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Repressor mutant forms of the Azospirillum brasilense NtrC protein.

Huergo, Luciano F; Assumpção, Marcelo C; Souza, Emanuel M; Steffens, M Berenice R; Yates, M Geoffrey; Chubatsu, Leda S; Pedrosa, Fábio O.

Appl Environ Microbiol ; 70(10): 6320-3, 2004 Oct.

Article in English | MEDLINE | ID: mdl-15466584

ABSTRACT

The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.

Subject(s)

Azospirillum brasilense/genetics , Azospirillum brasilense/metabolism , Bacterial Proteins/genetics , Amino Acid Sequence , Azospirillum brasilense/drug effects , Bacterial Proteins/metabolism , Base Sequence , DNA, Bacterial/genetics , Genes, Bacterial , Molecular Sequence Data , Mutation , Nitrates/metabolism , Nitrogen Fixation/genetics , Nitrogenase/genetics , Nitrogenase/metabolism , Nitrosoguanidines/pharmacology , Operon , PII Nitrogen Regulatory Proteins , Phenotype , Repressor Proteins/genetics , Repressor Proteins/metabolism , Sequence Homology, Amino Acid

ABSTRACT

Subject(s)

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