[Morphological study of lymphoid organs at early stages of experimental prostate carcinogenesis].

AIM: the aim of our study was to identify of structural transformations in the thymus and lymph nodes at the early stages of the development of an experimental prostate cancer. MATERIALS AND METHODS: Experiments were performed on 60 adult male CBA mice weighing 28-30 g. An experimental model of a prostate cancer was created by inoculating a diluted cell strain of Ehrlich Ascites Carcinoma cells into the prostatic parenchyma through midline laparotomy under ether anesthesia. The animals were divided into three groups of 20 each: 1) control (intact animals) 2) animals on the 5th day of experimental prostate tumor growth and 3) animals on the 18th day of experimental prostate tumor growth. The pathological examination of the prostate, thymus, and pelvic lymph nodes was performed. The selection of structural components and cellular elements in the thymus and lymph nodes was carried out according to the International histological nomenclature. RESULTS: According to the results, there was an increase in the percentage of connective tissue elements and glandular tissue increased in the thymus and the number of immunoblasts increased on the 5th day of the experiment. However, disorganization of the thymus structure was detected on the 18th day as well as an increase in the cortical substance, the number of epithelial reticular cells and decrease in the number of immunoblasts. On the 5th day, paracortical hyperplasia, sinus histiocytosis and activation of the blast transformation were detected in lymphatic nodes. On the 18th day, metastases, follicular reaction and activation of the transport function were determined. CONCLUSION: Our data suggest that the accidental thymus involution and impairment of transport and immune functions of pelvic lymph nodes in experimental prostate cancer. The severity of disturbances depends on the stage of prostate carcinogenesis.

Recombination of Correlations between Bioelements in the Liver and Lungs during Modeling of Diet-Induced Obesity.

In rats with obesity induced by high fat ration, the concentrations of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr were measured in the liver and lungs using synchrotron X-ray fluorescence analysis. Recombinations of inter-element correlations in the liver and lungs of rats receiving or not receiving additional Zn were observed under fasting conditions and after eating lard. Sr and Ca made the most pronounced contribution in the redistribution of correlations in the liver of obese rats. In contrast to other bioelements, Sr had negative correlations with all study elements in the liver of obese rats during physiological starvation. Long-term administration of Zn to rats was followed by recombination of both fasting and postprandial inert-element correlations in the liver and lungs, but Zn concentration did not change under these conditions. Redistribution of correlations in the liver and lungs of obese rats under fasting conditions reflects fundamental changes in metabolic processes, while redistribution observed after fat meal indicates temporary (postprandial) changes.

Effect of zinc on the content of chemical elements in the lung tissue during obesity in the experiment.

We found no deviations from normalcy in the content of chemical elements (K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr) in the lungs of rats with mild alimentary obesity, but revealed redistribution of correlations between the elements indicating impaired metabolism in this organ. Zinc supplementation had no effect on the body weight and content of chemical elements (including zinc) in the lung tissue in rats fed high fat diet, but led to significant changes in the correlations between the elements. Bromine, rubidium, and strontium are actively involved in interelement interactions in the lung tissue. These elements should be given more attention in considering biological processes including alimentary obesity.

Thymus in experimental carcinogenesis of the prostate gland.

We studied structural changes in the prostate gland, thymus, and lymph nodes in CBA mice after transplantation of Ehrlich ascites tumor cells into the prostate gland. On experimental day 5, the number of blood and lymph vessels decreased in the gland; the percentage of connective tissue elements and glandular tissue and the number of immunoblasts in the thymus increased. On day 18, the number of blood vessels in the tumor decreased; the width of the cortex and glandular tissue increased in the thymus, while the number of immunoblasts was reduced. On day 28, tumor infiltration and increased number of lymphatic vessels in its stroma were observed; parenchyma was reduced, and the area of the connective tissue increased in the thymus. These structural changes indicated the development of accidental involution of the thymus during carcinogenesis of the prostate.

Effects of zinc on the content of chemical elements in the liver of rats during early stages of obesity.

In rats with slight alimentary obesity, the content of chemical elements in the liver did not differ from normal, but the correlations between these elements were changed, which attested to metabolic disorders. Additional zinc dose administered to rats receiving lipid-rich rations did not affect animal body weights and content of chemical elements (including zinc) in the liver, but corrected correlations between the elements. Active contribution of strontium, bromine, and rubidium to interactions between the elements in the liver necessitates studies of their role in biological processes, specifically, in initiation and development of obesity.

Micronutrients and macronutrients in the liver in experimental alimentary obesity.

Strong and very strong positive correlations differing from those in the control were found between chemical elements in the liver of rats with experimental alimentary obesity under conditions of physiological hunger and soon after fatty food intake. These findings attest to sustained metabolism disturbances in obesity and formation of a pathological system. Considering the appearance of strong and very strong correlations of Br and Rb in alimentary obesity, more attention should be paid to the investigation of the role of these poorly studied elements in lipid metabolism.

[Essential elements (Fe, Cu, Mn, Zn, Se, As) in the liver and lungs with alimentary obesity in the experiment].

The purpose - to establish the role of lungs in the metabolic processes in normal and alimentary obesity. The method of X-ray fluorescence analysis using synchrotron radiation, the determination of concentrations Fe, Cu, Mn, Zn, Se, As in the tissue of the lungs and liver in 19 control rats and 19 rats with alimentary obesity. On the basis of many strengths and very strong positive correlation between the content of chemical elements shows that in the control rats, fasting metabolism in the lung more intense than the liver, which in case of obesity is significantly reduced. After receiving the lard in the lungs decreases the number of correlations between the concentrations of microelements in the control animals. Obesity changing their character, indicating a switch of metabolism in connection with the utilization of triglycerides in the body of the control rats, and a malfunction of this process in obesity.

[Role of the lungs in utilization of triglycerides from blood plasma after administration of fat in norm and experimental obesity].

The experiment was carried out on 72 rats (36 control and 36 with alimentary adiposity). There were established the absence of distinctions in concentration of triglyceride in plasma of blood from the right auricle and a carotid at rats with adiposity in 2 hours after the unitary use of lard in comparison with control rats. Plasma of blood from the right auricle of control animals contained more triglyceride and this facts indirectly testifies to decrease of their recycling in lungs at adiposity.

[Impact of purified drinking water use on the small bowel lymphatic region].

The structures of the mesenteric lymph nodes, small bowel, microvascular bed, and a mast cell population of the mesentery of rats were studied by light microscopy. Tap water, filtered water, and macro- and microelement-balanced water were used. The use of the filtered and macro- and micro-element water caused increases in lymph transport in the mesenteric lymph nodes and in the height of small intestinal villi, activated humoral immunity, and produced the most optimum level of a mast cell population and the structure of the mesenteric microvascular bed.

Hemolymphomicrocirculatory bed of the pancreas during acute experimental pancreatitis.

Histochemical study of rat pancreas was carried out on days 14 and 30 of acute pancreatitis. The area of the stroma increased after damage and the area of lymph microcirculatory component increased in comparison with the blood circulation component.

[Sanatorium rehabilitation of patients with chronic prostatitis].

A total of 75 males from married couples with circulatory prostatitis aged 19-47 were examined and treated in Barnaulsky sanatorium. The patients were trained by walking with the load power 80-110 W for 2.5 to 3 hours. They also received phytocorrection with biologically active food additive Fitopan M. The treatment led to attenuation of pelvic symptoms, stabilization of morning erection, normalization of friction period and laboratory indices. The effect persisted for 3 years.

[The structural organization of the regional lymph nodes of the heart in the acute period of experimental myocardial ischemia]. / Strukturnaia organizatsiia regionarnykh limfaticheskikh uzlov serdtsa v ostrom periode éksperimental'noi ishemii miokarda.

Anterior mediastinal and popliteal lymph nodes were studied morphologically and cytologically in male Wistar line rats in an acute period of experimental ischemia resulting from ligation of the left coronary artery. Three stages of structural-functional changes were demonstrated. In first stage increase of cortical substance square, widening of intermediate sinuses and macrophage number increase in all lymph node zones were observed in both groups after ligation of the artery. Second stage (day III of the ischemia) is characterized with the accumulation of plasmatic series cells and reaction of blast transformation in lymph node zones. In third stage (day 7 of the ischemia) restoration of medullar sinuses square up to the control values and even certain increase of the latter were observed. Morphofunctional changes in an acute period of the experimental ischemia of myocardium depend on the lymph node functional specialization and they are greater expressed in mediastinal lymph nodes of the heart region. It was suggested that in an acute period of ischemia of myocardium lymph nodes influence the quantity and cellular content of the passing lymph.

[Study of blood and lymph in experimental myocardial ischemia and arterial hypertension]. / Issledovanie krovi i limfy pri éksperimental'noi ishemii miokarda i arterial'noi gipertenzii.

The peripheral blood and central lymph of rats under experimental myocardial infarction was studied by means of light microscopy and electric conductivity measurement. Both hypertensive rats and animals 3 days after myocardial infarction had similar quantity of neutrophils in peripheral blood. Lymph cells count of hypertensive rats by middle lymphocytes is similar to the animals 1 day after myocardial infarction. The correlation between lymph and blood electric conductivity and its cell composition was noted.

[Blood flow in the popliteal lymph node region of rats in experimental myocardial ischemia and its treatment with dog-rose polyphenols]. / Ob"emnyi krovotok v oblasti podkolennogo limfaticheskogo uzla krysy pri éksperimental'noi ishemii miokarda i korrektsii preparatom polifenolov shipovnika.

The regional blood flow in the distal part of the rat great adductor muscle was measured using polarographic method in the norm, under the condition of experimental myocardial infarction and after treatment with dog-rose polyphenols preparation. Maximal decrease of the regional blood flow was detected on the third day after the operation and it was recovered on the seventh day. The treatment led to normalization of the regional blood flow.