ABSTRACT
Induced Pluripotent Stem Cell (iPSC) lines derived from healthy individuals are helpful and essential tools for disease modelling. Here, we described the reprogramming of skin fibroblasts obtained from a healthy 59-year-old individual without Alzheimer's disease. The generated iPSC lines have a normal karyotype, expressed pluripotency markers, and demonstrated the ability to differentiate into the three germ layers. The iPSC lines will be used as controls to study Alzheimer's disease mechanisms.
Subject(s)
Alzheimer Disease , Induced Pluripotent Stem Cells , Humans , Middle Aged , Induced Pluripotent Stem Cells/metabolism , Alzheimer Disease/metabolism , Fibroblasts , Germ Layers , Cell Differentiation , Cellular ReprogrammingABSTRACT
Alzheimer's disease (AD) is a progressive neurological disorder and the most common form of dementia worldwide. Sporadic Alzheimer's disease (sAD) cases are the main forms, over 95% of AD cases, but still poorly understood. Thereby there is a crucial need to develop in vitro models for studying this multifactorial disorder. Here, we report the reprogramming of skin fibroblasts from a 57-years-old male donor. The new generated iPSC cell line has a normal karyotype and, is pluripotent since it demonstrates the ability to differentiate in vitro into the three germ layers. This iPSC line will be used to understand pathological mechanisms of sAD.
Subject(s)
Alzheimer Disease , Induced Pluripotent Stem Cells , Humans , Male , Middle Aged , Induced Pluripotent Stem Cells/metabolism , Alzheimer Disease/pathology , Cell Line , Fibroblasts/metabolism , Germ Layers/metabolism , Cell DifferentiationABSTRACT
Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 58â¯year-old woman suffering from Alzheimer's disease and carrying a D694N mutation on Amyloid precursor protein (APP). Fibroblasts were reprogrammed into iPSC using the integration-free Sendai Virus which allows the expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. The cells have the corresponding mutation and present a normal karyotype. The reported APP-D694N iPSC line may be used to model and study human AD pathology in vitro.
Subject(s)
Alzheimer Disease/genetics , Amyloid beta-Protein Precursor/genetics , Cell Differentiation , Cellular Reprogramming , Fibroblasts/pathology , Induced Pluripotent Stem Cells/pathology , Mutation , Alzheimer Disease/pathology , Cells, Cultured , Female , Fibroblasts/metabolism , Heterozygote , Humans , Induced Pluripotent Stem Cells/metabolism , Middle Aged , PhenotypeABSTRACT
Induced pluripotent stem cells (iPSC) were generated from skin fibroblasts obtained from a 50â¯year-old patient suffering from Alzheimer's disease and carrying a G217D causal mutation on presenilin 1 (PSEN1). iPSCs were obtained following reprogramming using the integration-free Sendai Virus system which allows expression of the Yamanaka factors. Verification of their pluripotency was achieved by demonstrating the expression of pluripotency markers and their differentiation potential into the three primary germ layers. iPS cells carry the patient G217D mutation and present a normal karyotype. The reported PS1-G217D iPSC line may be used to model and study human AD pathology in vitro.
