Selenium dietary intake and survival among CRC patients.

Background: Despite advances in prevention and treatment, colorectal cancer remains the second most common cause of cancer death. To date, little is known about the role of prediagnostic selenium intake in colorectal cancer survival. Objective: The purpose of the study was to verify whether selenium intake in habitual diet before diagnosis is associated with survival in colorectal cancer patients. Study design: This was a prospective observation of patients primarily recruited for a case-control study between 2000 and 2012 in Cracow, Poland. A group of 671 incident cases of colorectal cancer was included. Habitual diet was assessed using a validated 148-item food questionnaire. 338 deaths were identified throughout 2017 by the Polish National Vital Registry. To evaluate the impact of dietary selenium on survival, the multivariable Cox regression model was used. Results: After standardization for several potential confounders (including key determinants, such as radical surgery, chemotherapy, tumor stage, and dietary factors), a decrease in the risk of death from colorectal cancer was observed in the group with higher dietary selenium intake (≥48.8 µg/day, group mean: 63.9 µg/day) compared to the group with lower dietary selenium intake (<48.8 µg/day, mean: 38.5 µg/day) (HR=0.73; 95% CI: 0.54-0.98) (the median was used for categorization). Conclusion: Our study suggests selenium as an additional dietary factor which may be associated with survival among colorectal cancer patients referred to surgery. Due to the observational nature of the study, the results should be taken with caution. These preliminary findings, however, provide the basis for well-structured clinical trials.

Calcium intake may explain the reduction of colorectal cancer odds by dietary selenium - a case-control study in Poland.

BACKGROUND: Colorectal cancer (CRC) has been placed among top three cancer sites in high income countries. Although there are several inconsistencies across studies it is widely accepted that diet contributes to approximately 70% of CRC. Several dietary factors have been investigated; however, the knowledge about the role of trace elements and their interplay with other dietary factors in CRC odds is limited. The aim of the study was to estimate the odds ratio of colorectal cancer associated with the content of selenium in diet, and to check whether dietary calcium is a modifier of selenium effect in the population characterized by low selenium intake. METHODS: Face-to-face interviews were used to gather data on dietary habits (by 148-item semi-quantitative food frequency questionnaire) and covariates among 683 histologically confirmed incident colorectal cancer cases and 759 hospital-based controls in a case-control study. Data was collected in a period between 2000 and 2012. SETTING: Lesser Poland, Central Europe. Logistic regression models were used to assess the role of dietary selenium intake and calcium-selenium interaction in colorectal cancer odds. RESULTS: After the adjustment for several covariates dietary selenium was associated with the decrease of colorectal cancer odds by 8% (OR = 0.92, 95%CI: 0.84-0.99 for every 10µg Se/day increase). In individuals with lower (< 1000 mg/day) calcium content the odds of colorectal cancer was decreased by 13%(for every 10µg Se/day) and by 44% and 66% depending on the categories of selenium intake (60 to < 80 µg/day and ≥ 80 µg/day, respectively). The effect of dietary selenium was modified by dietary calcium (p for interaction < .005). CONCLUSIONS: The study has shown a beneficial effect of dietary selenium for colorectal cancer and a modification effect of dietary calcium in a population characterized by lower levels of selenium intake. The results provide the basis for well-planned controlled trials to confirm the findings.

Whole-cell pertussis vaccine (DTwP) has no influence on allergic diseases and atopic sensitization in children.

INTRODUCTION: Vaccine opponents indicate that the infant's immune system is inadequately developed to handle multiple vaccines which may overwhelm the immune system, leading to allergic diseases. AIM: To verify the association between the vaccine antigen overload derived from DTwP and the development of atopic sensitization and allergic diseases. MATERIAL AND METHODS: Data from an earlier established birth cohort in Krakow, followed up to the 6th year of life were used. Allergic diseases such as eczema, hay fever and asthma were diagnosed by a physician and reported every half a year from the 1st to 6th year of life by the child's parent. Skin prick tests (SPT) were performed in children at 5 years of age. The data on infants' vaccination were extracted from the physician's records. The status of vaccine antigen exposure was based on different types of vaccines against pertussis (DTwP or DTaP) in a primary course. Results were determined by multiple logistic regression, adjusted to potential confounders. RESULTS: The analyzed population consisted of 234 children: 53.4% - boys and 46.6% - girls. Infants up to the age of 8 months were vaccinated with the primary course against pertussis, with DTwP - 60.7%, DTaP - 32.9% and further 6.4% with a mixed course (DTwP + DTaP). There were no significant relationships between any of vaccination groups and allergic disease and allergen sensitivity in the multiple logistic regression model with adjustment to potential confounders. CONCLUSIONS: The exposure to a large number of vaccine antigens derived from DTwP has no influence on the development of allergic diseases and atopic sensitization in children.

Experience with Saccharomyces boulardii Probiotic in Oncohaematological Patients.

Very few reports have been published to date on the bloodstream infections caused by Saccharomyces spp. in oncohaematological patients, and there are no guidelines on the use of this probiotic microorganism in this population. We describe the use of probiotic preparation containing Saccharomyces boulardii in a large group of oncohaematological patients. We retrospectively analysed the data from 32,000 patient hospitalisations at the haematological centre during 2011-2013 (including 196 haematopoietic stem cell transplant recipients) in a tertiary care university-affiliated hospital. During the study period, 2270 doses of Saccharomyces boulardii probiotic were administered to the oncohaematological patients. In total, 2816 mycological cultures were performed, out of which 772 (27.4%) were positive, with 52 indicating digestive tract colonisation by Saccharomyces spp., mainly in patients with acute myeloid leukaemia (AML), myelodysplastic syndrome (MDS) or multiple myeloma (MM). While colonised, they were hospitalised for 1683 days and 416 microbiological cultures of their clinical samples were performed. In the studied group of patients, there were six blood cultures positive for fungi; however, they comprised Candida species: two C. glabrata, one C. albicans, one C. krusei, one C. tropicalis and one C. parapsilosis. There was no blood culture positive for Saccharomyces spp. Our study indicates that despite colonisation of many oncohaematological patients with Saccharomyces spp., there were no cases of fungal sepsis caused by this species.

The effectiveness of rotavirus vaccine in preventing acute gastroenteritis during rotavirus seasons among Polish children.

INTRODUCTION: Rotavirus is the main etiological cause of intestinal infections in children. Voluntary rotavirus vaccines were included in the Polish vaccination schedule in 2007. The aim of this study was to assess the effectiveness of a completed rotavirus vaccination course in preventing acute gastroenteritis in Polish infants during their first five years of life. MATERIAL AND METHODS: This was a retrospective cohort study conducted in Lesser Poland (Malopolska Province). The sample population included a group of 303 children who received the completed rotavirus vaccination course and 303 children not vaccinated against rotavirus. The date of the child's acute gastroenteritis diagnosis and his or her vaccination history were extracted from the physicians' records. Each kind of diagnosed acute gastroenteritis during winter-spring rotavirus seasons was treated as the endpoint. The relative risk of having gastrointestinal infection was assessed using the hazard ratio from the Cox proportional hazards regression model. RESULTS: In the examined group, 96 (15.8%) children had winter-spring gastrointestinal infections. In the non-vaccinated children, the cumulative incidence of these infections in the first 5 years of life was 20.8%, whereas in the children vaccinated with Rotarix it was only 10.9%. Those who were vaccinated with Rotarix had a 44% reduction in the risk of a winter-spring acute gastroenteritis infection compared to those not vaccinated with Rotarix (p = 0.005). Birth weight less than 2500 g increased the risk of the infection twofold and also reached statistical significance (p = 0.044). CONCLUSIONS: The results showed that Rotarix is effective in preventing acute gastroenteritis in Polish children during rotavirus seasons.

Sensitivity of passive diarrhea surveillance in infants up to the second year of life in Malopolskie voivodeship.

INTRODUCTION: In EU countries and also in Poland, the surveillance of infectious acute gastro-enteritis is a mandatory system based on cases notification (suspected and confirm) conducted by physicians in hospitals and ambulatory care. STUDY OBJECTIVE: The assessment of surveillance sensitivity on acute diarrhea in children up to 2nd year of life in Malopolskie voivodeship from 2009 to 2012. MATERIAL AND METHODS: The data about 720 infants surveyed respectively from birth up to 2nd year of life were used in this study. The information on acute diarrhea cases (outpatient and hospitalized) occurred in infants during follow-up was included in analysis. The data derived from medical records. Each case of acute diarrhea in infants detected by medical records was surveyed in local surveillance system on infectious diseases whether it was notified by physician. RESULTS: The surveillance sensitivity on acute diarrhea in infants up to 2nd year of life increased in Malopolskie voivodeship from 14.3% in 2009 to 24.3% in 2012. The sensitivity of surveillance on hospitalized cases was twofold higher compared to all kind of cases assessed inclusively. CONCLUSION: The surveillance sensitivity on acute diarrhea in infants up to 2nd year of life improved significantly in recent years but is still too low, especially with respect to outpatient cases.

Stability of the mental and motor Bayley Scales of Infant Development (2nd ed.) in infants over first three years of life.

UNLABELLED: Despite the Bayley Scales of Infant Development second edition (BSID-II) are wide used both clinically and in research settings, only a few published studies have been examined their stability over time. AIM OF STUDY: The aim of this study was to examine the stability of mental and motor BSID-II scores over the first three years of life. MATERIAL AND METHODS: All children included in this study were a sample followed up in a study on the susceptibility of the fetus and child to environmental factors. The cohort recruited prenatally in Krakow Poland, included 408 children. The mental and motor scales of BSID-II were administered to infants at the end of 12th, 24th and 36th month of life. Stability of the test scores from first to second and third assessment was evaluated using the Pearson's correlation coefficient calculated for the entire group of infants, and for the each gender separately. RESULTS: The older infants obtained the better outcomes in BSID-II. The correlation between the first and second assessments for the Psychomotor Developmental Index (PDI) was r = 0.30, for the Mental Developmental Index (MDI) was r = 0.33, and between the second and third assessments the correlation for the PDI was r = 0.40, for the MDI was r = 0.59. That results suggest a low or moderate degree of relationship between the tests outcomes. Only 8,8% to 34,8% of variance in the infants later BSID-II scores could be explained by their earlier scores. The correlation between the tests scores was higher between outcomes obtained by girls than boys. CONCLUSION: The BSID-II should not be treated as a useful measure for predictive purposes of infants development.

Usefulness of maternal assessment of children development based on reported age of achieved milestones.

STUDY OBJECTIVE: The aim of the study was to examine the usefulness of maternal recall of selected developmental milestones by testing their correlations with the Bayley Scales of Infant Development (BSID-II). MATERIAL AND METHODS: Prospective cohort study. The cohort recruited prenatally, included 387 children. The BSID-II tests were carried out in each child at the end of the 12th, 24th and 36th month of life. When children were 3 years old, mothers were questioned about their child's age at attainment of 8 significant developmental milestones. RESULTS: The infants who attained developmental milestones earlier in their first years of life were more likely to achieved a better scores on the motor scale of the BSID-II. Correlation coefficients ranged from -0.117 for bladder control to -0.424 for standing without assistance and -0.586 for walking unassisted. Correlation arose when the difference between the time of achieving a particular milestone and time of managing the BSID-II was smaller. CONCLUSION: Our study demonstrated that maternal reports of developmental milestones of children under 3 years old are sufficiently reliable to be used in clinical judgment.

Does dietary calcium interact with dietary fiber against colorectal cancer? A case-control study in Central Europe.

BACKGROUND: An unfavorable trend of increasing rates of colorectal cancer has been observed across modern societies. In general, dietary factors are understood to be responsible for up to 70% of the disease's incidence, though there are still many inconsistencies regarding the impact of specific dietary items. Among the dietary minerals, calcium intake may play a crucial role in the prevention. The purpose of this study was to assess the effect of intake of higher levels of dietary calcium on the risk of developing of colorectal cancer, and to evaluate dose dependent effect and to investigate possible effect modification. METHODS: A hospital based case-control study of 1556 patients (703 histologically confirmed colon and rectal incident cases and 853 hospital-based controls) was performed between 2000-2012 in Krakow, Poland. The 148-item semi-quantitative Food Frequency Questionnaire to assess dietary habits and level of nutrients intake was used. Data regarding possible covariates was also collected. RESULTS: After adjustment for age, gender, education, consumption of fruits, raw and cooked vegetables, fish, and alcohol, as well as for intake of fiber, vitamin C, dietary iron, lifetime recreational physical activity, BMI, smoking status, and taking mineral supplements, an increase in the consumption of calcium was associated with the decrease of colon cancer risk (OR = 0.93, 95% CI: 0.89-0.98 for every 100 mg Ca/day increase). Subjects consumed >1000 mg/day showed 46% decrease of colon cancer risk (OR = 0.54, 95% CI: 0.35-0.83). The effect of dietary calcium was modified by dietary fiber (p for interaction =0.015). Finally, consistent decrease of colon cancer risk was observed across increasing levels of dietary calcium and fiber intake. These relationships were not proved for rectal cancer. CONCLUSIONS: The study confirmed the effect of high doses of dietary calcium against the risk of colon cancer development. This relationship was observed across different levels of dietary fiber, and the beneficial effect of dietary calcium depended on the level of dietary fiber suggesting modification effect of calcium and fiber. Further efforts are needed to confirm this association, and also across higher levels of dietary fiber intake.

Measles, mumps and rubella (MMR) vaccination has no effect on cognitive development in children - the results of the Polish prospective cohort study.

OBJECTIVES: The aim of the study was to examine the hypothesis that MMR exposure has a negative influence on cognitive development in children. Furthermore, MMR was compared to single measles vaccine to determine the potential difference of these vaccines safety regarding children's cognitive development. METHODS: The prospective birth cohort study with sample consisted of 369 infants born in Krakow. Vaccination history against measles (date and the type of the vaccine) was extracted from physicians' records. Child development was assessed using the Bayley Scales of Infant Development (BSID-II) up to 3rd year of life, Raven test in 5th and 8th year and Wechsler (WISC-R) in 6th and 7th year. Data on possible confounders came from mothers' interview, medical records and analyses of lead and mercury level at birth and at the end of 5th year of life. Linear and logistic regression models adjusted for potential confounders were used to assess the association. RESULTS: No significant differences in cognitive and intelligence tests results were observed between children vaccinated with MMR and those not vaccinated up to the end of the 2nd year of life. Children vaccinated with MMR had significantly higher Mental BSID-II Index (MDI) in the 36th month than those vaccinated with single measles vaccine (103.8±10.3 vs. 97.2±11.2, p=0.004). Neither results of Raven test nor WISC-R were significantly different between groups of children vaccinated with MMR and with single measles vaccine. After standardization to child's gender, maternal education, family economical status, maternal IQ, birth order and passive smoking all developmental tests were statistically insignificant. CONCLUSION: The results suggest that there is no relationship between MMR exposure and children's cognitive development. Furthermore, the safety of triple MMR is the same as the single measles vaccine with respect to cognitive development.

The frequency and risk factors of allergy and asthma in children with autism--case-control study.

INTRODUCTION: The evolution of autistic disorders in children depends on many factors, like concomitance of the other diseases, which can escalate the autistic symptoms. One of those groups are allergic diseases, which have one of the highest prevalence rates in children. OBJECTIVES: The aim of this analysis was to determine the frequency of asthma and allergy in children with autism in comparison to controls and the risk factors of allergic diseases and asthma in both groups. MATERIAL AND METHODS: Study population included 96 cases diagnosed with childhood or atypical autism and 192 controls matched individually by year of birth, gender and physician's practice. The analysis was performed in each group separately giving possibility to compare the results between study groups. RESULTS: The frequency of asthma and allergic diseases in both groups has not revealed any statistically significant differences. Children with autism have been affected by asthma in 5,2% and by allergy in 25,0%, controls in 4,7% and 21,9% respectively. All cases of asthma was diagnosed in boys, commonly allergy was also more frequent in boys than girls in both studied groups. However those differences was statistically insignificant. The father's allergy and asthma was revealed as a risk factor of allergy in children with autism. In controls additionally allergy or asthma diagnosed in mother or grandparent increased risk of allergy in children. CONCLUSIONS: Children with autism were affected by asthma and allergy with similar frequency like children without autistic disorders. Allergy in father was the risk factor of allergic diseases in children with autism.

[The influence of rotavirus vaccinations on the risk of gastrointestinal infections in children. Methods and preliminary results]. / Wplyw szczepien przeciwko rotawirusom na wystepowanie infekcji jelitowych u dzieci--metodyka oraz wyniki badania pilotazowego.

UNLABELLED: The gastrointestinal infection in infants are the significant health and epidemiological issue. The voluntary rotavirus vaccines was included to Polish vaccination schedule in 2007. AIM OF THE STUDY: Aim of the study was the assessment of rotaviruses vaccines effectiveness in prevention of rotavirus gastrointestinal infections requiring hospitalization and the influence or these vaccinations on the risk of gastrointestinal outpatient infections. The additional aim of the study was determine the incidence of adverse events following immunization caused by rotavirus vaccinations and comparison of both vaccines--Rotarix and RotaTeq with respect to the mentioned objectives. MATERIALS AND METHODS: The retrospective cohort study was designed to achieve the aims of study. The study cohort included infants from Malopolska Voivodeship vaccinated with rotavirus vaccine in 2007-2008 and eligible matched unvaccinated children. The preliminary study was conducted in 2011. RESULTS: The cohort study included 74 vaccinated and 74 unvaccinated infants. The preliminary analysis revealed that infants vaccinated against rotavirus had 40% lower risk to be affected by gastrointestinal infections in comparison to unvaccinated children. Nevertheless the results were not statistically significant owing to the scarce number of infants in pilot study group (95% CI: 0,27-1,30). CONCLUSION: The preliminary results indicates, that the rotavirus vaccines have the positive influence on the decrease risk of gastrointestinal infections in children up to 2 years of years but the evidence is not sufficient yet, to be conclusive. The ongoing survey is given the possibility to gain the bigger cohort under study and to attain the presumed objectives.

Neonatal exposure to Thimerosal from vaccines and child development in the first 3 years of life.

BACKGROUND: Despite the common use of Thimerosal as a preservative in childhood vaccines since the 1930s, there are not many studies on ethylmercury toxicokinetics and toxicodynamics in infants. The knowledge of ethylmercury's potential adverse effects is derived mostly from parallel methylmercury research or from animal and theoretical models. AIM OF THE STUDY: This study was designed to examine the relationship between neonatal exposure to Thimerosal-containing vaccine (TCV) and child development. MATERIAL AND METHODS: The study sample consisted of 196 infants born between January 2001 and March 2003 to mothers attending ambulatory prenatal clinics in the first and second trimesters of pregnancy in Krakow. Vaccination history (date and the type of the vaccine) was extracted from physicians' records. Child development was assessed using the Bayley Scales of Infant Development (BSID-II) measured in one-year intervals over 3years. General Linear Model (GLM) and Generalized Estimating Equation (GEE) models adjusted for potential confounders were used to assess the association. RESULTS: An adverse effect of neonatal TCV exposure was observed for the psychomotor development index (PDI) only in the 12th and 24th months of life (ß=-6.44, p<0.001 and ß=-5.89, p<0.001). No significant effect of neonatal TCV exposure was found in the 36th month. The overall deficit in the PDI attributable to neonatal TCV exposure measured over the course of the three-year follow-up (GEE) was significantly higher in TCV group (ß=-4.42, p=0.001). MDI scores did not show the adverse association with neonatal TCV exposure.

[Lack of association between thimerosal-containing vaccines and autism]. / Brak zwiazku miedzy ekspozycja na szczepionki zawierajace tiomersal i rozwojem autyzmu u dzieci.

In Poland, administered childhood vaccines still contain thimerosal as a preservative. Despite the access to mercury free formulas, the most of children are still vaccinated by thimerosal-containing vaccines (TCV) owing to economical reasons. That circumstances caused the rising discussion on potential harmful influence of TCVs on children health. The objective of this analysis was to determine an association of TCVs exposure with the risk of autism. Study population included 96 cases diagnosed with childhood or atypical autism and 192 controls matched individually by year of birth, gender, and physician's practice. Data on autism diagnose and vaccination history were from GPs. Data on the other possible autism risk factors were collected from mothers. Conditional logistic regression was used to assess the risk of autism due to TCVs exposure. No significant association was found between TCVs exposure and autism. After adjusting to potential confounders, odds ratios of the risk of autism developing for infants vaccinated with TCVs were 1.52 (95% CI: 0.29-11.11) for doses 12.5-87.5 microg, 2.78 (95% CI: 0.29-11.11) for 100-137.5 microg and 1.97 (95% CI: 0.37-18.95) for these exposed > or = 150 microg. Our study revealed no evidence of an association between TCVs and autism.

[The parents opinion on the risk factors of autism in their children]. / Opinie rodziców na temat czynników, które mogly spowodowac rozwój autyzmu u ich dzieci.

The mothers' opinions about the reasons of their children autism have been collected during case-control study on risk factors of that disease. The relationship between different opinions and mothers' characteristics have been analyzed. The categories of autism reasons mentioned by mothers have been related to the current knowledge about the risk factors of disease.