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1.
Sci Rep ; 8(1): 17566, 2018 12 04.
Article in English | MEDLINE | ID: mdl-30514885

ABSTRACT

Galactofuranosyltransferases are poorly described enzymes despite their crucial role in the virulence and the pathogenicity of numerous microorganisms. These enzymes are considered as potential targets for therapeutic action. In addition to the only well-characterised prokaryotic GlfT2 from Mycobacterium tuberculosis, four putative genes in Leishmania major were previously described as potential galactofuranosyltransferases. In this study, we have cloned, over-expressed, purified and fully determined the kinetic parameters of these four eukaryotic enzymes, thus demonstrating their unique potency in catalysing the transfer of the galactofuranosyl moiety into acceptors. Their individual promiscuity revealed to be different, as some of them could efficiently use NDP-pyranoses as donor substrates in addition to the natural UDP-galactofuranose. Such results pave the way for the development of chemoenzymatic synthesis of furanosyl-containing glycoconjugates as well as the design of improved drugs against leishmaniasis.


Subject(s)
Galactose/analogs & derivatives , Galactosyltransferases/biosynthesis , Galactosyltransferases/chemistry , Leishmania major/enzymology , Protozoan Proteins/biosynthesis , Protozoan Proteins/chemistry , Uridine Diphosphate/analogs & derivatives , Biocatalysis , Escherichia coli/genetics , Galactose/metabolism , Kinetics , Substrate Specificity , Uridine Diphosphate/metabolism
2.
Beilstein J Org Chem ; 13: 1857-1865, 2017.
Article in English | MEDLINE | ID: mdl-29062404

ABSTRACT

Carbohydrate related enzymes, like glycosyltransferases and glycoside hydrolases, are nowadays more easily accessible and are thought to represent powerful and greener alternatives to conventional chemical glycosylation procedures. The knowledge of their corresponding mechanisms has already allowed the development of efficient biocatalysed syntheses of complex O-glycosides. These enzymes can also now be applied to the formation of rare or unnatural glycosidic linkages.

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