Comparative toxicity of nanoparticulate and ionic copper following dietary exposure to common carp (Cyprinus carpio).

Copper is an essential element for the normal growth and survival of all organisms including fish. However, its excessive presence in the environment can cause bioaccumulation and aquatic toxicology. The aim of the present study was to compare the dietary toxicity effects of two different Cu compounds, copper oxide nanoparticles (CuO-NPs) and ionic copper (CuSO4) in juvenile common carp, Cyprinus carpio. To prepare experimental diets, two nominal concentrations of 100 and 1000 mg Cu kg-1 diet were added to a basal diet. Carp (n = 450, average initial weight of 35.94 ± 5.35 g) were fed on the Cu-supplemented diets and basal diets for two 21-day courses as dietary exposure and recovery periods, respectively. The growth performance, survival rate and blood biochemical indices as well as copper accumulation in target organs of fish were investigated at the end of each exposure period. The results showed that the weight gain (WG) of carp significantly decreased coincident with increasing concentration of the both dietary Cu forms (P = 0.00). Both Cu sources at concentrations of 100 mg kg-1 diet decreased the survival rate of fish (P = 0.003), likely due to more feed intake and thus increased copper toxicity. The both forms of dietary Cu at two different concentrations significantly decreased the plasma glutamate oxaloacetate transaminase (GOT) level compared to the control group (P = 0.008). Fish exposed to diets containing Cu sources except 100 mg Cu kg-1 of CuO-NPs showed the lower glutamate pyruvate transaminase (GPT) activity in comparison to the control (P = 0.00). The plasma sodium level in1000 mg CuO-NPs kg-1 diet was significantly lower than the control (P = 0.001). The plasma potassium level increased in the all Cu-supplemented groups except 100 mg kg-1 of CuO-NPs after the dietary exposure period (P = 0.035). The copper accumulation was dose-dependent in all target organs. In 100 mg Cu kg-1 dietary groups, the liver showed the highest Cu accumulation (P = 0.00), while in 1000 mg Cu kg-1 dietary groups, the highest Cu content was observed in the intestine (P = 0.00). The results demonstrated the enhanced toxicological responses in fish after 21 days of dietary exposure, but the levels of most of biochemical indices and tissues Cu content decreased or returned to the control values after the recovery period.

Introducing a new standardized nanomaterial environmental toxicity screening testing procedure, ISO/TS 20787: aquatic toxicity assessment of manufactured nanomaterials in saltwater Lakes using Artemia sp. nauplii.

This paper introduces a new standardized testing procedure for nanomaterial environmental toxicity (International Organization for Standardization/Technical Specification (ISO/TS) 20787): 'aquatic toxicity assessment of manufactured nanomaterials in saltwater lakes using Artemia sp. Nauplii' intended to generate more reliable and repeatable aquatic toxicity data testing manufactured nanomaterials, using Artemia sp., to evaluate their possible ecotoxicity in saltwater lake ecosystems. The principles behind testing with Artemia sp. are reviewed and the paper gives an overview of research published between 2009 and 2018 in which manufactured nanomaterials were tested using Artemia sp.

Acute toxicity, uptake, and elimination of zinc oxide nanoparticles (ZnO NPs) using saltwater microcrustacean, Artemia franciscana.

This study aims to evaluate the potential toxic effects of ZnO nanoparticles on Artemia franciscana nauplii. The ZnO NPs suspension was characterized by TEM, EDS and DLS techniques. Acute toxicity was investigated by exposure of nauplii to concentrations of 1, 5, 7.5, 10, 15, 20, 25 and 30 mg/L of ZnO NPs for 48 h and 96 h. The 96-h EC10 and EC50 values of ZnO NPs were found to be 1.39 mg/L and 4.86 mg/L respectively. The ZnO NPs suspensions did not cause any significant acute toxicity after 48 h of exposure, but the immobilization rate increase significantly compare to control group after 96 h (P < 0.05). The results showed that the uptake, accumulation, and elimination of NPs in nauplii depends on the concentration of NPs and time. The elimination rates of 46.66% and 83.85% were recorded at 1 and 10 mg/L of NPs after 24 h of depuration period, respectively.

Comparison of one step macular hole and cataract surgery with two step surgery / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To compare one step macular hole and cataract surgery with two step surgery in patients with macular hole and cataract. ●METHODS: ln a clinical trial conducted on patients suffering from cataract with macular holes inTabriz, the effects of simultaneous surgery of macular hole and cataract performed on these patients were studied. ln this study, 22 patients ( Group A ) were, first, undergone phacoemulsification and intraocular lens embedment, and one month later, 23-gauge pars plana vitrectomy together with the retinal internal limiting membrane ( lLM ) removal. Twenty - one patients ( Group B ) were undergone simultaneous phacoemulsification, intraocular lens embedment, 23-gauge pars plana vitrectomy, and the retinal lLM removal. ●RESULTS: Group A was comprised of 7 males and 15 females, and Group B of 9 males and 12 females ( P=0. 545) . The mean age of patients in Group A and B were 66.63±4. 75 and 67. 71±4. 99y, respectively (P=0. 472). Three months after surgeries were performed, the macular holes of 2 patients from Group A and 5 patients from Group B were open ( P = 0. 240 ). Uveitis was observed in 1 patient from Group A and 4 from Group B (P=0. 185). The increase of intraocular pressure (lOP) was merely observed in 2 patients from Group B ( P=0. 233 ) . Posterior capsule opacification ( PCO ) was observed, three months after surgeries, in 2 patients from Group A and 4 from Group B (P=0. 412). ● CONCLUSION: The results suggested that, no significant difference existed between patients from the two groups in terms of outcome and postoperative complications, although,Group B patients, the recipients of simultaneous surgeries, experienced more complications compared to their Group A counterparts.

Toxicity of various silver nanoparticles compared to silver ions in Daphnia magna.

BACKGROUND: To better understand the potential ecotoxicological impacts of silver nanoparticles released into freshwater environments, the Daphnia magna 48-hour immobilization test was used. METHODS: The toxicities of silver nitrate, two types of colloidal silver nanoparticles, and a suspension of silver nanoparticles were assessed and compared using standard OECD guidelines. Also, the swimming behavior and visible uptake of the nanoparticles by Daphnia were investigated and compared. The particle suspension and colloids used in the toxicity tests were well-characterized. RESULTS: The results obtained from the exposure studies showed that the toxicity of all the silver species tested was dose and composition dependent. Plus, the silver nanoparticle powders subsequently suspended in the exposure water were much less toxic than the previously prepared silver nanoparticle colloids, whereas the colloidal silver nanoparticles and AgNO(3) were almost similar in terms of mortality. The silver nanoparticles were ingested by the Daphnia and accumulated under the carapace, on the external body surface, and connected to the appendages. All the silver species in this study caused abnormal swimming by the D. magna. CONCLUSION: According to the present results, silver nanoparticles should be classified according to GHS (Globally Harmonized System of classification and labeling of chemicals) as "category acute 1" to Daphnia neonates, suggesting that the release of nanosilver into the environment should be carefully considered.