[EEG spectral characteristics in the dynamics of planned movements].

The spectral characteristics of the EEG in 11 healthy right-handed volunteers with no neurological disorders in dynamics of the unusual finger movements of both hands in random rhythm have been investigated. In particular, it is shown that the preparation and execution of voluntary finger movements compared to the rest were accompanied by a reduction level of activation within almost all the surface of cerebral cortex, except for right frontal lobe. It has been experimentally demonstrated that a significant increasing EEG activity of the right frontal area in high-frequency domain power spectrum was independent of the working limb. This might testify the direct participation of this cortical area in the processes of voluntary muscular activity planning, initiation and control.

[Electrographic correlates of real and imaginary movements: spectral analysis].

The research carried out with eight virtually healthy volunteers showed that movements were associated with the increased level of activation, primarily, in the central areas of the brain cortex, which was expressed in a reduction of the spectral power of alpha end beta frequencies and increase in the power of the gamma oscillations more expressed in the parietooccipital derivations of the left hemisphere. Mental reproduction of similar movements was accompanied by additional activation in the frontal, temporal, parietal, and occipitals regions and more pronounced increase in the power of the gamma oscillations. A number of electrographic phenomena associated with specific features of the movements were revealed.

[Individual resistance to factors of monotony reflected in the EEG].

Three groups of operators substantially different in their performance quality were examined. Under conditions of monotonous activity, subjects with the highest initial level of activation, minimum EEG total spectral power, and minimum level of EEG coherence in the frontal cortical areas worked most steadily. Under the same conditions, subjects with a rather high spectral power of the theta and beta2 EEG frequency components, highest coherence in the frontal areas, and low coherence in the caudal areas of the cortex worked least steadily. EEG phenomena testify to a rather low level of activation of the frontal cortical areas associated with a facilitation of cortico-subcortical neuronal interactions and an attenuation of the operating neural streams. This results in a decrease in the level of any attention, its involuntary switching, and short-term loss of the control over the current performance.

[The general laws of formation of a condition monotony].

In article results of research of spectral characteristics and a level of coherence basic rhythms EEG being under inspection of 19 with use are of resulted multifactorial dispersive analyses surveyed. On the basis of the received material an assumption comes out, that realization of base conditions of wakefulness is provided by own "built-in" regulating with mechanisms. Absence of such "built-in" mechanisms for a condition of monotonous causes "search" character of activity of regulating systems which results in the expressed periodicity in changes both a functional condition of a brain, and quality of activity carried out by the person.

[Current issues in municipal health services]. / Aktual'nye voprosy razvitiia munitsipal'nogo zdravookhraneniia.

A study, held in the Krasnodar Territory in 1994-2002, showed an increasing morbidity in residents of all age-categories. Essential differences in the prevalence of registered pathologies and in the nature of their dynamics were registered in some districts. An optimized structure of hospital beds resulted, in the above Territory, in a reduced quantity of beds, primarily in rural areas, and in their more effective utilization. More rural citizens applied for medical care to urban and territorial patient-care facilities, by 1.5 and 1.4 times respectively. Such reduction of hospital beds is possible only after advancing appropriately the regular medical check-ups and clinical care and after diminishing the need in the treatment of patients at hospitals. The data of sociological questioning of residents and of doctors held in three municipal entities by using the method of monitoring are presented. It was demonstrated that promotion of inter-district diagnostic centers, priority development of regular medical check-ups and purpose-oriented measures of reprofiling the specialized bed funds are topical issues in promoting the municipal medical care.

[Changes in characteristics of sensory evoked potentials in dynamics of monotonous activity]. / Izmenenie kharakteristik sensornykh vyzvannykh potentsialov v dinamike monotonnoi deiatel'nosti.

Visual and auditory evoked potentials were recorded in 19 volunteers during their monotonous activity. Three groups of subjects different in performance quality were set aside. Characteristics of evoked potential components were subjected to analysis of variance. The latency of P1 component of visual and auditory evoked potentials recorded in respective projection cortical areas significantly increased over the course of the long-term monotonous activity. These changes were associated with performance deterioration. The observed changes in performance quality and brain functional state were determined by individual features of subjects.

[Study of the nature of determinants for synthesis of delta-endotoxin and beta-exotoxin in Bacillus thuringiensis subsp. thuringiensis strains--bitoxybacillin producers]. / Izuchenie prirody determinant sinteza delta-éndotoksina i beta- ékzotoksina v shtammakh Bacillus thuringiensis subsp. thuringiensis--produtsentakh bitoksibatsillina.

The growth of Bacillus thuringiensis subsp. thuringiensis strains (BT) 202, 1140, 98 producing bitoxybacillin in the presence of novobiocin, mitomycin C and at high temperature 43 degrees C resulted in obtaining of the mutants for the synthesis of crystalline protein (Cry) and exotoxin (Exo). Analysis of the plasmid content of the mutants has shown the Cry Exo phenotyre to correlate with the loss of 55 MD plasmid in the strain BT202 and the loss of 60MD plasmid in BT1140. The transfer of these plasmids into Bacillus cereus leads to the transfer of endo- and exotoxin production properties. The synthesis of Cry in BT98 is controlled by the 56MD plasmid, while the synthesis of Exo is encoded by the chromosome or plasmid that cannot be eliminated or transferred into other strains. Localization of Cry on the 55 and 56 MD plasmids in BT202 and BT98 is confirmed by the hybridization of the plasmid DNA with the DNA-probe.

[Expression of delta-endotoxin gene from Bacillus thuringiensis var. berliner 1715 in Escherichia coli and Bacillus megaterium cells]. / Proiavlenie gena delta-éndotoksina Bacillus thuringiensis var. berliner 1715 v kletkakh Escherichia coli i Bacillus megaterium.

Effects of the structure of plasmids carrying the cloned delta-endotoxin gene (tox) ot Bacillus thuringiensis and of the culture media on the expression of the gene have been studied. The DNA region located upstream from the crystal protein gene promoter inhibited the expression of the tox gene in Escherichia coli cells, but enhanced the expression in Bacillus megaterium cells grown in LB medium. The upstream DNA region did not affect the tox gene expression when Bacillus megaterium cells were grown in SSM medium.