ABSTRACT
The hypoglycemic effect of gliclazide is mainly due to its action on ATP stimulated K+ channels, but the calcium ionophoretic effect of this drug may also be involved in its physiological properties. Using 1H NMR we demonstrated the antiionophoretic effect of nifedipine and diltiazem. We attempted to verify whether this in vitro interaction also occurs in vivo. A clinical trial, was performed on patients treated concomitantly with gliclazide and nifedipine or diltiazem. Results showed that no in vivo interaction occurred. The discrepancy between in vivo and in vitro results may be explained by a too weak plasma concentration in the case of nifedipine and by a large plasma protein binding in the case of diltiazem.
Subject(s)
Calcium/metabolism , Diltiazem/pharmacology , Gliclazide/pharmacology , Nifedipine/pharmacology , Adenosine Triphosphate , Aged , Binding Sites , Blood Glucose/analysis , Calcimycin/chemistry , Calcimycin/pharmacology , Chromatography, Gas , Chromatography, High Pressure Liquid , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/drug therapy , Drug Interactions , Female , Humans , Insulin/blood , Magnetic Resonance Spectroscopy , Male , Middle Aged , Potassium Channels/drug effectsABSTRACT
Fifty-four amines used as antioxidants and/or antiozonants for elastomers were separated on silica gel thin-layer plates with benzene and benzene-ethyl acetate-acetone (100:5:1) as developing solvents and N-chloro-2,6-dichloro-p-benzoquinone monoimine in buffered alkaline medium as spray reagent. Benzene-n-hexane (50:50) was used to separate diheptyl- and dioctyldiphenylamines. The active agents of several imprecisely defined products were identified and the relationships between some mixtures were elucidated. The method highlights the numerous minor components of these very impure industrial compounds. Some theoretical correlations between the chemical structures, the RF values and the colours of the spots were also established.
