Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Biosci Biotechnol Biochem ; 82(2): 285-291, 2018 Feb.
Article in English | MEDLINE | ID: mdl-29338640

ABSTRACT

Cowpea seed ß-vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of ß-vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins.


Subject(s)
Globulins/chemistry , Globulins/metabolism , Plant Proteins/chemistry , Plant Proteins/metabolism , Vigna/chemistry , Glycosylation , Metals/metabolism , Seeds/chemistry
SELECTION OF CITATIONS
SEARCH DETAIL
...