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1.
Determination of cancer allelotype.
Ascaño, Jennifer J; Powell, Steven M.
Methods Mol Biol ; 222: 295-307, 2003.
Article in English | MEDLINE | ID: mdl-12710694

Subject(s)
Genes, Tumor Suppressor , Neoplasms/genetics , Electrophoresis, Polyacrylamide Gel/methods , Fluorescent Dyes , Genotype , Microsatellite Repeats , Polymerase Chain Reaction/methods , Staining and Labeling/methods
2.
Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment.
Shuber, Anthony P; Ascaño, Jennifer J; Boynton, Kevin A; Mitchell, Anastasia; Frierson, Henry F; El-Rifai, Wa'el; Powell, Steven M.
J Clin Microbiol ; 40(1): 262-4, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11773127

ABSTRACT

A novel DNA assay demonstrating sensitive and accurate detection of Helicobacter pylori from stool samples is reported. Moreover, in three individuals tested for therapeutic response, the assay showed the disappearance of H. pylori DNA during treatment. Thus, this noninvasive molecular biology-based assay has the potential to be a powerful diagnostic tool given its ability to specifically identify H. pylori DNA.


Subject(s)
DNA, Bacterial/analysis , Feces/microbiology , Helicobacter Infections/diagnosis , Helicobacter pylori/isolation & purification , Polymerase Chain Reaction/methods , Helicobacter Infections/microbiology , Helicobacter pylori/genetics , Humans , Sensitivity and Specificity
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