger to humans and the environment.
