ABSTRACT
[68Ga]Ga-FAPI-46 is a promising new tracer for the imaging of fibroblast activation protein (FAP) by positron emission tomography (PET). Labeled FAP inhibitors (FAPIs) have demonstrated uptake in various types of cancers, including breast, lung, prostate, pancreatic and colorectal cancer. FAPI-PET also possesses a practical advantage over FDG-PET as fasting and resting are not required. [68Ga]Ga-FAPI-46 exhibits enhanced pharmacokinetic properties, improved tumor retention, and higher contrast images than the earlier presented [68Ga]Ga-FAPI-02 and [68Ga]Ga-FAPI-04. Although a manual synthesis protocol for [68Ga]Ga-FAPI-46 was initially described, in recent years, automated methods using different commercial synthesizers have been reported. In this work, we describe the development of the automated synthesis of [68Ga]Ga-FAPI-46 using the iPHASE MultiSyn synthesizer for clinical applications. Initially, optimization of the reaction time and comparison of the performance of four different solid phase extraction (SPE) cartridges for final product purification were investigated. Then, the development and validation of the production of 0.6-1.7 GBq of [68Ga]Ga-FAPI-46 were conducted using these optimized parameters. The product was synthesized in 89.8 ± 4.8% decay corrected yield (n = 6) over 25 min. The final product met all recommended quality control specifications and was stable up to 3 h post synthesis.
Subject(s)
Gallium Radioisotopes , Radiopharmaceuticals , Gallium Radioisotopes/chemistry , Radiopharmaceuticals/chemical synthesis , Radiopharmaceuticals/chemistry , Humans , Positron-Emission Tomography/methods , Membrane Proteins/antagonists & inhibitors , EndopeptidasesABSTRACT
We measured the excitation functions of (nat)Zn (p,x) reactions up to 17.6MeV, using the stacked-foils activation technique. High-purity natural zinc (and copper) foils were irradiated with proton beams generated by an 18MeV isochronous cyclotron. Activated foils were measured using high-purity Ge gamma spectroscopy to quantify the radionuclides (61)Cu, (66)Ga, (67)Ga, and (65)Zn produced from the reactions. Thick-target integral yields were also deduced from the measured excitation functions of the produced radioisotopes. These results were compared with the published literature and were found to be in good agreement with most reports, particularly those most recently compiled.
Subject(s)
Cyclotrons , Energy Transfer , Protons , Spectrometry, Gamma/methods , Zinc/chemistry , Zinc/radiation effects , Materials Testing , Radiation DosageABSTRACT
This study examined the response characteristics of three commercially available radiochromic films when exposed to low energy (50 kVp) X-rays. The aim was to evaluate the films for potential use in 2D dosimetry for a low-kV intraoperative radiotherapy (IORT) device known as the 'Intrabeam'. Dose-response relationships were obtained for Gafchromic EBT, XR-RV2, and XR-QA film in water at several distances from the Intrabeam device. It was found that the dose rates from the source were excessive for use of the XR-QA film, and that all three film types showed significant energy dependence within the limits of measurement uncertainty. Basic modeling of primary X-ray spectra indicated large changes in the lower energy components with distance from the source, and it is hypothesized that the changes in film response are a result of changes in film energy response. This is in contrast to previous studies indicating less or negligible energy response. All films showed non-linearity in response over the ranges examined. These results imply significant limitations for the use of these films for low-kV dosimetry.
