Clinical and Optical Coherence Tomography Analysis of Intraretinal Microcysts in Patients with Epiretinal Membrane.

Purpose: To perform clinical and optical coherence tomography analysis of intraretinal microcysts (IRM) in patients undergoing pars plana vitrectomy (PPV) for primary epiretinal membrane (ERM) treatment.Materials and Methods: The files of 137 patients who were operated on by a single surgeon for primary epiretinal membrane treatment in our clinic between September 2017 and January 2020 were retrospectively reviewed. Patients with ERM due to secondary pathology were excluded from the study. Twenty patients with post-operative IRM (group 1) were determined and were included as the study group. To determine the effect of IRMs on visual acuity 20 patients who did not have IRM, had primary ERM and underwent PPV for treatment were included as the control group (group 2). The demographic, clinical, and operative data of the patients were obtained from the hospital database. Retinal analysis of the patients before the operation and at the earliest 6 months after the operation was performed using 6 × 6 mm images taken with the macular cube 512 × 128 protocol on the Cirrus HD-OCT (Carl Zeiss Meditec, Dublin, CA, USA) device in our clinic.Results: Intraretinal microcysts were present in 8 (5.8%) of preoperative 137 patients and 20 (14.6%) of 137 postoperative patients. All 8 patients with preoperative cysts in group 1 also had postoperative IRMs. Excluding patients with preoperative cysts, the postoperative IRM incidence was found to be 12/137 (8.75%). Preoperative (p = .392) and postoperative (p = .978) visions were similar in group 1 and group 2. Preoperative (p = .745) and postoperative retinal thicknesses were similar in both groups (p = .989). The incidence of postoperative (p = .642) IRM was similar in patients who underwent ERM and ERM + internal limiting membrane (ILM) peeling in group 1.Conclusions: Intraretinal microcysts may sometimes be seen before and after ERM surgery. The preoperative cyst presence in ERM patients is an important risk factor for postoperative cyst presence. The peeling of ILM with ERM does not increase the probability of IRM occurrence. The presence of these cysts before and/or after the operation does not affect the visual prognosis and macular thickness in ERM patients.

Callus induction and plant regeneration of the endemic Astragalus nezaketae in Turkey

A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [a-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100 percent) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite.

Mechanisms of boron tolerance and accumulation in plants: a physiological comparison of the extremely boron-tolerant plant species, Puccinellia distans, with the moderately boron-tolerant Gypsophila arrostil.

The physiological characteristics of the extremely boron (B)-tolerant plant species, Puccinellia distans, were compared with those of the moderately tolerant Gypsophila arrostil, two species collected from a B-mining area of Eskisehir, Turkey. Boron was supplied to plants hydroponically at B concentrations ranging from 0.5 to 50 mg B/L for G. arrostil, and from 0.5 to 2000 mg B/L for P. distans. The results show that P. distans has a strikingly greater tolerance to B than G. arrostil. While G. arrostil was unable to survive B supply concentrations greater than 50 mg B/L, P. distans grew at B supply concentrations exceeding 1250 mg B/L. Our research supports the conclusion that from 0.5 to 50 mg B/L, P. distans is better able to restrict the accumulation of B in the whole plant, and the transport of B from root to shoot, than G. arrostil. We propose that P. distans uses several strategies to achieve B tolerance including the ability to restrict the accumulation of B relative to its accumulation of biomass, the ability to restrict the transport of B from root to shoot, and, to a lesser extent, the ability to tolerate high concentrations of B in its shoot and root tissues.

Determination of minerals extracted from several commercial teas (Camellia sinensis) to hot water (infusion).

Mineral contents of some tea and their infusions drunk in Turkey were established by inductively coupled plasma atomic emission spectrometry. The Al, Ca, K, Mg, Mn, P, and S contents were very high in both infusions and tea (i.e., pieces of the tea plant). The As, Cd, Cr, Li, Pb, and Se contents of infusion and tea were found to be very low. The level of K of all samples is higher than those of other minerals. Generally, mineral contents of tea were found to be higher than those of tea infusions. In addition, the health benefits of teas and knowledge of their mineral contents are of great interest and may be useful for further study of enzyme systems and vital biochemical functions.