ABSTRACT
Twenty six Newcastle disease viruses--12 reference strains and 14 strains isolated in Kenya and Kazakhstan--were characterized by means of a large panel of 38 monoclonal antibodies (MAB) directed against all the three envelope proteins: matrix, hemagglutinin-neuraminidase and fusion. The essential distinctions were revealed between the viruses isolated in Kenya and Kazakhstan while the differences amongst the viruses belonging to the same local group were much smaller. The heterogeneity amongst the viruses isolated in Kenya was more expressed as compared to the Kazakhstanian strains.
Subject(s)
Bird Diseases/virology , Newcastle disease virus/classification , Newcastle disease virus/immunology , Poultry Diseases/virology , Animals , Antibodies, Monoclonal , Antibodies, Viral/immunology , Antigenic Variation , Antigens, Viral/immunology , Birds , Disease Outbreaks/veterinary , Kazakhstan , Kenya , PoultryABSTRACT
Nine monoclonal antibodies (MAB) against nucleocapsid protein (NP) of Newcastle disease virus (NDV) have been prepared and characterized. All the MABs were classified into three groups by means of the competitive binding assay. At least three antigenic sites were delineated on the NP. The 1st site includes two closely located epitopes; the 2nd site includes two related and two distinct epitopes; the 3rd site includes two closely related and one distinct epitopes.
Subject(s)
Antibodies, Viral/immunology , Antigens, Viral/immunology , Newcastle disease virus/immunology , Nucleoproteins/immunology , Viral Proteins/immunology , Antibodies, Monoclonal , Antigens, Viral/classification , Binding, Competitive , Immunoglobulin Isotypes , Newcastle disease virus/classification , Nucleocapsid Proteins , Nucleoproteins/classification , Species Specificity , Viral Proteins/classificationABSTRACT
From domestic birds 13 strains of avian paramyxoviruses, serotype 1, and 14 strains of serotype 2 were isolated. Avian paramyxoviruses, serotype 2, differ antigenically and biologically from each other and from the prototype variant chicken/Yucaipa/California/56. The virus was also detected experimentally in birds having contact with the infected specimens. Examinations of avian blood sera revealed wide dissemination of viruses related to the chicken/Yucaipa/56 strain in domestic bird breeding farms (43.6% to 50.0% of positive birds). The detected variability of the antigenic structure of the isolates attests to the potential emergence of a pathogenic variant.
