ABSTRACT
It was shown possible to use feeding of hydrogen peroxide as a method for investigating the impact of dissolved oxygen concentrations on growth and development of microorganisms. The influence of pO2 on the respiration intensity was studied in penicillin- and erythromycin-producing cultures and it was found that dependence of the respiration intensity on pO2 had the form of a curve with saturation, at pO2 equal to zero the value of the culture respiration intensity being different from zero. A mathematical model accounting for the presence in the fermentation broth of microbial agglomerates with the average size depending on the agitation conditions is proposed for describing the relationships.
Subject(s)
Erythromycin/biosynthesis , Oxygen Consumption , Oxygen/administration & dosage , Penicillins/biosynthesis , Penicillium/drug effects , Streptomyces/drug effects , Air , Culture Media , Fermentation/drug effects , Fermentation/physiology , In Vitro Techniques , Penicillium/growth & development , Penicillium/metabolism , Solutions , Streptomyces/growth & development , Streptomyces/metabolismABSTRACT
The use of vibroagitation in biosynthesis of antibiotics was shown to be in principle possible. The results of the experiments in 3-liter apparatus equipped with devices for vibroagitation and turbine mixers are presented.