ABSTRACT
BACKGROUND: Amyloid-ß peptide (Aß) is involved in the formation of senile plaques in Alzheimer's disease (AD), and causes neuronal cell death by inducing oxidative stress. OBJECTIVE: We investigated the protective effect of Eremostachys labiosiformis extract against the Aß-induced toxicity in SH-SY5Y cells. METHODS: Methanolic extract from the aerial parts of E. labiosiformis was prepared by percolation method at room temperature. SH-SY5Y cells were treated and incubated with different concentrations of the extract for 1 h, before addition of Aß. Cytotoxicity was measured 24 h after the addition of Aß to the medium using MTT and reactive oxygen species (ROS) assays. Effective doses were evaluated using real-time polymerase chain reaction to evaluate expression of miR-212 and miR-132. The results were analyzed using SPSS software (16). RESULTS: Exposure of -SH-SY5Y cells to Aß significantly affected the viability of cells and increased ROS levels. The results revealed that 1.2 and 2.5 µg/mL of the E. labiosiformis extract reduced Aß-induced deterioration. Only 2.5 µg/mL of the extract could reduce ROS levels. In addition, 5 µg/mL of the extract increased the expression of the miRNAs, which was reduced after exposure to Aß. CONCLUSION: Based on the antioxidant and protective effects of the E. labiosiformis extract on expression of miR-132 and miR-212 and ROS level, this herb could be used as a suitable candidate for future studies on neurodegenerative diseases including AD.
