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1.
J Food Prot ; 87(8): 100317, 2024 Jun 13.
Article in English | MEDLINE | ID: mdl-38878899

ABSTRACT

The 2021 FSIS Stabilization Guidelines for Meat and Poultry Products (Appendix B) Option 1.2 limits Phase 1 cooling from 48.8 to 26.7 °C in uncured meats to 1 h. However, this time restriction is impractical to achieve in large-diameter whole-muscle products. The objective of this study was to compare the inhibitory effect of commercial dry vinegars (DVs) and cultured sugar-vinegar blends (CSVs) on Clostridium perfringens and Bacillus cereus in uncured beef and poultry products during extended cooling. Treatments (beef: 72-73% moisture, pH 6.2-6.3, 0.85-0.95% NaCl; turkey: 76-77% moisture, pH 6.5-6.7, 1.3-1.6% NaCl) included Controls without antimicrobials, and four DV and four CSV, each tested at 0.75 and 1.25%. Batches were inoculated with 2.5-log C. perfringens or B. cereus spores, vacuum-packaged, and cooked to 73 °C. Packages were cooled from 48.8 to 27 °C (Phase 1) in 3, 4, or 5 h; Phase 2 (27-12.8 °C) and Phase 3 (12.8-4 °C) were standardized for 5-h cooling each. Pathogens were enumerated on selective agar in triplicate samples assayed at precook, postcook, and at the end of Phase 1, 2, and 3 cooling. Experiments were conducted twice. B. cereus did not grow (<0.5-log increase) in any treatment when Phase 1 cooling was extended to 5 h. C. perfringens grew rapidly (2.5 to >4.5 log) in Control treatments when Phase 1 cooling was extended to ≥3 h. All 1.25% DV ingredients limited C. perfringens growth to ≤1-log when Phase 1 cooling was extended to 3 h but supported a >1-log increase when Phase 1 cooling was extended to 5 h. All 1.25% CSV inhibited growth under 3-h Phase 1 cooling; 1.25% CSV-A and ≥0.75% CSV-D inhibited growth in turkey during 5-h Phase 1 cooling, but inhibition with 1.25% CSV-C was inconsistent in beef. This study revealed that formulating uncured meats with 1.25% DV or certain CSV can extend Phase 1 cooling to 3 h. Although all ingredients inhibited growth when used at 0.75% or greater compared to a control, greater variability of inhibition was observed among CSV than for DV.

2.
J Int Neuropsychol Soc ; 28(2): 123-129, 2022 02.
Article in English | MEDLINE | ID: mdl-33896436

ABSTRACT

OBJECTIVE: We examined parent- and adolescent-reported executive functioning (EF) behaviors following pediatric traumatic brain injury (TBI) in the context of Online Family Problem-Solving Therapy (OFPST) and moderators of change in EF behaviors. METHOD: In total, 274 families were randomized to OFPST or an internet resource comparison group. Parents and adolescents completed the Behavior Rating Inventory of Executive Function at four time points. Mixed models were used to examine EF behaviors, assessing the effects of visit, treatment group, rater, TBI severity, age, socioeconomic status, and family functioning. RESULTS: Parents rated their adolescents' EF as poorer (F(3,1156) = 220.15, p < .001; M = 58.11, SE = 0.73) than adolescents rated themselves (M = 51.81, SE = 0.73). Across raters, EF behaviors were poorer for adolescents whose parents had less education (F(3,1156) = 8.60, p = .003; M = 56.76, SE = 0.98) than for those with more education (M = 53.16, SE = 0.88). Age at baseline interacted with visit (F(3,1156) = 5.05, p = .002), such that families of older adolescents reported improvement in EF behaviors over time. Family functioning also interacted with visit (F(3, 1156) = 2.61, p = .049), indicating more improvement in EF behaviors over time in higher functioning families. There were no effects of treatment or TBI severity. CONCLUSION: We identified a discrepancy between parent- and adolescent-reported EF, suggesting reduced awareness of deficits in adolescents with TBI. We also found that poorer family functioning and younger age were associated with poorer recovery after TBI, whereas adolescents of parents with less education were reported as having greater EF deficits across time points.


Subject(s)
Brain Injuries, Traumatic , Executive Function , Adolescent , Brain Injuries, Traumatic/complications , Child , Humans , Parents , Problem Solving , Randomized Controlled Trials as Topic
3.
Front Rehabil Sci ; 3: 1089079, 2022.
Article in English | MEDLINE | ID: mdl-36824180

ABSTRACT

Objective: We describe the clinical implementation in North America of Teen Online Problem Solving (TOPS), a 10+ session, evidence-based telehealth intervention providing training in problem-solving, emotion regulation, and communication skills. Methods: Twelve children's hospitals and three rehabilitation hospitals participated, agreeing to train a minimum of five therapists to deliver the program and to enroll two patients with traumatic brain injuries (TBI) per month. Barriers to reach and adoption were addressed during monthly calls, resulting in expansion of the program to other neurological conditions and extending training to speech therapists. Results: Over 26 months, 381 patients were enrolled (199 TBI, 182 other brain conditions), and 101 completed the program. A total of 307 therapists were trained, and 58 went on to deliver the program. Institutional, provider, and patient barriers and strategies to address them are discussed. Conclusions: The TOPS implementation process highlights the challenges of implementing complex pediatric neurorehabilitation programs while underscoring potential avenues for improving reach and adoption.

4.
Child Neuropsychol ; 26(5): 711-720, 2020 07.
Article in English | MEDLINE | ID: mdl-31833455

ABSTRACT

Although a key goal of neuropsychological evaluation is to improve patient outcomes, research regarding changes in child functioning following neuropsychological evaluation is very limited. This project examined changes from before neuropsychological evaluation to several months afterward in parent report of their child's functioning and ability to participate across home, school, and community settings. Fifty-one parents of children and adolescents receiving their first outpatient neuropsychological evaluation completed questionnaires prior to initial parent interview and following the final report (of n = 162 who were mailed the follow-up questionnaire). Parents rated their perception of the severity of their child's problems, their child's ability to participate, and satisfaction with the child's ability to participate across school, home, and community domains. Analysis utilized MANOVA with bootstrapping to obtain standard errors and false discovery rate to control Type-1 errors. Parents reported a significant decrease in the severity of problems related to academic progress (p = .034) and overall severity of problems (p = .028) at follow-up. There was no significant change in the rated severity of the child's problems with peers or family. Parents reported significant increases in their child's ability to participate, and parent satisfaction with child participation, in school (ps<.001), at home (ps<.01), and in the community (ps<.001). Although this observational study cannot definitively show cause-and-effect, findings support the utility of pediatric neuropsychological evaluations in facilitating child functioning. These findings dovetail with prior work suggesting that such evaluations can increase parents' understanding of their child, awareness of care options, and efficacy in pursuing those options.


Subject(s)
Family/psychology , Neuropsychological Tests/statistics & numerical data , Personal Satisfaction , Adolescent , Adult , Child , Female , Humans , Male , Motivation , Schools , Surveys and Questionnaires
5.
Clin Neuropsychol ; 33(4): 743-759, 2019 05.
Article in English | MEDLINE | ID: mdl-30821626

ABSTRACT

OBJECTIVE: The handful of studies examining parent satisfaction after pediatric neuropsychological evaluations have focused on post-evaluation appraisals. By examining parent experiences across the course of their child's evaluation, this study aimed to provide important insights into how and when parents experience changes in knowledge, understanding of care options, and efficacy during evaluation process. METHOD: Parents of youth receiving neuropsychological evaluation completed questionnaires at four time points (prior to evaluation [n = 363], day of testing [n = 300], prior to feedback [n = 250], and post-report [n = 99]). Parents rated aspects of their knowledge and efficacy regarding their child's functioning. Parents also rated their perception of the neuropsychologist, medical provider, and school along the same domains. The resulting longitudinal data were analyzed using structural equation modeling and ANCOVA. Although primary analyses focused on the entire sample, differences between first-time evaluations and re-evaluations were also examined. RESULTS: Families receiving an initial evaluation showed lower ratings in knowledge, awareness of options, and efficacy at the beginning of the evaluation and a significant increase in ratings by the end of the evaluation. Families returning for re-evaluation showed higher initial ratings that changed comparatively little during the evaluation. Parents receiving initial evaluations also perceived increased knowledge of their child by medical providers and school. CONCLUSION: The study supports the clinical assumption that parents gain knowledge about their child and treatment options during a neuropsychological evaluation. The difference between initial and re-evaluation warrants further study. Studying the process and experience of neuropsychological evaluation may provide more nuanced findings than post hoc satisfaction measures.


Subject(s)
Neuropsychological Tests/standards , Child , Female , Humans , Male , Personal Satisfaction , Surveys and Questionnaires
6.
Fertil Steril ; 109(4): 665-674, 2018 04.
Article in English | MEDLINE | ID: mdl-29452698

ABSTRACT

OBJECTIVE: To determine whether cleavage anomalies, multinucleation, and specific cellular kinetic parameters available from time-lapse imaging are predictive of developmental capacity or blastocyst chromosomal status. DESIGN: Retrospective analysis of prospectively collected data. SETTING: Single academic center. PATIENT(S): A total of 1,478 zygotes from patients with blastocysts biopsied for preimplantation genetic screening were cultured in the EmbryoScope. INTERVENTION(S): Trophectoderm biopsy. MAIN OUTCOME MEASURE(S): Embryo dysmorphisms, developmental kinetics, and euploidy. RESULT(S): Of the 767 biopsied blastocysts, 41.6% (95% confidence interval [CI], 38%-45%) were diagnosed as euploid. Individual dysmorphisms such as multinucleation, reverse cleavage, irregular chaotic division, or direct uneven cleavage were not associated with aneuploidy. Direct uneven cleavage and irregular chaotic division embryos did, however, exhibit lower developmental potential. The presence of two or more dysmorphisms was associated with an overall lower euploidy rate, 27.6% (95% CI 19%-39%). Early embryo kinetics were predictive of blastocyst development but not ploidy status. In contrast, chromosomal status correlated significantly with start time of blastulation (tSB), expansion (tEB), and the tEB-tSB interval. A lower euploidy rate, 36.6% (95% CI 33%-42%) was observed with tSB ≥ 96.2 hours, compared with 48.2% with tSB < 96.2 (95% CI 42%-54%). A drop in euploidy rate to 30% (95% CI 25%-37%) was observed in blastocysts with delayed expansion (tEB > 116). The proportion of euploid blastocysts was increased with tEB-tSB intervals of ≤13 hours. A logistic regression model to enhance the probability of selecting a euploid blastocyst was constructed. CONCLUSION(S): Morphokinetics may aid in selection of euploid embryos from a cohort of day 5/6 blastocysts.


Subject(s)
Blastocyst/pathology , Cell Cycle , Cell Nucleus/pathology , Cleavage Stage, Ovum/pathology , Fetoscopy , Ploidies , Time-Lapse Imaging , Zygote/pathology , Adult , Biopsy , Embryo Culture Techniques , Embryonic Development , Female , Genetic Testing , Humans , Kinetics , Pregnancy , Preimplantation Diagnosis/methods , Retrospective Studies
7.
Clin Neuropsychol ; 32(3): 345-367, 2018 04.
Article in English | MEDLINE | ID: mdl-29243544

ABSTRACT

OBJECTIVE: The neuropsychological report is a critical tool for communicating evaluation results to multiple audiences who have varying knowledge about neuropsychology and often have limited ability to review long, complex reports. Considerable time is spent writing these reports and challenges persist related to readability, length/complexity, and billable clinical time (which may be capped by third-party payors or families' ability to pay). METHODS: This quality improvement effort systematically evaluated the redesign of pediatric neuropsychological reports in an outpatient clinic serving primarily medical populations. RESULTS: Revised reports were shorter, with improved readability, structure, and effectiveness in communicating results and recommendations. Improved clinical efficiency was also observed. CONCLUSIONS: We suggest that adaptation to efficient, readable, and effective reports is possible within the practice of neuropsychology. Findings encourage replication in other settings. Through collaboration with key stakeholders, providers can identify their populations' and audience's unique needs and set report targets accordingly. To encourage that practice, we summarize our general process, provide a set of guidelines that can be adapted across multiple settings, and include an appended sample report.


Subject(s)
Communication , Comprehension , Neuropsychological Tests/standards , Neuropsychology/standards , Professional-Patient Relations , Research Report/standards , Adolescent , Child , Delivery of Health Care/methods , Delivery of Health Care/organization & administration , Female , Humans , Male , Neuropsychology/methods , Quality Improvement/standards
8.
Assessment ; 25(7): 929-941, 2018 10.
Article in English | MEDLINE | ID: mdl-27630203

ABSTRACT

This study outlines the development of the Parent Experience of Assessment Scale (PEAS), which is based on principles of Therapeutic Assessment. The study includes pilot testing of a 64-item questionnaire across 134 participants, with psychometric analyses utilizing confirmatory factor analysis. The revised version consists of 24 items across five subscales with appropriate internal consistency reliability (alphas from .76 to .88). The PEAS demonstrates statistically significant relations with general parent satisfaction, with two subscales indicating significant direct effects via structural equation modeling. The PEAS has the potential utility to provide more nuanced clinical and investigative feedback regarding the parent process during child psychological assessment.


Subject(s)
Parents/psychology , Personal Satisfaction , Surveys and Questionnaires , Adolescent , Adult , Child , Child, Preschool , Factor Analysis, Statistical , Female , Humans , Male , Models, Theoretical , Neuropsychological Tests , Psychometrics , Reproducibility of Results
9.
Fertil Steril ; 106(6): 1370-1378, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27565255

ABSTRACT

OBJECTIVE: To identify blastocyst features independently predictive of successful pregnancy and live births with vitrified-warmed blastocysts. DESIGN: Retrospective study. SETTING: Academic hospital. PATIENT(S): Women undergoing a cycle with transfer of blastocysts vitrified using the Rapid-i closed carrier (n = 358). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Clinical pregnancy and live-birth rates analyzed using logistic regression analysis. RESULT(S): A total of 669 vitrified-warmed blastocysts were assessed. The survival rate was 95%. A mean of 1.7 ± 0.5 embryos were transferred. The clinical pregnancy, live-birth, and implantation rates were 55%, 46%, and 43%, respectively. The odds of clinical pregnancy (odds ratio [OR] 3.08; 95% confidence interval [CI], 1.88-5.12) and live birth (OR 2.93; 95% CI, 1.79-4.85) were three times higher with day-5 blastocysts versus slower-growing day-6 vitrified blastocysts, irrespective of patient age at cryopreservation. Blastocysts from multinucleated embryos were half as likely to result in a live birth (OR 0.46; 95% CI, 0.22-0.91). A four -fold increase in live birth was observed if an expanded blastocyst was available for transfer. The inner cell mass-trophectoderm score correlated to positive outcomes in the univariate analysis. The implantation rate was statistically significantly higher for day-5 versus day-6 vitrified blastocysts (50% vs. 29%, respectively). CONCLUSION(S): The blastocyst expansion grade after warming was predictive of successful outcomes independent of the inner cell mass or trophectoderm score. Delayed blastulation and multinucleation were independently associated with lower live-birth rates in frozen cycles. Implantation potential of the frozen blastocysts available should be included in the decision-making process regarding embryo number for transfer.


Subject(s)
Blastocyst/pathology , Blastula/pathology , Cryopreservation , Embryo Transfer , Fertilization in Vitro , Infertility/therapy , Academic Medical Centers , Adult , Chi-Square Distribution , Embryo Culture Techniques , Embryo Implantation , Embryo Transfer/adverse effects , Female , Fertility , Humans , Infertility/diagnosis , Infertility/physiopathology , Live Birth , Logistic Models , Multivariate Analysis , Odds Ratio , Pregnancy , Pregnancy Rate , Retrospective Studies , Risk Factors , Time Factors , Treatment Outcome
10.
Fertil Steril ; 105(2): 275-85.e10, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26522611

ABSTRACT

OBJECTIVE: To determine if the addition of continuous morphokinetic data improves reproductive outcomes when all embryos are cultured in a closed system. DESIGN: Prospective, randomized, controlled study. SETTING: Single academic center. PATIENT(S): A total of 235 patients undergoing fresh autologous IVF cycles with at least four embryos, cultured in the Embryoscope: 116 patients randomized to conventional once-daily morphologic embryo screening (CS) and 119 to additional time-lapse kinetic monitoring (TLM) for selection. INTERVENTION(S): TLM versus CS. MAIN OUTCOME MEASURE(S): Intrauterine clinical pregnancy (CPR) and implantation (IR) rates. RESULT(S): CPR and IR were similar overall (TLM vs. CS, respectively: CPR 68% vs. 63%; IR 51% vs. 45%) and with blastocyst transfers (CPR 74% vs. 67%; IR 56% vs. 51%). CPR with day 5 transfer was threefold higher than day 3 transfer, but group (TLM vs. CS) was not a significant predictor of clinical pregnancy or implantation. Significantly more multinucleation was detected when CS embryos were retrospectively reviewed with the use of TLM (7.0% vs. 35.3%), and multinucleation was independently associated with decreased rates of implantation. Time to the start of blastulation of <100 hours after insemination and the morphokinetic scoring system used in the TLM group were independently associated with implantation. CONCLUSION(S): The addition of time-lapse morphokinetic data did not significantly improve clinical reproductive outcomes in all patients and in those with blastocyst transfers. Absence of multinucleation, timing of blastulation, and morphokinetic score were found to be associated with blastocyst implantation rates. CLINICAL TRIAL REGISTRATION NUMBER: NCT02081859.


Subject(s)
Blastocyst/physiology , Embryo Culture Techniques , Embryo Transfer , Fertility , Fertilization in Vitro , Infertility/therapy , Pregnancy Rate , Time-Lapse Imaging , Adolescent , Adult , Embryo Implantation , Embryo Transfer/adverse effects , Female , Fertilization in Vitro/adverse effects , Humans , Infertility/diagnosis , Infertility/physiopathology , Kinetics , Logistic Models , Morphogenesis , Odds Ratio , Ohio , Pregnancy , Prospective Studies , Risk Factors , Time Factors , Treatment Outcome , Young Adult
11.
Reprod Biol Endocrinol ; 12: 54, 2014 Jun 20.
Article in English | MEDLINE | ID: mdl-24951056

ABSTRACT

BACKGROUND: Time-lapse imaging combined with embryo morphokinetics may offer a non-invasive means for improving embryo selection. Data from clinics worldwide are necessary to compare and ultimately develop embryo classifications models using kinetic data. The primary objective of this study was to determine if there were kinetic differences between embryos with limited potential and those more often associated with in vitro blastocyst formation and/or implantation. We also wanted to compare putative kinetic markers for embryo selection as proposed by other laboratories to what we were observing in our own laboratory setting. METHODS: Kinetic data and cycle outcomes were retrospectively analyzed in patients age 39 and younger with 7 or more zygotes cultured in the Embryoscope. Timing of specific events from the point of insemination were determined using time-lapse (TL) imaging. The following kinetic markers were assessed: time to syngamy (tPNf), t2, time to two cells (c), 3c (t3), 4c ( t4), 5c (t5), 8c (t8), morula (tMor), start of blastulation (tSB); tBL, blastocyst (tBL); expanded blastocyst (tEBL). Durations of the second (cc2) and third (cc3) cell cycles, the t5-t2 interval as well as time to complete synchronous divisions s1, s2 and s3 were calculated. Incidence and impact on development of nuclear and cleavage anomalies were also assessed. RESULTS: A total of 648 embryos transferred on day 5 were analyzed. The clinical pregnancy and implantation rate were 72% and 50%, respectively. Morphokinetic data showed that tPNf, t2,t4, t8, s1, s2,s3 and cc2 were significantly different in embryos forming blastocysts (ET or frozen) versus those with limited potential either failing to blastulate or else forming poor quality blastocysts ,ultimately discarded. Comparison of embryo kinetics in cycles with all embryos implanting (KID+) versus no implantation (KID-) suggested that markers of embryo competence to implant may be different from ability to form a blastocyst. The incidence of multinucleation and reverse cleavage amongst the embryos observed was 25% and 7%, respectively. Over 40% of embryos exhibiting these characteristics did however form blastocysts meeting our criteria for freezing. CONCLUSIONS: These data provide us with a platform with which to potentially enhance embryo selection for transfer.


Subject(s)
Blastocyst/cytology , Blastomeres/cytology , Ectogenesis , Embryo, Mammalian/cytology , Morula/cytology , Zygote/cytology , Adult , Blastocyst/classification , Blastocyst/pathology , Blastomeres/pathology , Cell Nucleus Division , Cell Proliferation , Cryopreservation , Embryo Culture Techniques , Embryo, Mammalian/pathology , Female , Humans , Infertility, Female/therapy , Infertility, Male , Male , Microscopy, Video , Morula/pathology , Ohio/epidemiology , Ovulation Induction , Pregnancy , Pregnancy Rate , Retrospective Studies , Sperm Injections, Intracytoplasmic , Time-Lapse Imaging , Zygote/pathology
12.
Brain Inj ; 27(9): 1056-62, 2013.
Article in English | MEDLINE | ID: mdl-23781827

ABSTRACT

OBJECTIVE: To investigate the relationship between injury severity variables, particularly time to follow commands (TFC) and long-term functional outcomes in paediatric traumatic brain injury (TBI). METHODS AND PROCEDURE: Participants included 40 children with moderate-to-severe TBI discharged from inpatient rehabilitation. Measures of severity were initial Glasgow Coma Scale score, TFC, duration of Post Traumatic Amnesia (PTA) and total duration of impaired consciousness (TFC + PTA). Functional outcome was measured by age-corrected Functional Independence Measure for Children (WeeFIM®) scores at 1-year after discharge. RESULTS: Correlations indicated that injury severity variables (TFC, PTA and TFC + PTA) were all associated with functional outcome. Regression analyses revealed that TFC and TFC + PTA similarly accounted for 49% or 47% of the variance, respectively, in total WeeFIM® score. Thirty-seven of 40 children had good outcome; of the three children with TFC >26 days, two had poor outcome. CONCLUSION: PTA and TFC + PTA do not provide a benefit over TFC alone for prediction of long-term outcome and TFC is identified earlier in the recovery course. TFC remains an important predictor of functional outcome 1-year after discharge from inpatient rehabilitation after paediatric TBI.


Subject(s)
Brain Injuries/epidemiology , Disability Evaluation , Activities of Daily Living , Adolescent , Age of Onset , Brain Injuries/physiopathology , Brain Injuries/rehabilitation , Child , Child, Preschool , Female , Glasgow Coma Scale , Humans , Male , Predictive Value of Tests , Prognosis , Rehabilitation Centers/statistics & numerical data , Severity of Illness Index , Time Factors , United States/epidemiology
13.
Reprod Biol Endocrinol ; 11: 41, 2013 May 15.
Article in English | MEDLINE | ID: mdl-23672340

ABSTRACT

BACKGROUND: The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000°C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of this device for the cryopreservation of cleavage, as well as blastocyst stage human embryos is also analyzed. We further compare outcomes to those achieved with the cryoloop, an "open" vitrification system routinely used in our laboratory. METHODS: Human embryos were vitrified at either the 8-10 cell stage or else the blastocyst stage. The vitrification protocol was: 7.5% DMSO/7.5% ethylene glycol (EG) (2-3 min) followed by incubation in 15% DMSO /15% EG (45 sec) before loading on the vitrification carrier. Cryoprotectant was removed during warming by sequential washes in 0.25 M and 0.125 M sucrose in culture medium. Clinical outcome data for frozen cycles between January 2011 and August 2012 were stratified according to carrier and cell stage. The student t-test and chi square test were used to compare results. P value of < 0.05 was considered significant. RESULTS: A total of 486 vitrified-warmed embryos were assessed and 92% of them were transferred. The clinical pregnancy rate (CPR) and implantation rate (IR) with Rapid-i vitrified blastocysts were 59% and 49%, versus 47% and 37%, respectively for cleavage stage embryos. This was not statistically different from results with the cryoloop vitrified blastocysts (CPR 46%, IR 38%) nor the cleavage stage vitrified embryos (CPR 49%, IR 35%). To date, there have been 31 deliveries of 34 healthy infants from Rapid-i vitrified embryos, with another 12 pregnancies still on-going. CONCLUSIONS: The Rapid-i offers an excellent alternative to existing open vitrification devices for embryo cryopreservation at the 8-10 cell stage as well as the blastocyst stage. Use of this type of "closed" sealed system that prevents direct contact between the embryos and liquid nitrogen reduces the potential risk of sample cross-contamination or infection. These preliminary data and live birth outcomes have paved the way toward transitioning to a closed vitrification system in our own IVF program.


Subject(s)
Cryopreservation/instrumentation , Cryopreservation/methods , Embryo, Mammalian/physiology , Vitrification , Adult , Blastocyst/drug effects , Blastocyst/physiology , Chi-Square Distribution , Cleavage Stage, Ovum/drug effects , Cryoprotective Agents/pharmacology , Dimethyl Sulfoxide/pharmacology , Embryo Implantation , Embryo Transfer , Embryo, Mammalian/drug effects , Embryo, Mammalian/embryology , Ethylene Glycol/pharmacology , Female , Fertilization in Vitro/methods , Humans , Pregnancy , Pregnancy Rate , Reproducibility of Results , Sucrose/pharmacology
14.
J Pers Assess ; 94(6): 571-85, 2012.
Article in English | MEDLINE | ID: mdl-22475356

ABSTRACT

This case study provides an in-depth example of a comprehensive therapeutic assessment with an adolescent (TA-A) and his parents. The TA-A addressed parental concerns about their son's drug experimentation as well as the adolescent's own private questions about his distinctiveness from others, all set against a backdrop of ongoing parental conflict and poor communication. The TA-A process and how it is specifically tailored to balance the needs of adolescents and their parents is discussed. Subsequently, each step of TA-A is illustrated through the case study. Research findings at the conclusion of the assessment and at follow-up indicated significant decreases in internalizing symptomology and school problems, increases in self-esteem and self-reliance, and improved family functioning as reported by the adolescent. At follow-up, the father spoke of developing a more assertive parenting approach and successful follow-through on recommendations. This case study provides a template for clinicians interested in conducting TA-A.


Subject(s)
Interpersonal Relations , Personality Assessment , Psychotherapy/methods , Substance-Related Disorders/rehabilitation , Adolescent , Assertiveness , Education , Family Conflict/psychology , Humans , Individuation , Internal-External Control , Male , Parent-Child Relations , Self Concept , Substance-Related Disorders/psychology , Underachievement
15.
J Assist Reprod Genet ; 29(5): 375-9, 2012 May.
Article in English | MEDLINE | ID: mdl-22391825

ABSTRACT

OBJECTIVE: To describe a new technique for freezing individually isolated spermatazoa from testicular biopsies, epididymal aspirates and oligospermic semen samples METHODS: Samples were evaluated for the presence of motile sperm before cryopreservation. Motile or twitching sperm were isolated with an ICSI needle for single sperm cryopreservation. Selected sperm were loaded on the High Security Straw (HSV; Irvine Scientific; Irvine,CA), in ~0.5 µl of fluid to facilitate recovery. The sample was also frozen using conventional methodology in cryovials (100-1000 µl aliquots). In both freezing techniques, the samples were slow cooled. Test-yolk buffer-glycerol (Irvine) was used as the cryoprotectant. Test-thaws were performed to assess sperm recovery and motility. RESULTS: Six men with azoospermia had single sperm cryopreservation, as well as freezing aliquots of their testicular or epididymal sperm in traditional cryovials. In addition, two men with oligospermia also had individual sperm selected and frozen. In all 8 cases, the ~0.5 µl of fluid containing sperm was quite easily unloaded from the HSV straw during thawing. The percent sperm recovery ranged from 33% to 100%. Motility was evident in all but one sample. In six cases, the sperm were used for intracytoplasmic sperm injection of mature oocytes. Fertilization occurred in all but one case. In this study, we report the first clinical pregnancy with this technique. This pregnancy was remarkable in that a single motile sperm identified and selected in the initial testicular preparation was successfully frozen. We were able to subsequently recover this sperm, fertilize an oocyte and the resultant embryo gave rise to a live birth. The methodology described in this preliminary report offers a new modality for sequestering small numbers of sperm. It may be particularly useful in cases involving severe impairment of spermatogenesis, where extensive screening may be necessary to find a few viable sperm.


Subject(s)
Cryopreservation/methods , Semen Preservation/methods , Spermatozoa , Cryoprotective Agents , Epididymis , Female , Fertilization in Vitro/methods , Humans , Male , Oligospermia , Oocytes , Pregnancy , Sperm Injections, Intracytoplasmic/methods , Sperm Motility/physiology , Spermatogenesis , Testis
16.
J Reprod Med ; 54(9): 548-52, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19947031

ABSTRACT

OBJECTIVE: To describe our Partnership for Families Program, which was established to provide second in vitro fertilization (IVF) cycles for couples who pay for one IVF cycle, do not get pregnant and cannot afford a second IVF cycle. In addition, this program provides funding for fertility-sparing procedures for financially needy cancer patients. STUDY DESIGN: Retrospective description of the Partnership for Families' first 5 years of operation. RESULTS: In its 5 years of operation, the Partnership for Families Program has provided 137 infertile couples with a second IVF cycle, resulting in 68 ongoing or delivered pregnancies. It has also provided funding for 19 fertility-sparing procedures for cancer patients. CONCLUSION: Because of the high costs of IVF, alternative funding sources, specifically philanthropy, must be explored to provide increased access to IVF. The Partnership for Families Program, started by patients in a single practice, has in 5 years provided over 151 infertile and cancer patients IVF or egg-freezing cycles that they otherwise could not have afforded. This is a program that can be emulated by other fertility centers.


Subject(s)
Fertilization in Vitro/economics , Fund Raising , Female , Humans , Infertility/economics , Infertility/therapy , Pregnancy , Program Evaluation , Retrospective Studies , Treatment Outcome
17.
J Pers Assess ; 91(2): 108-20, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19205932

ABSTRACT

We present a case study of a child's psychological assessment using the methods of Therapeutic Assessment (TA). The case illustrates how TA can help assessors understand the process and structure of a family by highlighting how maladaptive family processes and interactions impact a child's development. It also illustrates how TA with a child can serve as a family intervention. In this case, it became apparent that the child's social difficulties were significant, not minor as initially reported by the parents, and were rooted in an insecure attachment, underlying depression, an idiosyncratic view of the world, and longing for attention, all of which were hidden or expressed in grandiose, expansive, and off-putting behaviors. In addition, the familial hierarchy was inverted; the parents felt ineffective and the child felt too powerful, leading to enhanced anxiety for the child. Intervention throughout, punctuated by the family session and feedback sessions, allowed the parents to develop a new "story" about their child and for the child to experience a new sense of safety. Following the TA, the parents and child indicated high satisfaction, enhanced family functioning, and decreased child symptomatology. Subsequent family therapy sessions allowed the family to further implement the interventions introduced in the TA.


Subject(s)
Behavior Therapy/methods , Child Behavior/psychology , Depression/therapy , Parent-Child Relations , Parents/psychology , Child , Child Development , Communication Barriers , Depression/diagnosis , Female , Humans , Personality Assessment , Self Concept , Treatment Outcome
18.
J Pers Assess ; 90(6): 547-58, 2008 Nov.
Article in English | MEDLINE | ID: mdl-18925495

ABSTRACT

Including a family session in a child assessment can significantly advance the assessor's and parents' understanding of the child's problems and enhance the likelihood that parents will follow through on recommendations after the assessment. A family session allows the assessor to observe the child in the family context, test systemic hypotheses, better understand the meaning of individual test results, and try out possible interventions. A family session may also help parents see systemic aspects of their child's problems, help the child feel less blamed, foster positive experiences among family members, and offer the family a glimpse of family therapy. We describe methods and techniques for structuring family sessions and offer guidance on preparing for and conducting such sessions depending on one's case conceptualization. Detailed case examples illustrate each technique and demonstrate the immediate and subsequent impact of family sessions as well as their therapeutic value. We also address common clinical and pragmatic issues.


Subject(s)
Child Behavior Disorders/therapy , Family , Goals , Personality Assessment , Psychology, Child , Child, Preschool , Humans
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