ABSTRACT
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the gastrointestinal tract. The authors take this opportunity to report two cases of GISTs of large bowel diagnosed on cytology and confirmed by histopathology and immunohistochemistry.
Subject(s)
Abdomen/pathology , Gastrointestinal Stromal Tumors/diagnosis , Abdomen/diagnostic imaging , Adult , Biomarkers, Tumor/chemistry , Cell Nucleus Shape , Cytodiagnosis , Cytoplasm/chemistry , Cytoplasm/pathology , Endoscopic Ultrasound-Guided Fine Needle Aspiration , Gastrointestinal Stromal Tumors/chemistry , Humans , Immunohistochemistry , Male , Middle Aged , Proto-Oncogene Proteins c-kit/chemistryABSTRACT
The objective of this study was to probe the apoptotic effect of green tea polyphenol epigallocatechin-3-gallate (EGCG) on cervical carcinoma cells. This study was conducted in Departments of Pathology and Biochemistry, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India over a span of one and half years from January 2005 to August 2006. Caspase-3 assay was performed on monocytes isolated from cervical carcinoma patients and cultured with EGCG; cytosmears and sections from cervical carcinoma tissue cultured with EGCG were prepared for the morphological evidence of apoptosis. EGCG in a dose of 5 µg/ml and 10 µg/ml increased the caspase-3 levels in human cells. Cytosmears and sections from cervical carcinoma tissue cultured with EGCG showed better differentiation and increased number of apoptotic cells as compared to non EGCG controls. The number of such cells was increased more in 48 hours than in 24 hours. EGCG in a dose of 5 µg/ml and 10 µg/ml promoted apoptotic preparedness of human cells and induced apoptotic change in cervical carcinoma cells.
Subject(s)
Adenocarcinoma/drug therapy , Antioxidants/pharmacology , Apoptosis/drug effects , Catechin/analogs & derivatives , Uterine Cervical Neoplasms/drug therapy , Adenocarcinoma/blood , Adenocarcinoma/pathology , Caspase 3/metabolism , Catechin/pharmacology , Cell Line, Tumor , Drug Screening Assays, Antitumor , Female , Humans , Leukocytes, Mononuclear/drug effects , Leukocytes, Mononuclear/enzymology , Leukocytes, Mononuclear/pathology , Uterine Cervical Neoplasms/blood , Uterine Cervical Neoplasms/pathologyABSTRACT
In spite of becoming an integral part of surgical pathology, very few reports are available regarding the utility of intraoperative cytology (IOC) exclusively for bone lesions. This study was undertaken in a view to fill this lacuna. Sixty bone lesions were evaluated intraoperatively with the help of cytology smears prepared by touch, scrape, or crush technique. The diagnosis made on cytological preparation was compared with histopathological diagnosis taking the latter as gold standard. Different parameters like reasons for Intraoperative consultation, best technique for preparation of smear, average time taken to render a diagnosis, and finally the accuracy of IOC was evaluated. Common reasons for the intraoperative consultation were to make or confirm a diagnosis for proper surgical intervention and to evaluate the surgical resection margin. Scrape was found to be the best method for cytological smear preparation. Average time taken to render a diagnosis was 20 minutes. Sensitivity, specificity, and overall diagnostic accuracy was 96.7, 96.6, and 96.6%, respectively. Cytology can play a valuable role in the intraoperative diagnosis of bone lesions. The method is simple, cheap, quick, and has no complication. It should be undertaken routinely, as a rapid intraoperative diagnosis will expedite timely and proper management of the patients, along with early post operative treatment and thus avoid the aggravating delays.
