1.
Eur J Biochem
; 155(2): 447-52, 1986 Mar 03.
Article
in English
| MEDLINE
| ID: mdl-3956495
ABSTRACT
Isolated rat hepatocytes were cultured in monolayer for about 24 h. During this period the cells exhibited constant protein and lipid synthesis. When the culture medium contained compactin, a competitive inhibitor of the 3-hydroxyl-3-methylglutary-coenzyme-A reductase, dolichyl-P synthesis was inhibited by 91% at the end of the incubation, as estimated by the incorporation of [3H]acetate and by 77% as estimated by the incorporation of 32Pi. These results indicate that in primary cultures of rat hepatocytes dolichyl monophosphate is mainly synthesized through a de novo process, while phosphorylation through the CTP-mediated kinase is of limited functional importance.
Subject(s)
Dolichol Phosphates/biosynthesis , Liver/metabolism , Lovastatin/analogs & derivatives , Naphthalenes/pharmacology , Polyisoprenyl Phosphates/biosynthesis , Acetates/metabolism , Acetic Acid , Animals , Cells, Cultured , Cholesterol/biosynthesis , Cytidine Triphosphate/physiology , Glycerol/metabolism , Leucine/metabolism , Liver/drug effects , Male , Phospholipids/biosynthesis , Phosphorus Radioisotopes , Phosphorylation , Protein Biosynthesis , Rats , Tritium
2.
Acta Chem Scand B
; 37(3): 244-6, 1983.
Article
in English
| MEDLINE
| ID: mdl-6414204
Subject(s)
Carbonic Anhydrases/blood , Cobalt , Erythrocytes/enzymology , Guanidines/pharmacology , Animals , Cattle , Enzyme Activation , Guanidine , Humans , Kinetics , Protein Denaturation/drug effects
3.
Acta Chem Scand B
; 37(3): 261-2, 1983.
Article
in English
| MEDLINE
| ID: mdl-6624361