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2.
J Obstet Gynecol Neonatal Nurs ; 52(1): 84-94, 2023 01.
Article in English | MEDLINE | ID: mdl-36183744

ABSTRACT

OBJECTIVE: To describe the frequency and severity of traumatic childbirth events (TCEs) and how they affected the professional practice and personal lives of maternity care clinicians, including registered nurses (RNs), certified nurse-midwives, attending physicians, and resident physicians. DESIGN: Descriptive cross-sectional study. SETTING: Maternity units across five hospitals in the Baltimore metropolitan area. PARTICIPANTS: Maternity care clinicians (N = 160) including RNs (n = 104), certified nurse-midwives (n = 17), attending physicians (n = 28), and resident physicians (n = 11). METHODS: Participants completed an online survey to measure the frequency and severity of TCEs and how they affect participants' professional practice and personal lives. We used descriptive statistics to characterize maternity care clinicians and bivariate analysis and linear regression to examine relationships. RESULTS: Most participants were women (92.5%), White (62.5%), between the ages of 21 and 54 years (89.4%), RNs (65.0%), and employed full-time (79.2%). Shoulder dystocia was the most frequently observed TCE (90.6%), and maternal death was the most severe TCE (M = 4.82, SD = 0.54). Attending physicians (50.0%) reported a significantly greater frequency of exposure to TCEs than the other participants, χ2(6) = 23.8 (n = 159), p <. 001. The frequency of TCEs had a significant medium correlation with perceived effect on professional practice, r(154) = 0.415, p < .001, and personal life, r(155) = 0.386, p < .001. Perception of severity was strongly associated with professional practice, ß = 0.52, p < .001, and personal life, ß = 0.46, p < .001. CONCLUSION: If severe, TCE exposure can affect the professional practice and personal life of maternity care clinicians.


Subject(s)
Maternal Health Services , Midwifery , Nurse Midwives , Obstetrics , Female , Pregnancy , Humans , Young Adult , Adult , Middle Aged , Male , Cross-Sectional Studies , Attitude of Health Personnel
3.
JAMA Netw Open ; 5(11): e2244077, 2022 11 01.
Article in English | MEDLINE | ID: mdl-36445707

ABSTRACT

Importance: In the US, more than 50 000 women experience severe maternal morbidity (SMM) each year, and the SMM rate more than doubled during the past 25 years. In response, professional organizations called for birthing facilities to routinely identify and review SMM events and identify prevention opportunities. Objective: To examine SMM levels, primary causes, and factors associated with the preventability of SMM using Maryland's SMM surveillance and review program. Design, Setting, and Participants: This cross-sectional study included pregnant and postpartum patients at 42 days or less after delivery who were hospitalized at 1 of 6 birthing hospitals in Maryland between August 1, 2020, and November 30, 2021. Hospital-based SMM surveillance was conducted through a detailed review of medical records. Exposures: Hospitalization during pregnancy or within 42 days post partum. Main Outcomes and Measures: The main outcomes were admission to an intensive care unit, having at least 4 U of red blood cells transfused, and/or having COVID-19 infection requiring inpatient hospital care. Results: A total of 192 SMM events were identified and reviewed. Patients with SMM had a mean [SD] age of 31 [6.49] years; 9 [4.7%] were Asian, 27 [14.1%] were Hispanic, 83 [43.2%] were non-Hispanic Black, and 68 [35.4%] were non-Hispanic White. Obstetric hemorrhage was the leading primary cause of SMM (83 [43.2%]), followed by COVID-19 infection (57 [29.7%]) and hypertensive disorders of pregnancy (17 [8.9%]). The SMM rate was highest among Hispanic patients (154.9 per 10 000 deliveries), primarily driven by COVID-19 infection. The rate of SMM among non-Hispanic Black patients was nearly 50% higher than for non-Hispanic White patients (119.9 vs 65.7 per 10 000 deliveries). The SMM outcome assessed could have been prevented in 61 events (31.8%). Clinician-level factors and interventions in the antepartum period were most frequently cited as potentially altering the SMM outcome. Practices that were performed well most often pertained to hospitals' readiness and adequate response to managing pregnancy complications. Recommendations for care improvement focused mainly on timely recognition and rapid response to such. Conclusions and Relevance: The findings of this cross-sectional study, which used hospital-based SMM surveillance and review beyond the mere exploration of administrative data, offers opportunities for identifying valuable quality improvement strategies to reduce SMM. Immediate strategies to reduce SMM in Maryland should target its most common causes and address factors associated with preventability identified at individual hospitals.


Subject(s)
COVID-19 , Pregnancy , Humans , Female , Child , Maryland/epidemiology , COVID-19/epidemiology , Cross-Sectional Studies , Black People , Ethnicity
4.
Geophys Res Lett ; 46(5): 2984-2992, 2019 Mar 16.
Article in English | MEDLINE | ID: mdl-31217646

ABSTRACT

The NASA Cyclone Global Navigation Satellite System (CYGNSS) was launched in December 2016, providing an unprecedented opportunity to obtain ocean surface wind speeds including wind estimates over the hurricane inner-core region. This study demonstrates the influence of assimilating an early version of CYGNSS observations of ocean surface wind speeds on numerical simulations of two notable landfalling hurricanes, Harvey and Irma (2017). A research version of the National Centers for Environmental Prediction operational Hurricane Weather Research and Forecasting model and the Gridpoint Statistical Interpolation-based hybrid ensemble three-dimensional variational data assimilation system are used. It is found that the assimilation of CYGNSS data results in improved track, intensity, and structure forecasts for both hurricane cases, especially for the weak phase of a hurricane, implying potential benefits of using such data for future research and operational applications.

5.
Sensors (Basel) ; 18(12)2018 Dec 05.
Article in English | MEDLINE | ID: mdl-30563181

ABSTRACT

This study presents wind observations from an airborne Doppler Wind Lidar (ADWL) in 2016 tropical cyclones (TC). A description of ADWL measurement collection and quality control methods is introduced for the use in a TC environment. Validation against different instrumentation on-board the National Oceanographic and Atmospheric Administration's WP-3D aircraft shows good agreement of the retrieved ADWL measured wind speed and direction. Measurements taken from instruments such as the global positioning system dropsonde, flight-level wind probe, tail Doppler radar, and Stepped Frequency Microwave Radiometer are compared to ADWL observations by creating paired datasets. These paired observations represent independent measurements of the same observation space through a variety of mapping techniques that account for differences in measurement procedure. Despite high correlation values, outliers are identified and discussed in detail. The errors between paired observations appear to be caused by differences in the ability to capture various length scales, which directly relate to certain regions in a TC regime. In validating these datasets and providing evidence that shows the mitigation of gaps in 3-dimensional wind representation, the unique wind observations collected via ADWL have significant potential to impact numerical weather prediction of TCs.

6.
Matern Child Health J ; 21(2): 260-266, 2017 02.
Article in English | MEDLINE | ID: mdl-27461023

ABSTRACT

Purpose Mercy Medical Center (MMC), a community hospital in Baltimore Maryland, has undertaken a community initiative to reduce low birth weight (LBW) deliveries by 10 % in 3 years. MMC partnered with a School of Public Health to evaluate characteristics associated with LBW deliveries and formulate collaborations with obstetricians and community services to improve birth outcomes. Description As part of the initiative, a case control study of LBW was undertaken of all newborns weighing <2500 grams during June 2010-June 2011 matched 2:1 with newborns ≥2500 grams (n = 862). Assessment Logistic regression models including maternal characteristics prior to and during pregnancy showed an increased odds of LBW among women with a previous preterm birth (aOR 2.48; 95 % CI: 1.49-4.13), chronic hypertension (aOR: 2.53; 95 % CI: 1.25-5.10), hospitalization during pregnancy (aOR: 2.27; 95 % CI:1.52-3.40), multiple gestation (aOR:12.33; 95 % CI:5.49-27.73) and gestational hypertension (aOR: 2.81; 95 % CI: 1.79-4.41). Given that both maternal pre-existing conditions and those occurring during pregnancy were found to be associated with LBW, one strategy to address pregnant women at risk of LBW infants is to improve the intake and referral system to better triage women to appropriate services in the community. Meetings were held with community organizations and feedback was operationalized into collaboration strategies which can be jointly implemented. Conclusion Education sessions with providers about the referral system are one ongoing strategy to improve birth outcomes in Baltimore City, as well as provision of timely home visits by nurses to high-risk women.


Subject(s)
Hypertension/complications , Infant, Low Birth Weight/physiology , Patient Outcome Assessment , Public Health/methods , Adult , Baltimore/epidemiology , Case-Control Studies , Female , Humans , Hypertension/epidemiology , Hypertension, Pregnancy-Induced/epidemiology , Hypertension, Pregnancy-Induced/prevention & control , Infant , Infant Mortality , Infant, Low Birth Weight/metabolism , Infant, Newborn , Maternal Mortality , Pregnancy , Prenatal Care/methods , Prenatal Care/standards , Public-Private Sector Partnerships/standards
7.
Am J Obstet Gynecol ; 211(5): 514.e1-7, 2014 Nov.
Article in English | MEDLINE | ID: mdl-24746997

ABSTRACT

OBJECTIVE: To derive a prediction rule for preeclampsia and early onset preeclampsia requiring delivery <34 weeks using first trimester maternal, ultrasound, and serum markers. STUDY DESIGN: Prospective cohort study of women enrolled at first trimester screening. Maternal history, demographics, anthropometry, ultrasound parameters, and serum analytes were compared between women with preeclampsia and normal outcome. The prediction rule was derived by Lasso logistic regression analysis. RESULTS: In 2441 women, 108 (4.4%) women developed preeclampsia, and 18 (0.7%) early preeclampsia. Nulliparity, prior hypertension, diabetes, prior preeclampsia, mean arterial pressure, and the log pregnancy-associate pregnancy protein-A multiples of the median were primary risk factors. Prediction rules for preeclampsia/early preeclampsia had an area under the curve of 0.82/0.83 respectively. Preeclampsia was predicted with 49% sensitivity and early preeclampsia with 55% sensitivity for a 10% false positive rate. CONCLUSION: First trimester prediction rules using parameters currently available at first trimester screening identify a significant proportion of women with subsequent preeclampsia.


Subject(s)
Hypertension/epidemiology , Pre-Eclampsia/diagnosis , Pregnancy Complications, Cardiovascular/epidemiology , Pregnancy in Diabetics/epidemiology , Uterine Artery/diagnostic imaging , Adolescent , Adult , Area Under Curve , Biomarkers/blood , Blood Pressure , Cohort Studies , Female , Humans , Logistic Models , Parity , Pre-Eclampsia/blood , Pre-Eclampsia/diagnostic imaging , Pregnancy , Pregnancy Trimester, First/blood , Pregnancy-Associated Plasma Protein-A/metabolism , Prenatal Diagnosis , Prognosis , Prospective Studies , Risk Assessment , Ultrasonography, Prenatal , Young Adult
8.
Am J Obstet Gynecol ; 211(3): 261.e1-8, 2014 Sep.
Article in English | MEDLINE | ID: mdl-24631442

ABSTRACT

OBJECTIVE: First-trimester screening for subsequent delivery of a small-for-gestational-age (SGA) infant typically focuses on maternal risk factors and uterine artery (UtA) Doppler. Our aim is to test if incorporation of fetal umbilical artery (UA) and ductus venosus (DV) Doppler improves SGA prediction. STUDY DESIGN: Prospective screening study of singletons at 11-14 weeks. Maternal characteristics, serum concentrations of pregnancy-associated plasma protein-A (PAPP-A) and free ß-human chorionic gonadotropin are ascertained and UtA Doppler, UA, and DV Doppler studies are performed. These parameters are tested for their ability to predict subsequent delivery of a SGA infant. RESULTS: Among 2267 enrolled women, 191 (8.4%) deliver an SGA infant. At univariate analysis women with SGA neonates are younger, more frequently African-American (AA), nulliparous, more likely to smoke, have lower PAPP-A and free ß-human chorionic gonadotropin levels. They have a higher incidence of UtA Doppler bilateral notching, higher mean UtA Doppler-pulsatility index z-scores (P < .001) and UA pulsatility index z-scores (P = .03), but no significant difference in DV-pulsatility index z-scores or in the incidence of abnormal qualitative UA and DV patterns. Multivariate logistic regression analysis identifies nulliparity and AA ethnicity (P < .001), PAPP-A multiple of the median and bilateral notching (P < .05) as determinants of SGA infant. Predictive sensitivity was low; receiver operating characteristic curve analysis yields areas under the curve of 0.592 (95% confidence interval, 0.548-0.635) for the combination of UtA Doppler and UA pulsatility index z-scores. CONCLUSION: Delivery of a SGA infant is most frequent in nulliparous women of AA ethnicity. Despite the statistical association with UtA Doppler first-trimester SGA prediction is poor and not improved by the incorporation of fetal Doppler.


Subject(s)
Infant, Small for Gestational Age , Umbilical Arteries/physiology , Adult , Black or African American , Chorionic Gonadotropin, beta Subunit, Human/blood , Female , Humans , Infant, Newborn , Logistic Models , Pregnancy , Pregnancy Trimester, First , Pregnancy-Associated Plasma Protein-A/analysis , Prospective Studies
9.
Obstet Gynecol ; 123(3): 611-617, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24513777

ABSTRACT

OBJECTIVE: The residual risk of preeclampsia in high-risk women on aspirin prophylaxis exceeds that of low-risk populations, and this study aimed to identify first-trimester maternal characteristics associated with aspirin prophylaxis failure. METHODS: This is a nested cohort study of prospectively enrolled women with verified initiation of risk-indicated aspirin prophylaxis by 16 weeks of gestation. First-trimester maternal history, demographics, anthropometry, ultrasound parameters, and serum analytes were compared between women who developed preeclampsia and those who did not. Blood pressure measurements were classified as prehypertension or hypertension according to the Joint National Committee on Hypertension guidelines. Chi square, nonparametric, and logistic regression analyses were used to determine the contributors to preeclampsia development. RESULTS: Six hundred fourteen women prospectively enrolled at 9-14 weeks of gestation initiated aspirin by 16 weeks of gestation. The 59 (9.6%) women who developed preeclampsia were more likely to have chronic hypertension, diabetes, and obesity and had higher first-trimester blood pressure and lower serum pregnancy-associated plasma protein-A concentrations (all P<.05). Having first-trimester Joint National Committee on Hypertension prehypertension or hypertension was associated with a 2.18-fold increased risk of developing preeclampsia, whereas normotension was associated with a reduction of risk of 56%. CONCLUSION: Women who develop preeclampsia while taking aspirin prophylaxis are more likely to have elevated first-trimester blood pressures. Conversely, first-trimester normotension is associated with a reduced risk of preeclampsia.


Subject(s)
Aspirin/therapeutic use , Platelet Aggregation Inhibitors/therapeutic use , Pre-Eclampsia/prevention & control , Adolescent , Adult , Blood Pressure , Body Mass Index , Decision Support Techniques , Drug Administration Schedule , Female , Follow-Up Studies , Humans , Logistic Models , Middle Aged , Pre-Eclampsia/diagnosis , Pre-Eclampsia/etiology , Pregnancy , Pregnancy Trimester, First , Prenatal Care , Prospective Studies , Risk Factors , Treatment Failure , Young Adult
10.
Hypertens Pregnancy ; 33(2): 204-14, 2014 May.
Article in English | MEDLINE | ID: mdl-24304164

ABSTRACT

OBJECTIVE: To evaluate the impact of prior preeclampsia on first trimester assessment in subsequent pregnancy. METHODS: A total of 1283 parous patients were prospectively enrolled at 9-14 weeks of gestation. Maternal biophysical characteristics, ultrasound parameters and placental analytes were compared between women with and without prior preeclampsia. RESULTS: There is no association between prior preeclampsia and the first trimester ultrasound parameters or placental analytes studied. The effects of prior preeclampsia in subsequent pregnancy are exaggerated by increasing parity and are predominantly blood pressure-related, independent of other cardiovascular risk factors. CONCLUSION: There is a potential role for lifestyle modification and stricter pregnancy blood pressure control in patients with prior preeclampsia.


Subject(s)
Chorionic Gonadotropin, beta Subunit, Human/blood , Pre-Eclampsia/physiopathology , Pregnancy Trimester, First/physiology , Pregnancy-Associated Plasma Protein-A/metabolism , Uterine Artery/physiopathology , Adolescent , Adult , Biomarkers/blood , Blood Pressure , Female , Humans , Middle Aged , Pre-Eclampsia/blood , Pre-Eclampsia/diagnostic imaging , Pregnancy , Prospective Studies , Ultrasonography , Uterine Artery/diagnostic imaging , Young Adult
11.
Arch Gynecol Obstet ; 283(3): 663-8, 2011 Mar.
Article in English | MEDLINE | ID: mdl-20419307

ABSTRACT

PURPOSE: To better characterize postpartum cerebral angiitis (PPCA). METHODS: We present a case of PPCA in which a 34-year-old G6P5104 underwent a normal vaginal delivery and developed PPCA. She had no signs or symptoms of gestational hypertension or preeclampsia. She had a history of migraines and received methylergonovine at delivery. She represented postpartum with headache and hypertension. The patient had characteristic findings of cerebral angiitis on imaging, and was diagnosed with PPCA. She was treated with nimodipine and steroids. She was monitored with transcranial Dopplers. RESULTS: In reviewing the literature, we found 23 cases of PPCA. We found that none had proteinuria, most were hypertensive, and all presented with headache. CONCLUSIONS: Use of sympathomimetic agents, particularly among those with migraines, may increase risk of PPCA.


Subject(s)
Pregnancy Complications/diagnosis , Vasculitis, Central Nervous System/diagnosis , Adult , Antihypertensive Agents/therapeutic use , Cerebral Angiography , Female , Humans , Hypertension/drug therapy , Migraine Disorders/drug therapy , Nimodipine/therapeutic use , Postpartum Period/drug effects , Pregnancy , Pregnancy Complications/drug therapy , Steroids/therapeutic use , Treatment Outcome , Vasculitis, Central Nervous System/drug therapy
12.
Am J Perinatol ; 27(1): 9-14, 2010 Jan.
Article in English | MEDLINE | ID: mdl-19504428

ABSTRACT

Angiopoietin-2 (Ang-2), synthesized by endothelial cells, is a marker of placental vascular remodeling. Ang-2 is expressed in the first trimester, and levels may therefore correlate to other parameters of placental vascular development. The aim of this study was to evaluate the relationships between Ang-2 and other maternal/placental factors in the first trimester. This was a prospective observational study of women presenting for first-trimester screening at 11 + 0 to 13 + 6 weeks. Consenting women underwent an ultrasound, physical examination, and blood draw. Maternal serum Ang-2 levels were determined using enzyme-linked immunosorbent assay. Results were evaluated with relation to maternal age, parity, race, body mass index (BMI), mean arterial pressure (MAP), smoking/caffeine use, and parameters of placental blood flow resistance. In 111 consecutive patients, serum Ang-2 ranged from 0.6 to 10.9 ng/mL. Ang-2 levels were unrelated to maternal age, race, parity, smoking, and caffeine intake. Significant negative correlations were observed with BMI (Pearson's R = -0.325; P < 0.0001) and MAP (Pearson's R = -0.287; P = 0.002). Ang-2 levels did not correlate with gestational age (Spearman's rho, 0.064; P = 0.5058), but a significant positive correlation with the crown-rump length was observed (Spearman's rho, 0.261; P = 0.006). Neither uterine artery notching nor umbilical artery Doppler parameters correlated with Ang-2 levels. We concluded that Ang-2 as a marker of placental angiogenesis has significant relationships with maternal risk factors associated with abnormal placental development.


Subject(s)
Angiopoietin-2/blood , Placenta/blood supply , Placenta/metabolism , Pregnancy Trimester, First/blood , Adult , Biomarkers/blood , Female , Gestational Age , Humans , Neovascularization, Physiologic , Pregnancy , Risk Factors
14.
Am J Obstet Gynecol ; 201(3): 276.e1-6, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19733278

ABSTRACT

OBJECTIVE: The objective of this study was to determine whether an expanded amniotic fluid cytokine profile predicts spontaneous preterm birth in patients with short cervix in the midtrimester. STUDY DESIGN: Amniocentesis was performed on singleton gestations between 16-24 weeks with a cervical length

Subject(s)
Amniotic Fluid/chemistry , Cervix Uteri/pathology , Pregnancy Outcome , Premature Birth/metabolism , Adult , Cervix Uteri/diagnostic imaging , Chemokine CCL2/analysis , Cytokines , Female , Humans , Pregnancy , ROC Curve , Sensitivity and Specificity , Ultrasonography , Young Adult
15.
J Obstet Gynaecol Res ; 35(4): 672-8, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19751326

ABSTRACT

AIM: Developing a method of maximizing maternal expulsive effort should be of great value in reducing the number of cesarean sections or instrumental deliveries. Various investigations have shown that use of a dental support device (DSD) increases the isometric strength of different muscle groups. The aim of our study was to investigate the role of a DSD in second stage of pushing. METHODS: Nulliparous women with an uncomplicated singleton pregnancy course were randomly assigned either to a DSD group or to a non-device group. Duration of the second stage of labor was evaluated. Rates of cesarean section or instrumental delivery indicated for failure to descend in the second stage of labor were also evaluated. Satisfaction scores for the DSD group were evaluated (range 1-5). RESULTS: Sixty-four subjects were enrolled in the study. Cesarean section and instrumental delivery were performed for 12 (18.8%) and 5 (7.8%) patients, respectively. There was no difference in obstetrical and neonatal demographics between the two groups. Among 64 enrolled patients, 38 (59.3%) were evaluated for the second stage of labor (n = 19 for each group). Duration of the second stage of labor in the DSD group was significantly shorter than in the non-device group: (median 19 min (interquartile interval, IQI, 9) vs 31 min (IQI, 23)), P < 0.001. One patient in the non-device group required a vacuum extraction for failure to descend. The mode of satisfaction score for the DSD group was 5 (59.3%). CONCLUSION: Wearing a dental support device may shorten the second stage of labor, and may decrease the number of failures to descend requiring operative intervention. CLINICAL TRIAL REGISTRATION: NCT00629369.


Subject(s)
Labor Stage, Second , Orthodontic Appliances , Adult , Female , Humans , Infant, Newborn , Muscle Strength , Patient Satisfaction , Pilot Projects , Posture , Pregnancy , Time Factors , Valsalva Maneuver
16.
Am J Obstet Gynecol ; 201(3): 298.e1-6, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19631929

ABSTRACT

OBJECTIVE: Pentraxin (PTX)-3 is an inflammatory molecule that may be increased in the first trimester in pregnancies with subsequent preeclampsia. We measured first-trimester serum PTX-3 and correlated levels with maternal/placental factors related to placental development. STUDY DESIGN: Prospectively enrolled women had ultrasound, physical examination, and blood draw at 11-14 weeks. PTX-3 determined by enzyme-linked immunosorbent assay was related to maternal age, parity, race, body mass index (BMI), mean arterial blood pressure (MAP), smoking/caffeine, and uterine/umbilical artery Doppler pulsatility index (PI). RESULTS: In 111 patients PTX-3 levels ranged from 0.2-13.8 ng/mL. Spearman correlation between PTX-3 and gestational age (rho = 0.096), maternal age (rho = -0.049), BMI (rho = -0.07), MAP (rho = -0.085), mean uterine artery PI (rho = 0.150), and umbilical artery PI (rho = -0.021) was nonsignificant (all P > .05). Similarly, PTX-3 distribution was unaffected by smoking/caffeine use, BMI >30, MAP >100 mm Hg, or uterine artery notching (P > .05 for all). CONCLUSION: First-trimester PTX-3 is unrelated to maternal characteristics and placental Doppler.


Subject(s)
Acute-Phase Proteins/analysis , C-Reactive Protein/analysis , Pregnancy Trimester, First/blood , Serum Amyloid P-Component/analysis , Body Mass Index , Enzyme-Linked Immunosorbent Assay , Female , Gestational Age , Humans , Placenta/diagnostic imaging , Pregnancy , Prospective Studies , Pulsatile Flow/physiology , Ultrasonography, Doppler , Ultrasonography, Prenatal
18.
Endocrine ; 35(1): 81-8, 2009 Feb.
Article in English | MEDLINE | ID: mdl-19016353

ABSTRACT

Vascular smooth muscle cell (VSMC) migration is a pivotal early step in blood vessel remodeling; however, very little is known about the regulation of this process in the human endometrium during the menstrual cycle. In this study, explants of human endometrium were incubated with estradiol and/or progesterone and the conditioned medium (CM) applied to cultures of VSMC to test the hypothesis that estrogen and progesterone stimulate endometrial cells to secrete a factor(s) that promotes VSMC migration. Endometrial explants were composed of highly organized glands and stroma. VSMC migration (cells migrated in 21 h/mm(2) fibronectin-coated semipermeable membrane) in the presence of CM from human endometrial explants obtained in the proliferative phase of the menstrual cycle and incubated for 24 h with estradiol was approximately threefold greater (P < 0.001) than with medium alone and greater (P < 0.05) than with CM from explants treated with estradiol plus progesterone or progesterone. It is concluded, therefore, that estrogen stimulates endometrial secretion of a factor(s) that promotes VSMC migration as an early step in vessel remodeling within the endometrium.


Subject(s)
Cell Movement/drug effects , Cell Movement/genetics , Endometrium/drug effects , Estradiol/pharmacology , Muscle, Smooth, Vascular/drug effects , Angiogenesis Inducing Agents/metabolism , Angiopoietin-1/genetics , Angiopoietin-1/metabolism , Angiopoietin-1/physiology , Cells, Cultured , Culture Media, Conditioned/metabolism , Culture Media, Conditioned/pharmacology , Endometrium/blood supply , Endometrium/metabolism , Female , Follicular Phase/genetics , Follicular Phase/metabolism , Follicular Phase/physiology , Gene Expression/drug effects , Humans , Luteal Phase/genetics , Luteal Phase/metabolism , Luteal Phase/physiology , Microvessels/drug effects , Microvessels/metabolism , Microvessels/physiology , Muscle, Smooth, Vascular/metabolism , Muscle, Smooth, Vascular/physiology , Myocytes, Smooth Muscle/drug effects , Myocytes, Smooth Muscle/metabolism , Myocytes, Smooth Muscle/physiology , Regeneration/drug effects , Regeneration/genetics , Time Factors
19.
J Obstet Gynaecol Res ; 34(1): 100-4, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18226139

ABSTRACT

Although extremely rare, rupture of an unscarred gravid uterus poses significant morbidity and mortality to both fetus and mother. In the past, loss of uterine contraction was thought to be characteristic of uterine rupture, while recent evidence shows that uterine contraction pattern is not associated with uterine rupture. We report two cases of rupture in the unscarred term uterus. Both patients were multiparous and denied any past medical complications, previous cesarean delivery or myomectomy. Uterine rupture occurred in the latent phase of labor at 1 cm dilation (Case 1) and during an intravenous oxytocin infusion after three doses of intravaginal misoprostol (Case 2). Case 2 required cesarean hysterectomy and blood transfusion. Case 1 was monitored with an external tocodynamometer, while Case 2 was monitored with an internal pressure transducer. External monitoring demonstrated the classic sign of complete loss of uterine tone. In contrast, internal monitoring demonstrated an increase in uterine resting tone. Both techniques revealed a stepwise gradual decrease in contraction amplitude followed by sudden onset of profound and prolonged fetal bradycardia (staircase sign). In cases of uterine rupture, differing baseline characteristics between contraction patterns were dependent on uterine monitoring technique. In both techniques a stepwise gradual decrease in contraction amplitude was followed by prolonged fetal bradycardia.


Subject(s)
Fetal Monitoring , Uterine Monitoring , Uterine Rupture/diagnosis , Adult , Bradycardia/embryology , Cesarean Section , Diagnosis, Differential , Female , Fetal Diseases , Humans , Infant, Newborn , Labor Stage, Third , Labor, Induced , Obstetric Labor Complications/diagnosis , Obstetric Labor Complications/physiopathology , Oxytocics/administration & dosage , Pregnancy , Pregnancy Trimester, Third , Uterine Contraction/physiology , Uterine Rupture/physiopathology
20.
Am J Obstet Gynecol ; 198(2): e8-9, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18068141

ABSTRACT

A 35-year-old multipara woman underwent intrauterine pressure catheter placement during labor. Immediately afterwards, she had severe dyspnea develop, became unresponsive, and had a prolonged fetal bradycardia. During emergency cesarean section, she required cardiopulmonary resuscitation repetitively. She then had disseminated intravascular coagulopathy develop and underwent hysterectomy. Anaphylactic reaction may be associated with intrauterine pressure catheter placement.


Subject(s)
Anaphylaxis/diagnosis , Catheterization/adverse effects , Disseminated Intravascular Coagulation/diagnosis , Obstetric Labor Complications/diagnosis , Prenatal Diagnosis , Adult , Anaphylaxis/etiology , Cardiopulmonary Resuscitation , Delivery, Obstetric , Diagnosis, Differential , Disseminated Intravascular Coagulation/etiology , Female , Humans , Infant, Newborn , Obstetric Labor Complications/etiology , Pregnancy , Pressure
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