ABSTRACT
For the determination of haemodynamic indices during the period of in adaptation in children there was examined 271 schoolboy from the gymnasium of the city of Krasnoyarsk There was studied the dynamics of haemodynamic indices in the course of adaptation, there were detected the degree of influence of haemodynamic indices on the process of adaptation, there were revealed features of haemodynamic indices in children with the various forecast of a course of adaptation. Children with the unfavorable forecast of adaptation to school had according to data of cardiorhythmography (CRG) low values of power in a range of slow waves during the period of restoration of the orthostatic test that testifies to the raised tone of sympathetic nervous system. There were established the correlation interrelationships of the forecast of adaptation of children to school with changed (raised, lowered) level of arterial pressure and Robinson's index value.
Subject(s)
Adaptation, Physiological/physiology , Hemodynamics/physiology , Schools , Students , Sympathetic Nervous System/physiology , Child , Female , Humans , MaleABSTRACT
We have developed methods for producing recombinant proteins of Noggin family (Noggin1 and Noggin2 of the Xenopus laevis frog) that can interact with BMP factors of TGF-beta superfamily. The genetic constructs which allow one to effectively obtain Noggin1 and Noggin2 from synthetic mRNA microinjected into Xenopus laevis early embryos, as well as in the prokaryotic expression system, were generated. The obtained proteins contain three Myc-tag epitopes on their N-terminus. This allow one to compare the expression levels of Noggin1 and Noggin 2 constructs, to purify them on the affine immunosorbent and to show the activity of Noggin proteins by analyzing their ability to bind BMP4 factor TGF-beta surperfamily by co-immunoprecipitation.
Subject(s)
Carrier Proteins/genetics , Embryonic Development/genetics , Intracellular Signaling Peptides and Proteins/genetics , RNA, Messenger/genetics , Recombinant Proteins/genetics , Xenopus Proteins/genetics , Amino Acid Sequence , Animals , Bone Morphogenetic Protein 4/metabolism , Carrier Proteins/isolation & purification , Carrier Proteins/metabolism , Gene Expression Regulation, Developmental , Intracellular Signaling Peptides and Proteins/isolation & purification , Protein Binding , RNA, Messenger/chemical synthesis , RNA, Messenger/metabolism , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Signal Transduction , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/metabolism , Xenopus Proteins/isolation & purification , Xenopus laevis/genetics , Xenopus laevis/metabolismABSTRACT
It has been shown that slight sinusoidal fluctuations of temperature, salinity, and oxygen concentration optimize the growth and energy metabolism of the crustacean Leander modestus, mollusks Pila sp., and hydrophyte Elodea canadensis. Under the effect of temperature fluctuations, the growth of E. canadensis accelerates, and the rate of photosynthesis increases.
