Comparative Evaluation of the Regenerative Potential of Blood Clot and Platelet-rich Fibrin in Young Permanent Teeth Based on the Revised American Academy of Endodontics Clinical Considerations for Regenerative Procedure: 2016.

Aim: To compare and evaluate the regenerative potential of blood clots and platelet-rich fibrin (PRF) in IYNPT based on the revised American Academy of Endodontics (AAE) clinical considerations for regenerative endodontics 2016. Materials and methods: A total of 20 patients (7-12 years) with immature young necrotic permanent teeth were included and irrigation and disinfection were done using the revised AAE protocol. Teeth were randomly categorized into PRF scaffolding and conventional bleeding technique. The cases were followed up for 1, 3, and 6 months for clinical and radiographic evaluation. Result: At 6 months there was no significant difference between the groups in terms of clinical healing and periapical healing. A significant statistical difference was noted at the end of 6 months with respect to apical closure within the PRF group. A significant difference was seen in the increase in dentin thickness between groups with PRF showing more increase. Conclusion: The PRF scaffold can be used as it induces the regenerative potential of stem cells at the apex. How to cite this article: Prakash AJ, Naik SV, Attiguppe P. Comparative Evaluation of the Regenerative Potential of Blood Clot and Platelet-rich Fibrin in Young Permanent Teeth Based on the Revised American Academy of Endodontics Clinical Considerations for Regenerative Procedure: 2016. Int J Clin Pediatr Dent 2023;16(S-2):S149-S154.

Laser Activation of Aquatine Endodontic Cleanser: A Novel Approach to Root Canal Disinfection.

Aim: To evaluate and compare the antimicrobial efficacy of 2.5% sodium hypochlorite (NaOCl) and Aquatine Endodontic Cleanser (EC) with and without laser-activated disinfection against Enterococcus faecalis in the root canals of primary teeth. Materials and methods: A total of 45 human primary teeth were selected and inoculated with E. faecalis and divided into three groups as per intervention. In group I, irrigation was done using 2.5% NaOCl, in group II using Aquatine EC solution, and in group III with Aquatine EC activated by 810 nm diode laser. Results: Intragroup comparisons showed a decrease in colony-forming units in all three groups. Intergroup comparisons showed a statistically significant difference between group I and group II (p = 0.024) and between group I and group III (p = 0.03). Conclusion: Aquatine EC showed maximum antimicrobial efficacy under laser activation. Clinical significance: Aquatine EC can be considered a suitable alternative to NaOCl in light of its known toxic effects. How to cite this article: Kodical S, Attiguppe P, Siddalingappa RO, et al. Laser Activation of Aquatine Endodontic Cleanser: A Novel Approach to Root Canal Disinfection. Int J Clin Pediatr Dent 2022;15(6):761-763.

CPP-ACP and Fluoride: A Synergism to Combat Caries.

AIMS: The aim of this study is to evaluate and compare the demineralization inhibitory effect, fluoride release at varying time intervals, and antibacterial property of MI varnish and fluor protector varnish. MATERIALS AND METHODS: Twenty-four extracted human premolars were sectioned mesiodistally. The buccal and the lingual halves of the teeth were utilized to evaluate the demineralization inhibitory effect and fluoride release of MI varnish and fluor protector varnish, respectively. To evaluate the demineralization inhibitory effect, 150-µm axial longitudinal section was obtained that was photographed and evaluated under a polarized light microscope. To evaluate the fluoride release, a fluoride-specific ion electrode was used. To evaluate the antibacterial susceptibility, the disk diffusion test (Kirby-Bauer method) was done. RESULTS: Statistical analysis was done using the Students unpaired t test for intergroup comparison and the p value <0.01 was obtained for all the three parameters, i.e., demineralization inhibitory effect, antimicrobial property, and fluoride releasing property at different time intervals. CONCLUSION: A combination of casein phosphopeptide (CPP)-amorphous calcium phosphate (ACP) in fluoride varnish seems to enhance the caries preventive potential of fluoride varnish and antibacterial activity against Streptococcus mutans. CLINICAL SIGNIFICANCE: MI varnish, a combination of CPP-ACP and fluoride varnish could be the future of minimally invasive dentistry as it is minimally invasive yet an effective modality for caries prevention. HOW TO CITE THIS ARTICLE: Attiguppe P, Malik N, et al. CPP-ACP and Fluoride: A Synergism to Combat Caries. Int J Clin Pediatr Dent 2019;12(2):120-125.

Nanotechnology in Dentin Disinfection: Can We Preserve the Bond?

AIMS: The aim of this study is to evaluate the effect of cavity disinfection with 2% chlorhexidine (CHX) and Ag-Au nanoparticles on microleakage and resin tag penetrability of composite restoration under in vitro conditions. MATERIALS AND METHODS: Twenty-five human permanent molars extracted for therapeutic reasons were used in the study. Class V cavity of standard dimension was prepared on the buccal and lingual surfaces of the teeth. The teeth were randomly allocated into two groups based on the cavity disinfectant used: group I being 2% CHX gluconate (chlorhexidina Friedrich and Bianca Mittelstadt (FGM)) and group II being cavity disinfectant containing Ag-Au nanoparticles (nanocare gold). In both the groups, the dentin was etched with 37% phosphoric acid and cavity disinfectants were applied following which dentine bonding agent and composite resin were applied and cured. The specimens were then viewed under a stereomicroscope and a scanning electron microscope for microleakage and resin tag formation, respectively. RESULTS: The results were statistically analyzed using an independent "t" test. No significant difference was seen between the two groups with respect to both microleakage and resin tag penetration values (p > 0.05). CONCLUSION: The cavity disinfectant containing Ag-Au nanoparticles did not affect the sealing ability and resin tag penetrability of composite resin in permanent molars when compared with 2% CHX. HOW TO CITE THIS ARTICLE: Attiguppe P, Tripathi AP, et al. Nanotechnology in Dentin Disinfection: Can We Preserve the Bond? Int J Clin Pediatr Dent 2019;12(1):42-46.

Comparative Evaluation of Different Modes of Laser Assisted Endodontics in Primary Teeth: An In vitro Study.

INTRODUCTION: The presence of bacteria in root canals has been considered to be responsible for endodontic treatment failure, even in case of primary teeth. The use of lasers can be a valuable addition in removing bacterial load in areas where traditional methods may fail to succeed. Methods like direct laser irradiation of canals, Laser Activated Irrigation (LAI) with sodium hypochlorite (NaOCl) and Photodynamic Therapy (PDT) seem to be a promising alternative for disinfection. AIM: This study was designed to compare the efficacy of latest advancements in disinfection techniques using diode laser namely direct laser irradiation, photodynamic therapy and laser activated irrigation using sodium hypochlorite. MATERIALS AND METHODS: Sixty freshly extracted primary teeth either single or multi-rooted teeth with two third of their root length intact were collected. Instrumentation was completed to size 30 H-file. Teeth were randomly divided into Group 1- Direct Laser- irradiation, Group 2 - Photodynamic therapy; Group 3- Laser activated irrigation with 2.5% NaOCl. The tooth specimens were inoculated with Enterococcus faecalis. The bacterial colonies were counted preoperatively. Laser irradiation was performed for all groups in accordance to the groups each tooth belonged to. Postoperatively the bacterial colonies were counted. One-way Analysis was applied to compare bacterial count at baseline and post-test between three groups. Tukey's post-hoc test was applied for pairwise comparison between groups. Paired t-test was applied to compare the mean baseline bacterial count with post-test mean bacterial count. RESULTS: The results obtained with all the three groups postoperatively were highly significant (p-value<0.001). Statistically significant difference between results of Group 1 and Group 2 and also between Group 1 and Group 3 was found (p-value≤ 0.001). However, no statistical difference between Group 2 and Group 3 was found (p-value- 0.96). CONCLUSION: Disinfection strategies using diode laser by techniques gives promising results. Techniques like laser activated irrigation and photoactivated disinfection are better than direct laser irradiation in eliminating Enterococcus feacalis.

Presurgical nasoalveolar molding: A boon to facilitate the surgical repair in infants with cleft lip and palate.

Cleft lip and palate (CLP) is the most common congenital craniofacial anomaly. Rehabilitation of CLP generally requires a team approach. Alveolar and nasal reconstruction for these patients is a challenge for the reconstructive surgeon. Various procedures have been attempted to reduce the cleft gap, so as to obtain esthetic results postsurgically. The presurgical nasoalveolar molding (PNAM) technique, developed by Grayson, is a new approach to presurgical infant orthopedics. PNAM reduces the severity of the initial cleft alveolar and nasal deformity. Thus, it enables the surgeon and the patient to enjoy the benefits associated with repair of a cleft deformity that is minimal in severity. This article presents a brief insight into PNAM with a case series of three different cases (one unilateral and two bilateral) which underwent PNAM treatment and gave an excellent surgical prognosis.

Age Assessment in Children: A Novel Cameriere's Stratagem.

AIM: Age is one of the essential factors in establishing the identity of a person, especially in children. Age estimation plays an important part in treatment planning, forensic dentistry, legal issues, and paleodemographic research. The present study was an attempt to estimate the chronological age in children of Davangere population by using Cameriere's India specific formula. MATERIALS AND METHODS: This was a retrospective observational study to estimate the chronological age in children of Davangere population. A total of 150 panoramic radiographs of patients aged between 6 and 15 years, including both sexes, were selected. Age was calculated by measuring open apices of seven right or left mandibular teeth using Adobe Photoshop software. RESULTS: Statistical analysis was performed to derive a regression equation for estimation of age, which showed that, of the variables X1, X2, X3, X4, X5, X6, X7, s, N0, the variables N0 and X4 were statistically noteworthy. Hence, these two variables were used to derive the linear regression formula: Age = 10.522 + 0.712(N0) - 5.040(X4). The model was found to be statistically significant, F(2, 147) = 207.96, p < 0.001, and accounted for approximately 74% of the variance of age (R2 = 0.739, adjusted R2 = 0.735). CONCLUSION: Cameriere's method can be used for age assessment in children for forensic as well as legal contexts and based on these variables a reliable age estimation equation could be proposed specifically for Davangere population. HOW TO CITE THIS ARTICLE: Attiguppe PR, Yavagal C, Maganti R, Mythri P. Age Assessment in Children: A Novel Cameriere's Stratagem. Int J Clin Pediatr Dent 2016;9(4):330-334.