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1.
Diabetes Care ; 35(5): 945-7, 2012 May.
Article in English | MEDLINE | ID: mdl-22338101

ABSTRACT

OBJECTIVE: To determine whether a mindfulness-based stress reduction (MBSR) intervention is effective for reducing psychosocial distress (i.e., depression, psychosocial stress) and the progression of nephropathy (i.e., albuminuria) and for improving the subjective health status of patients with type 2 diabetes. RESEARCH DESIGN AND METHODS: Patients with type 2 diabetes and microalbuminuria were randomized to a mindfulness-based intervention (n = 53) or a treatment-as-usual control (n = 57) group. The study is designed to investigate long-term outcomes over a period of 5 years. We present data up to the first year of follow-up (FU). RESULTS: At FU, the MBSR group showed lower levels of depression (d = 0.71) and improved health status (d = 0.54) compared with the control group. No significant differences in albuminuria were found. Per-protocol analysis also showed higher stress reduction in the intervention group (d = 0.64). CONCLUSIONS: MBSR intervention achieved a prolonged reduction in psychosocial distress. The effects on albuminuria will be followed up further.


Subject(s)
Diabetes Mellitus, Type 2/psychology , Diabetes Mellitus, Type 2/therapy , Stress, Psychological/prevention & control , Adult , Aged , Depression/prevention & control , Female , Humans , Male , Meditation/methods , Middle Aged , Treatment Outcome
2.
Brain ; 132(Pt 12): 3342-52, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19952055

ABSTRACT

We established microRNA profiles from active and inactive multiple sclerosis lesions. Using laser capture microdissection from multiple sclerosis lesions to pool single cells and in vitro cultures, we assigned differentially expressed microRNA to specific cell types. Astrocytes contained all 10 microRNA that were most strongly upregulated in active multiple sclerosis lesions, including microRNA-155, which is known to modulate immune responses in different ways but so far had not been assigned to central nervous system resident cells. MicroRNA-155 was expressed in human astrocytes in situ, and further induced with cytokines in human astrocytes in vitro. This was confirmed with astrocyte cultures from microRNA-155-|-lacZ mice. We matched microRNA upregulated in phagocytically active multiple sclerosis lesions with downregulated protein coding transcripts. This converged on CD47, which functions as a 'don't eat me' signal inhibiting macrophage activity. Three microRNA upregulated in active multiple sclerosis lesions (microRNA-34a, microRNA-155 and microRNA-326) targeted the 3'-untranslated region of CD47 in reporter assays, with microRNA-155 even at two distinct sites. Our findings suggest that microRNA dysregulated in multiple sclerosis lesions reduce CD47 in brain resident cells, releasing macrophages from inhibitory control, thereby promoting phagocytosis of myelin. This mechanism may have broad implications for microRNA-regulated macrophage activation in inflammatory diseases.


Subject(s)
Brain/metabolism , CD47 Antigen/genetics , CD47 Antigen/metabolism , MicroRNAs/genetics , Multiple Sclerosis/genetics , Multiple Sclerosis/metabolism , Adult , Animals , Astrocytes/metabolism , Autoimmunity/genetics , Autoimmunity/immunology , Brain/pathology , Brain/physiopathology , Cells, Cultured , Female , Gene Expression Profiling/methods , Humans , Macrophages/immunology , Macrophages/metabolism , Male , Mice , Mice, Knockout , MicroRNAs/analysis , Microdissection , Middle Aged , Multiple Sclerosis/physiopathology , Myelin Sheath/immunology , Myelin Sheath/metabolism , Phagocytosis/genetics , Phagocytosis/immunology , Polymerase Chain Reaction
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