Subject(s)
Glucocorticoids/pharmacology , Lymphocytes/drug effects , Lymphoid Tissue/cytology , Animals , Dogs , Drug Resistance , Guinea Pigs , Humans , In Vitro Techniques , Male , Mice , RatsABSTRACT
Lymphocytes from the cervical lymph nodes of guinea pigs were incubated in medium 199 in the presence of cortizol in a concentration of 20 and 100 microng% for 24 hours. A determination was made of the lymphocyte survival and cortizol metabolism by them, and also (cytophotometrically) the nucleic acid content. There proved to be a marked reduction of cortizol metabolism by the lymphocytes from the 6th day after the administration of the encephalytogenic mixture and a marked increase of the cortizol-resistant population of lymphocytes in the guinea pigs with encephalomyelitis, and on the 17th--30th day after the administration of a complete stimulant. In a concentration of 100 microng% cortizol reduced the nucleic acid content in the lymphocytes of the intact animals, but had no influence upon the lymphocytes of both experimental groups of guinea pigs at the period of a marked increase of the cortizol-resistant population. Progesteron decreased the lympholytic action of cortizol and the metabolism of this hormone by the lymphocytes of intact guinea pigs. The capacity of progesteron to decrease the lympholytic action of cortizol was weakened in guinea pigs with encephalomyelitis and in the animals given the stimulant.
Subject(s)
Encephalomyelitis, Autoimmune, Experimental/immunology , Hydrocortisone/pharmacology , Lymph Nodes/immunology , Lymphocytes/immunology , Animals , Guinea Pigs , Hydrocortisone/metabolism , In Vitro Techniques , Lymphocytes/drug effects , Lymphocytes/metabolism , Male , Progesterone/pharmacology , RNA/metabolismABSTRACT
Lymphocytes from the cervical lymph nodes of guinea pigs were incubated in medium 199 in the presence of cortizol in a concentration of 20 and 100 microng% for 24 hours. A determination was made of the lymphocyte survival and cortizol metabolism by them, and also (cytophotometrically) the nucleic acid content. In the intact and the sesitized guinea pigs progesterone in a concentration of 10-5 inhibited both the lytic action of coritizol and its metabolism There was observed a marked reduction of cortizol meta bolism by the lymphocytes from the 6th day after the sensitization, which was restored only by the 90th day. The cortizol-resistant lymphocyte population increased on the 17th--30th day. In a concentration of 100 microng% cortizol reduced the nucleic acid content in the lymphocytes of both the intact guinea pigs and sensitized ones on the 17th--30th day, when the cortizol-resistant population increased.
