ABSTRACT
A conserved domain, called GGDEF (referring to a conserved central sequence pattern), is detected in many procaryotic proteins, often in various combinations with putative sensory-regulatory components. Most sequenced bacterial genomes contain several different GGDEF proteins. The function of this domain has so far not been experimentally shown. Through genetic complementation using genes from three different bacteria encoding proteins with GGDEF domains as the only element in common, we present genetic data indicating (a) that the GGDEF domain is responsible for the diguanylate cyclase activity of these proteins, and (b) that the activity of cellulose synthase in Rhizobium leguminosarum bv. trifolii and Agrobacterium tumefaciens is regulated by cyclic di-GMP as in Acetobacter xylinum.
Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Cyclic GMP/analogs & derivatives , Phosphorus-Oxygen Lyases/chemistry , Phosphorus-Oxygen Lyases/metabolism , Amino Acid Motifs , Bacterial Proteins/metabolism , Cellulose/metabolism , Cyclic GMP/metabolism , Escherichia coli Proteins , Gene Expression Regulation, Bacterial , Phosphorus-Oxygen Lyases/genetics , Plasmids/genetics , Protein Structure, Tertiary , Repressor Proteins/chemistry , Repressor Proteins/genetics , Repressor Proteins/metabolism , Rhizobium/enzymology , Rhizobium/geneticsABSTRACT
Three herbicide 2,4-D metabolizing bacterial strains were isolated from three independent soil samples of Estonia. The strains, although belonging to various species, contain 2,4-D degradative plasmids with identical restriction patterns. pEST4001 is a 78 kb conjugative plasmid. All Pseudomonas putida PaW340 2,4-D+ transconjugants obtained a 70 kb plasmid pEST4011 - a deletion derivative of the pEST4001. The restriction patterns of the plasmids mentioned above are considerably different from those of the other 2,4-D plasmids pJP4 and pRC10 reported previously.
