[The effectiveness of complex therapy using the injectable form of chondroitin sulfate and sodium hyaluronate with osteoarthritis of the knee joint].

AIM: The study on the effectiveness of complex therapy for osteoarthritis (OA) of the knee joint was conducted in real clinical practice. MATERIALS AND METHODS: The survey involved 125 patients aged fr om 50 to 70 years (25 men and 100 women) with a diagnosis of knee joint OA (the III roentgenologic Kellgren-Lawrence stage).The average age of the patients was 62±3.21, the average duration of the disease - 9.4±2.8 years. Patients were randomly assigned to three groups of 35 people, the control group had 20 patients. Group 1 patients received non - steroidal anti - inflammatory drugs (NSAIDs) + Injectran(Chondroitin sulfate) 200 mg intramuscularly (I.M.) every other day No. 25.In group 2, patients received NSAIDs + Fermatron 1% 2 ml with an interval of 7 days intra - articularly (I.A.) No. 3. In group 3 - NSAIDs + Injectran 200 mg (I.M.) every other day No. 25 + Fermatron 1% 2 ml with an interval of 7 days (I.A.) No. 3. In the control group (20 people), patients received only NSAIDs. Evaluation of the symptoms was carried out using the WOMAC index before the start of thetherapy, after 8 and 12 weeks of treatment. The intensity of pain while walking was estimated on a visual analogue scale. RESULTS: In the groups that received Injectran (I; group 1) or Fermatron (F; group 2), the dynamics of pain while walking reduction was comparable and had slightly more than 30% in both groups, the figures are reliable in comparison withinitial data (p.

[Role of the electron acceptor properties of lysozyme and its substrate inhibitors in photosensitized electron transport in their complexes by the EPR method]. / Izuchenie roli élektronoaktseptornykh svoistv lizotsima i ego substrat-ingibitorov v fotosensibilizirovannom perenose élektronov v ikh kompleksakh metodom EPR.

The nature of electron-acceptor groups in the system of lysozyme with its substrate-inhibitors has been studied in a wide range of pH values by the method of photosensitized electron transfer. In the lysozyme molecule disulphide bonds and peptide groups are the electron--acceptor groups. The nature of radicals in irradiated lysozyme depends on pH. At complex-formation of lysozyme with oligomeres of N-acetylglucosamine the electron transfer from the enzyme molecule to N-acetyl group of the substrate-inhibitor molecule is realized. Under conditions ruling out complex-formation of lysozyme with the inhibitors (N-acetylglucosamine and its dimer) the electrons are localized on disulphide bonds of the protein molecules at alkaline pH and at pH less than or equal to 3 the radicals are observed which are due to the remove of hydrogen atom from the Calpha-atom of the protein polypeptide chain.

[Localization of unpaired electrons in molecules of the substrate inhibitors of lysozyme. II. Oligosaccharides]. / Issledovanie lokalizatsii nesparennykh élektronov v molekulakh substrat-ingibitorov lizotsima. II. Oligosakharidy.

It has been shown by the method of electron photosensitized transfer that N-acetyl group is the main electron-acceptor group in oligomeres of N-acetyl glucose amine (AGA). In n-AGA molecules (n-3.5) interacting with an electron radical products of the breakage of glycoside bond are observed, their concentration rising with an increase of the chain length. The ionic strength does not affect the photosensitized transfer of the electron in chitin oligomeres. Formation of paramagnetic centres in the molecule penta-AGA depends on the medium pH.

[Localization of unpaired electrons in molecules of lysozyme substrate-inhibitors. I. N-acetylglucosamine]. / Issledovanie lokalizatsii nesparennykh élektronov v molekulakh substrat-ingibitorov lizotsima. I. N-atsetilgliukozamin

Electron structure of N-acetylglucoseamine molecule and its ion-radicals has been studied by quantum-chemical methods CNDO2 and JNDO, and by ESR as well. N-acetyl group is shown to be the only electron acceptor group of AGA molecule. Phototransformations of paramagnetic centres are studied at different pH values.