1.
Fertil Steril
; 83(1): 246-9, 2005 Jan.
Article
in English
| MEDLINE
| ID: mdl-15652923
ABSTRACT
The first two human embryonic stem cell lines (VAL-1 and VAL-2) have been derived in Spain with long-term cryopreserved embryos under animal-free conditions. In the first series, 40 human embryos that had been cryopreserved at day 2 of development were thawed after >5 years. A derivation efficiency of 5% per frozen embryo or 12.5% per blastocyst was obtained.