[The cooperative effect of hydrocortisone, adrenaline, and high density lipoproteins in regulating the activity of multiple forms of liver hexokinase]. / O kooperativnom éffekte gidrokortizona, adrenalina i lipoproteidov vysokoi plotnosti v reguliatsii aktivnosti mnozhestvennykh form geksokinazy v pecheni.

High density lipoproteins (HDL) were shown to modulate the effect of hydrocortisone and adrenaline on activity of hexokinase in surviving liver tissue slices. The hormones inhibited the enzymatic activity while in presence of HDL the hormones exhibited an opposite effect. The phenomenon observed was termed a "cooperative effect"; it could not be reproduced by any single components of the system studied. Mechanism of the effect involved induction of all the multiple hexokinase forms which was abolished by actinomycin D. A specific function could be ascribed only to HDL3; HDL2, low and very low density lipoproteins did not exhibit any effects. The cooperative effect of HDL and of the adaptive hormones was also inhibited in the surviving liver tissue slices by vinblastine, gordox and methylamine thus demonstrating the sophisticated nature of the mechanism.

[The cooperative effect of hydrocortisone, adrenaline and high-density lipoproteins in regulating liver hexokinase activity]. / Kooperativnyi éffekt gidrokortizona, adrenalina i lipoproteidov vysokoi plotnosti v reguliatsii aktivnosti geksokinazy pecheni.

The authors described a cooperative effect of glucocorticoids, catecholamines and high density lipoproteins (HDLP), based on increased hexokinase and glucokinase biosynthesis in the liver, resulting in elevated activity of the respective enzymes. This effect was completely blocked by actinomycin-D (a DNA-dependent inhibitor of RNA synthesis), vinblastine (an inhibitor of the microtubular apparatus and intracellular shift) and gordox (an inhibitor of serine proteinases). The modulating effect of HDLP with relation to the adaptive hormones emphasizes the cooperative nature of the new mechanism of enzymatic synthesis induction.

[Effect of prodigiozan on the glucocorticoid and insulin ratio in the blood serum of white rats]. / Vliianie prodigiozana na sootnoshenie gliukokortikoidov i insulina v syvorotke krovi belykh krys.

Significant hypertrophy of the adrenal glands was observed in Wistar rats the day after a single prodigiozan administration. This did not change the level of glucocorticoids in blood serum, but increased the concentration in insulin. Analysis of steroidogenesis in the adrenal glands indicated significant increase in steroid's production in the experimental group of rats as compared to the control. An increase of glucoso-6-phosphate dehydrogenase and hexokinase activity in adrenal tissue was also observed. The results suggest that stimulation of corticosteroid's secretion was accompanied by an increase in their reception to tissues which caused an increase of nonspecific resistance of the organism.