Subject(s)
Cholera Toxin/isolation & purification , Dermotoxins/isolation & purification , Vibrio cholerae/pathogenicity , Vibrio/pathogenicity , Animals , Cholera Toxin/toxicity , Dermotoxins/toxicity , Humans , Mice , Rabbits , Skin/drug effects , Vibrio/isolation & purification , Vibrio cholerae/isolation & purification , Vibrio parahaemolyticus/isolation & purification , Vibrio parahaemolyticus/pathogenicity , VirulenceABSTRACT
The dermonecrotic factor (dermotoxin) inducing skin necrosis in rabbits has been isolated from V. cholerae strain B-53-2-38 and partially purified. Dermotoxin has a molecular weight of about 110 kD and possesses pronounced cytotoxic and general toxic action, differing from that of enterotoxin. The introduction of this factor into the blood and peritoneum of laboratory animals causes their death.
Subject(s)
Cholera Toxin/isolation & purification , Dermotoxins/isolation & purification , Vibrio cholerae/pathogenicity , Animals , Animals, Suckling , Bacteriological Techniques , Cholera Toxin/toxicity , Dermotoxins/toxicity , Guinea Pigs , Mice , Molecular Weight , Rabbits , Skin/drug effectsABSTRACT
Y. pestis cells have been shown capable of binding fibronectin (FN), the presence of adhesion pili considerably enhancing FN binding. The study has established that, along with FN, native adhesive pili, but not subunits, are capable of binding mucin and ganglioside. Structures similar to FN-binding curlings of Escherichia have been found on the surface of Y. pestis cells. The expression of curling-like structures does not depend on the presence of plasmids in Y. pestis cells.
Subject(s)
Fibronectins/metabolism , Yersinia pestis/metabolism , Animals , Bacterial Adhesion/physiology , Cattle , Electrophoresis, Polyacrylamide Gel , Fibronectins/analysis , Fibronectins/ultrastructure , Fimbriae, Bacterial/metabolism , Fimbriae, Bacterial/ultrastructure , Microscopy, Electron , Protein Binding , Yersinia pestis/pathogenicity , Yersinia pestis/ultrastructureSubject(s)
DNA-Directed RNA Polymerases/analysis , Temperature , Yersinia pestis/enzymology , Chemical Phenomena , Chemistry, Physical , DNA, Bacterial/genetics , DNA-Directed RNA Polymerases/isolation & purification , Electrophoresis, Gel, Two-Dimensional , Escherichia coli/enzymology , Microscopy, Electron , Phosphorylation , Plasmids , Transcription, Genetic , Yersinia pestis/genetics , Yersinia pestis/ultrastructureSubject(s)
DNA-Directed RNA Polymerases/isolation & purification , Yersinia pestis/enzymology , Chromatography, Gel , DNA/genetics , DNA-Directed RNA Polymerases/analysis , DNA-Directed RNA Polymerases/antagonists & inhibitors , DNA-Directed RNA Polymerases/genetics , Electrophoresis, Polyacrylamide Gel , Enzyme Stability , Substrate Specificity , Transcription, Genetic , Yersinia pestis/geneticsABSTRACT
Proteinograms of 112 strains of vibrios and closely affiliated microorganisms were studied by disc electrophoresis in polyacrylamide gel. Up to 25 protein peaks with definite mobility coefficients were revealed. The influence of the culture medium on the protein spectrum of the microbes was found. The frequency of peak formation was of great significance for the differentiation of the microbes under study. The quantitative characteristics of the peak area could not be used for differentiation.
Subject(s)
Bacterial Proteins/analysis , Electrophoresis, Polyacrylamide Gel/methods , Vibrio/analysis , Aeromonas/analysis , Hydrogen-Ion Concentration , Pseudomonas/analysis , Solubility , Vibrio cholerae/analysisABSTRACT
The authors present the results of a comparative study of the protein spectra of 80 strains of various vibrios. Proteinograms were elaborated by the method of disc-electrophoresis in polyacrylamide gel for each strain and species. There were revealed 25 protein peaks with definite mobility coefficients. The number of peaks and their areas varied in different species. The qualitative and the quantitative differences between the vibrio species were established. Results of investigations demonstrated a possibliity of using the protein spectra for the differentiation of microorganisms.