ABSTRACT
Carboxyl-functionalized molybdenum disulfide (COOH-MoS2) nanosheets were prepared through a facile low-temperature hydrothermal method. The phase transformation of metallic-1T to 2H-semiconductor COOH-MoS2 nanosheets was conducted through introducing Au thin film on the unclad optical fiber as a sensing layer in a low temperature. The developed structure successfully refined the loss of the semiconducting properties and poor adhesion of COOH-MoS2 on the unclad polymer optical fiber, which provided limited semiconductor potential as the sensing layers on the optical fiber surfaces. The sensing performance of the as-prepared structure was tested for quantitative detection of three different volatile organic carbons (VOCs) of ethanol, propanol, and methanol gases as well as cross-sensitivity to relative humidity. The operating principle was based on intensity variation of the evanescent wave in the sensing region. The response of the proposed sensing system shows maximum response and better linearity (R2 = 0.999) to methanol at room temperature. Finally, the comparative experimental cross-sensitivity to relative humidity and methanol was also studied to evaluate the potential of sensing range.
ABSTRACT
Silymarin (SMN) is used as an antioxidant complex to attenuate the pro-oxidant effects of toxic agents. This study was carried out to investigate the effect of SMN, Celecoxib (CLX) individually and in combination on monoiodoacetate (MIA)-induced osteoarthritis (OA) in rat. Forty adult Wistar rats were assigned to control and test groups. Animals in the test group following OA induction were subdivided into 4 subgroups according to the treatment profile: OA(+); received saline normal (5ml/kg, b.w.), OA(+)CLX(+); received CLX (100mg/kg, orally), OA(+)SMN(+), received SMN (50mg/kg, orally), and OA(+)CLX(+)SMN(+), received both CLX and SMN. The animals received test compounds by gastric gavage for 14 consecutive days. Animals in the OA(+) group showed a significant (p<0.01) increase in serum and synovial levels of IL-1ß, while both test compounds reduced the IL-1ß level. Both CLX and SMN lowered the OA-increased level of malondialdehyde by 77% and 79% and nitric oxide by 73% and 76%, respectively, in the synovial tissue. Special safranin O (SO) histopathological staining revealed that CLX and SMN improved the MIA-induced destruction and fibrillation in cartilage surface. CLX and SMN regulated the MIA-up regulated IL-1ß at mRNA level. The combination therapy resulted in an additive effect between CLX and SMN in biochemical, histopathological and molecular assays. These findings suggest that SMN exerts anti-inflammatory effect and also potentiates the anti-inflammatory effect of CLX on MIA-induced OA. The anti-inflammatory property of SMN may attribute to its antioxidant capacity, which affects the proinflammatory mediators at translational and transcriptional level.
