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1.
J Pak Med Assoc ; 73(9): 1788-1793, 2023 Sep.
Article in English | MEDLINE | ID: mdl-37817685

ABSTRACT

OBJECTIVE: To screen new-borns to diagnose any hearing impairment early. METHODS: The prospective, cross-sectionalstudy was conducted at the Department of Otorhinolaryngology, Head and Neck Surgery Liaquat National Hospital, Karachi, from November 1, 2020, to April 30, 2021, and new- borns of either gender aged >12h born via spontaneous vaginal delivery, induced labour, and Caesarean section. A predesigned questionnaire was used to collect detailed case history, including gestational age, duration of labour, and other prenatal, natal, and postnatal risk factors. Otoacoustic emission test was performed, and infants referred twice were scheduled for complete diagnostic evaluation and brainstem evoked response audiometry. Data was analysed using SPSS 23. RESULTS: Of the 267 neonates, 249(93.3%) passed the first screening. Of the remaining 18(6.7%) neonates, 8(44.4%) passed the second screening, while 10(55.5%) were asked to come for a follow-up after three weeks. Of them, 3(30%) returned for check-up, while 7(70%) did not show up. CONCLUSIONS: Neonatal risk factors associated with hearing loss need to be identified, and a comprehensive hearing screening programme is required for neonates.


Subject(s)
Developing Countries , Hearing Loss , Infant, Newborn , Infant , Humans , Female , Pregnancy , Cesarean Section , Prospective Studies , Tertiary Care Centers , Otoacoustic Emissions, Spontaneous/physiology , Neonatal Screening , Hearing Loss/diagnosis , Hearing Loss/epidemiology
2.
BMC Cancer ; 16(1): 883, 2016 11 14.
Article in English | MEDLINE | ID: mdl-27842576

ABSTRACT

BACKGROUND: The purpose of present work is to synthesize novel (+)-Dehydroabietylamine derivatives (DAAD) using N-acetyl-α-amino acid conjugates and determine its cytotoxic effects on hepatocellular carcinoma cells. METHODS: An analytical study was conducted to explore cytotoxic activity of DAAD on hepatocellular carcinoma cell lines. The cytotoxicity effect was recorded using sulforhodamine B technique. Cell cycle analysis was performed using Propidium Iodide (PI) staining. Based on cell morphology, anti growth activity and microarray findings of DAAD2 treatment, Comet assay, Annexin V/PI staining, Immunoperoxidase assay and western blots were performed accoringly. RESULTS: Hep3B cells were found to be the most sensitive with IC50 of 2.00 ± 0.4 µM against (+)-N-(N-Acetyl-L-Cysteine)-dehydroabietylamine as DAAD2. In compliance to time dependent morphological changes of low cellular confluence, detachment and rounding of DAAD2 treated cells; noticeable changes in G2/M phase were recorded may be leading to cell cycle cessation. Up-regulation (5folds) of TUBA1A gene in Hep3B cells was determined in microarray experiments. Apoptotic mode of cell death was evaluated using standardized staining procedures including comet assay and annexin V/PI staining, Immuno-peroxidase assay. Using western blotting technique, caspase dependant apoptotic mode of cell death was recorded against Hep3B cell line. CONCLUSION: It is concluded that a novel DAAD2 with IC50 values less than 8 µM can induce massive cell attenuation following caspase dependent apoptotic cell death in Hep3B cells. Moreover, the corelation study indicated that DAAD2 may have vital influence on cell prolifration properties.


Subject(s)
Abietanes/chemical synthesis , Abietanes/pharmacology , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/metabolism , Caspases/metabolism , Cell Cycle/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , G2 Phase Cell Cycle Checkpoints/drug effects , Humans , Inhibitory Concentration 50 , Liver Neoplasms/genetics , Liver Neoplasms/metabolism
3.
Pak J Pharm Sci ; 27(6 Suppl): 2013-8, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25410065

ABSTRACT

Synthesis of twelve hitherto unreported esters of abietyl alcohol and screening of these esters against four cancer cell lines including one breast cancer line MCF7 and four hepatocellular carcinoma cell lines (HCC) Huh7, Hep3B, Snu449 and Plc has been determined using SRB assay. The Cell cycle progression showed changes in cellular behaviour after 48 and 72 hours in MCF7 and Huh7 cell lines. Abietyl alcohol was obtained from the reduction of abietic acid, a tricyclic diterpene, isolated from oleoresin of Pinus longifolia Roxberghii.


Subject(s)
Breast Neoplasms/drug therapy , Carcinoma, Hepatocellular/drug therapy , Drug Screening Assays, Antitumor , Liver Neoplasms/drug therapy , Abietanes/pharmacology , Breast Neoplasms/pathology , Carcinoma, Hepatocellular/pathology , Cell Cycle/drug effects , Cell Line, Tumor , Fatty Acids/pharmacology , Humans , Liver Neoplasms/pathology , MCF-7 Cells
4.
Pak J Pharm Sci ; 27(5 Spec no): 1491-6, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25176231

ABSTRACT

Oleanolic acid (3ß-hydroxy-olean-12-en-28-oic acid; OA-01), a pentacyclic triterpene, exhibit a wide range of pharmacological and biological activities. We have isolated oleanolic acid from methanolic extract of Periploca aphylla, collected from surroundings of Karachi in the month of February. Furthermore, four known and two new C-28 amino acid conjugates of oleanolic acid were prepared to explore potential of these compounds on HCCs and one breast cancer cell line. Cytotoxic effects revealed that as compare to parent compound (OA-01), two derivatives OA-04 (p<0.0001) and OA-06 (p<0.01) showed significantly increased/higher inhibition rates.


Subject(s)
Amino Acids/chemical synthesis , Amino Acids/pharmacology , Antineoplastic Agents, Phytogenic/chemical synthesis , Antineoplastic Agents, Phytogenic/pharmacology , Carcinoma, Hepatocellular/pathology , Liver Neoplasms/pathology , Oleanolic Acid/chemical synthesis , Oleanolic Acid/pharmacology , Periploca , Breast Neoplasms/pathology , Cell Survival/drug effects , Dose-Response Relationship, Drug , Female , Hep G2 Cells , Humans , Inhibitory Concentration 50 , MCF-7 Cells , Oleanolic Acid/analogs & derivatives , Phytotherapy , Plant Bark , Plant Leaves , Plants, Medicinal
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