Effects of black pepper and turmeric powder on growth performance, gut health, meat quality, and fatty acid profile of Japanese quail.

In poultry production, the search for alternatives to in-feed antibiotics continues unabated. This study investigated the effects of dietary supplementation of black pepper and turmeric powder, separately or in combination, on the growth performance, gastrointestinal microbiota population, intestinal morphology, serum biochemical parameters, meat quality, and meat fatty acid profile in Japanese quails. Five hundred-day-old mixed-sex Japanese quail chicks were randomly assigned to one of five treatments: a control diet (CON); CON +0.2% antibiotic flavomycin as an antibiotic growth promoter (AGP); CON +0.5% turmeric powder (TUP); CON +0.5% black pepper powder (BPP); and CON +0.5% TUP, and 0.5% BPP (MIX). The findings showed that quail chicks fed AGP and TUP throughout the rearing period had better body weight gain (p = 0.007) and feed conversion ratio (p = 0.02) than the other treatments. The TUP, BPP, and MIX feeds reduced (p = 0.005) abdominal fat percentage. The MIX group had a better breast muscle water-holding capacity (p = 0.04) and lightness index (p = 0.02) and lower (p = 0.02) malondialdehyde concentration after 7 days of refrigerated storage. Feeding BPP, TUP, and MIX diets decreased (p = 0.001) serum cholesterol concentration. Quail chicks fed the CON diet showed significantly higher coliform counts in the crop and ileum (p < 0.001), whereas the lactic acid bacterial population was lower (p = 0.008) in the ileum. Birds that received the MIX diet exhibited a higher (p = 0.02) villus height to crypt depth ratio in the duodenum compared to the other groups. The tested feed additives increased (p < 0.001) villus height in the jejunum and ileum compared to other groups. Feeding the TUP, BPP, and MIX diets reduced (p < 0.001) total saturated fatty acid content and increased (p = 0.004) total polyunsaturated fatty acid concentration, where the MIX diet had the best results. Overall, the present data indicate that supplementing the basal diet with turmeric powder enhances the growth performance of Japanese quails. In some respects, such as gut health and meat quality, combining turmeric powder and black pepper powder was more effective than using them independently.

In ovo injection of silver nanoparticles modulates some productive traits and hepatic gene expression of broilers exposed to lipopolysaccharide challenge.

This study aimed to evaluate the effectiveness of the embryonic injection of silver nanoparticles (SilNPs) on some productive traits and hepatic gene expression of lipopolysaccharide (LPS)-challenged broilers after a 42 d rearing period. 560 fertile eggs were randomly allocated to four groups and received either of the following treatments at d 7 of incubation, control (no injection), placebo (1 mL saline), SilNP20 (20 mg/kg silver nanoparticles), or SilNP40 (40 mg/kg silver nanoparticles). After the incubation, 320 hatchlings experienced a 42 d standard rearing period. Live body weight (LBW), feed intake (FI), and feed conversion ratio (FCR) were weekly recorded. At the end of the experiment, two birds from each replicate (n = 8 per treatment) were exposed to LPS intraperitoneal injection at 48, 24, and 12 h before slaughter time. They were also used for blood, intestinal, and microbial evaluations. The hepatic mRNA levels of tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), transforming growth factor beta (TGF-ß), and insulin-like growth factor I (IGF-I) were assessed at d 1 and 42 of the experiment. Adminstration of SiLNPs improved LBW, FI, and FCR and also enhanced liver and spleen weights (P < 0.05). SilNP20 birds had significantly lower bursa of Fabricius weight (P < 0.05). SilNP20 had lower total cholesterol levels than others. There was a significant difference (P < 0.05) between SliNP40 and SilNP20 in the ratio of villus height to crypt width. Compared to control groups, chicks of SilNP20, but not SilNP40, showed a significant increase in the relative expression of TNF-α, IL-6, TGF-ß, and IFG-I genes at d 1. On d 42, however, both SilNP20 and SilNP40 had significantly higher TNF-α and TGF-ß levels than both controls. Silver nanoparticles did not significantly affect the microflora of the ileum and cecum in the current study. In summary, SilNPs administration to chick embryos showed a long-term positive effect on their productive performance.

The effect of fermented rapeseed meal on production performance, egg quality and hatchability in broiler breeders after peak production.

1. This experiment was conducted to evaluate the effects of replacing soybean meal (SBM) with different levels of rapeseed meal (RSM) or fermented rapeseed meal (FRSM) on performance, egg quality and hatchability in broiler breeders.2. RSM was fermented with Bacillus subtilis and Aspergillus niger for 25 d. A total of 140 Ross 308 broiler breeder hens (52 weeks old; 20 per treatment, four hens per cage replicate) were assigned to seven dietary treatments in a completely randomised design involving a control (maize-SBM diet) and a 2 × 3 factorial arrangement, consisting of two types of meal (RSM and FRSM) and three replacement levels (33%, 66% or 100%) for SBM, with one cockerel housed within each cage replicate for 12 weeks.3. Fermentation increased the population of lactic acid bacteria and crude protein and decreased pH, dry matter, crude fibre and anti-nutrients in FRSM meal compared to RSM (P < 0.05).4. Body weight gain and egg weight were not affected by RSM or FRSM. Feeding FRSM compared to RSM increased egg production and egg mass (P < 0.05). FRSM or RSM at the level of 100% significantly decreased egg production and egg mass compared to the control group (P < 0.05). Neither types of meal nor levels of replacing significantly affected egg quality variables except eggshell strength. Yolk colour in all experimental treatments was significantly higher than the control group (P < 0.05). By increasing the level of RSM or FRSM in the diet, hatching rate and chicken weight decreased (P < 0.05).5. Microbial fermentation improved the nutritional value of RSM which could be used to replace SBM with RSM or FRSM at levels lesser than 66% as a suitable protein alternative for broiler breeders.

Study on the chemical and microbial composition and probiotic characteristics of dominant lactic acid bacteria in fermented poultry slaughterhouse waste.

This study aimed to evaluate the quality of fermented poultry slaughterhouse waste and isolate and identify the probiotic bacteria present in it. Poultry slaughterhouse waste samples supplemented with 5% starter culture and 0.2% propionic acid received one of four experimental treatments, which consisted of further supplementation with: (1) 10% molasses (M10); (2) 15% molasses (M15); (3) 10% molasses with 200ppm butylated hydroxytoluene (MB10); and (4) 15% molasses with 200ppm butylated hydroxytoluene (MB15). All experimental treatments (silages) were fermented for 6days with sampling before fermentation (day 1) and at the end of days 3 and 6. The pH of the silages decreased during the fermentation as it was lower on days 3 and 6 than day 1 (P<0.05). On day 6 of the experiment, the number of lactic acid bacteria in MB15 was significantly (P<0.05) higher than in MB10. The percentage of non-protein nitrogen increased (P<0.05) on days 3 and 6 of fermentation. The amount of malondialdehyde indicated that the peroxidation ratio decreased (P<0.05) with increasing the fermentation time. Among the 23 lactic acid bacteria detected in MB15, nine isolates (39.1%) belonged to the species Lactobacillus plantarum, ten (43.5%) belonged to the species Lactobacillus rhamnosus, and four (43.5%) belonged to the species Lactobacillus fermentum. The isolates LPL9, LRH25, and LFE26 were identified as the strain of L. plantarum, L. ramnosus, and L. fermentum, respectively, that demonstrated the best characteristics for use as a probiotic. The results of this experiment indicated that the fermentation is an advisable method for reusing the nutrients of poultry slaughterhouse waste and producing a probiotic supplement.

Effect of dietary antibiotic, probiotic and prebiotic as growth promoters, on growth performance, carcass characteristics and hematological indices of broiler chickens.

This experiment was conducted for comparison the effects of antibiotic (flavomycin), probiotic (primalac), prebiotic (Biolex-MB) and mixture ofprobiotic and prebiotic (primalac plus Biolex-MB) as dietary growth promoter on growth performance, carcass characteristics and hematological indices of broiler chickens. Three hundred day old Ross 308 broilers were equally distributed into 30 floor pens and reared for 42 day. A basal diet was formulated covering the recommendations of NRC (1994) for starter (0-21 days) and grower (22-42 days) periods and considered as control diet. Four tested diets were formulated by supplemented the basal control diet with antibiotic (flavomycin), probiotic (primalac), prebiotic (Biolex-MB) and mixture ofprimalac plus Biolex-MB, respectively. Six replicates were used for each treatment. The results of present study showed that all growth promoters used was improved growth indices of Ross 308 broilers. The highest significant (p<0.05) values of carcass and thigh were recorded for broilers fed diet supplemented with flavomycin. The highest (p>0.05) value of breast was recorded for broilers fed the diet supplemented with primalac, meanwhile the lower value were showed for birds fed either diet or diet supplemented with Biolex-MB. The percent of carcass and cuts followed the same trend. Hematological parameter including cholesterol was recorded the highest (p>0.05) values groups fed the diets either control or supplemented with flavomycin, meanwhile the lower value was showed for bird fed diet supplemented primalac plus Biolex-MB. Triglycerides and very low density lipoprotein cholesterol (VLDL) were recorded the highest concentration for bird fed both control and diet supplemented with flavomycin groups while least concentration was found for bird fed diet supplemented with primalac. The results of present study revealed that probiotic and prebiotic as growth promoters can use as alternatives non-antibiotic feed additives to their free harmful side effects on the consumers and to improve broiler chickens growth indices.