ABSTRACT
Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR.
Subject(s)
Arthrodermataceae/genetics , DNA Fingerprinting/methods , DNA, Fungal/genetics , Dermatomycoses/epidemiology , Genotype , Trichophyton/genetics , Adolescent , Adult , Aged , Arthrodermataceae/classification , Arthrodermataceae/isolation & purification , Child , Dermatomycoses/diagnosis , Dermatomycoses/microbiology , Female , Humans , Male , Middle Aged , Polymerase Chain Reaction/methods , Sequence Analysis, DNA , Trichophyton/classification , Turkey/epidemiology , Young AdultABSTRACT
OBJECTIVE: To investigate the distribution of hepatitis B virus (HBV) genotypes among patients with chronic hepatitis B in Kayseri, Turkey. METHODS: The study took place in the Department of Microbiology, Erciyes University, Kayseri, and Iontek Laboratory, Istanbul, Turkey, from January 2005 to October 2007. One hundred and ten patients with chronic hepatitis B were included in this study. Hepatitis B virus DNA in sera were investigated by using the real-time polymerase chain reaction. Viral DNA was extracted from 200 uL of serum using the QIAamp DNA minElute kit (Qiagen, Hilden, Germany). Reaction mixture was prepared by Fluorion HBV QNP 2.0 ( Iontek, Istanbul, Turkey). RESULTS: Genotype D was detected in 107 of 110 (97.2%) patients, however, genotyping failed in 3 patients (2.7%). No other genotypes were found. CONCLUSION: The vast majority of Turkish patients with chronic hepatitis B have genotype D.
