1.
Biochem Soc Trans
; 25(1): 98S, 1997 Feb.
Article
in English
| MEDLINE
| ID: mdl-9056996
2.
Curr Genet
; 29(5): 462-7, 1996 Apr.
Article
in English
| MEDLINE
| ID: mdl-8625426
ABSTRACT
Strains of Aspergillus nidulans carrying the sltA1 mutation, conferring sensitivity to KCl and NaCl, also showed an arginine-sensitive phenotype whereby concentrations of the L-amino acid at or above 10 mM were toxic to growth. Sexual progeny of a cross between a sltA1 mutant and a wild-type strain showed a co-segregation of salt and arginine sensitivity. Similarly, revertants to salt tolerance showed a loss of arginine sensitivity as did sltA1 strains that were transformed with a cosmid carrying the putative sltA1+ wild-type allele. In addition, arginine sensitivity could be relieved by L-ornithine. It is suggested that sltA1 is a salt-sensitive allele of the arginase gene (agaA).
Subject(s)
Alleles , Arginase/genetics , Aspergillus nidulans/genetics , Chlorides/pharmacology , Genes, Fungal , Arginine/analogs & derivatives , Arginine/pharmacology , Aspergillus nidulans/drug effects , Aspergillus nidulans/enzymology , Aspergillus nidulans/growth & development , Crosses, Genetic , Drug Resistance, Microbial , Meiosis , Mutagenesis , Phenotype , Potassium Chloride/pharmacology , Protoplasts/drug effects , Sodium Chloride/pharmacology , Transformation, Genetic
3.
Biochem Soc Trans
; 23(1): 24S, 1995 Feb.
Article
in English
| MEDLINE
| ID: mdl-7538946