Subject(s)
Collagen/chemistry , Collagen/radiation effects , Extracellular Matrix/radiation effects , Fibrillar Collagens/radiation effects , Hot Temperature/adverse effects , Infrared Rays/adverse effects , Lasers/adverse effects , Animals , Calorimetry , Fibrillar Collagens/chemistry , Intervertebral Disc/radiation effects , Rabbits , Temperature , Tomography, Optical CoherenceABSTRACT
It has been shown by biochemical analysis and differential scanning calorimetry that the connective tissue formed around a transplant as a result of sclero-reinforcing interference (capsula) is similar to intact sclera. The main component of newly formed capsules is collagen I whose fibers have a perfect structure and the amount of cross-links sufficient to provide normal thermomechanical properties. A fraction of collagen having thermally labile "immature" cross-links in capsules formed around the transplant impregraned with Panaxal has been detected by differential scanning calorimetry. It was suggested that fibroblasts in tissues of these capsules have a high synthetic activity.
Subject(s)
Connective Tissue/chemistry , Ethers/chemistry , Prostheses and Implants , Sclera/chemistry , Sclera/surgery , Animals , Calorimetry, Differential Scanning , Collagen Type I/analysis , Fibroblasts/chemistry , Hot Temperature , Rabbits , TemperatureSubject(s)
Cartilage/chemistry , Collagen Type II/chemistry , Temperature , Animals , Cattle , Chymotrypsin/chemistry , Protein Denaturation , Trypsin/chemistryABSTRACT
Cartilaginous and connective tissues were exposed to a moderate-intensity IR laser treatment with IR radiometric temperature control. The isotherms of water sorption by dry preparations of intact and laser-treated tissues were obtained and calculated in terms of well known models. It was shown that the sorption capacity of a monolayer of tissues decreases with increasing temperature during laser treatment. Changes in the supramolecular structure of the extracellular matrix of tissues (loss of regularity) upon IR laser exposure are discussed.