ABSTRACT
BACKGROUND: Nowadays, the biological properties and anticancer activities of platinum-based drugs and metal coordination complexes have been receiving particular attention. These compounds have revealed clinical potential in cancer chemotherapy. OBJECTIVE: In this research, two binuclear platinum complexes including [Pt2Cl2(bhq)2(µ-dppm)] (1) and [(p- MeC6H4)(bhq) Pt(µ-dppm)Pt(bhq)(CF3CO2)] (2) with bhq: benzo[h] quinolone and dppm: bis(diphenylphosphino) methane have been synthesized and evaluated for their anticancer activity against A2780 and A2780/RCIS cancer cell lines. METHODS: The DNA binding and interaction of AMP/GMP nucleotide with these complexes were explored by several experimental and theoretical methods, including UV-Visible, fluorescence spectroscopic techniques and docking analysis. These complexes have demonstrated significant anticancer properties against cisplatinsensitive (A2780) and cisplatin-resistant (A2780/RCIS) human ovarian cancer cell lines. RESULTS: The obtained results indicated that these complexes interact with DNA. Additionally, the fluorescence emission measurements indicated that the platinum complexes binding with DNA structure occurs through nonintercalative interaction. The molecular docking assessments have also revealed the binding of these platinum complexes through DNA grooves. Moreover, the results have indicated that complex 1 exhibited more anticancer activity than complex 2. CONCLUSION: The results of the DNA binding with these platinum complexes confirmed their potential antitumor properties. The substitution of -C6H4CH3 and -CO2CF3 groups in complex 2 with two chlorine atoms in complex 1 acquired the significant improvement of the anticancer activity against the cancer cell.
Subject(s)
Antineoplastic Agents/pharmacology , DNA/drug effects , Molecular Docking Simulation , Organoplatinum Compounds/pharmacology , Spermatozoa/drug effects , Animals , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Binding Sites/drug effects , Circular Dichroism , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Fishes , Male , Molecular Structure , Organoplatinum Compounds/chemical synthesis , Organoplatinum Compounds/chemistry , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , Structure-Activity RelationshipABSTRACT
The interactions between eight amino acid based anions and four imidazolium-based cations have been investigated by density functional theory. The electronic and structural properties of the resulting amino acid ionic liquids (AAILs) have been unveiled by means of the atoms in molecules framework. The calculated interaction energy was found to increase in magnitude with decreasing alkyl chain length at imidazolium ring. Moreover, AAILs composed of an amino acid with some functional group such as aromatic ring had decreased interaction energy. Finally, several correlative relationships between glass transition temperature and interaction energy as well as density at bond critical point have been checked for 1-ethyl-3-methylimidazolium based ILs. Although the obtained correlations do not show excellent fits, a preliminary estimation of the glass transition temperature of different AAILs can be achieved by use of their electronic properties.
