A canine model to evaluate the effect of exercise intensity and duration on olfactory detection limits: the running nose.

Detection canines serve critical roles to support the military, homeland security and border protection. Some explosive detection tasks are physically demanding for dogs, and prior research suggests this can lead to a reduction in olfactory detection sensitivity. To further evaluate the effect of exercise intensity on olfactory sensitivity, we developed a novel olfactory paradigm that allowed us to measure olfactory detection thresholds while dogs exercised on a treadmill at two different exercise intensities. Dogs (n = 3) showed a decrement in olfactory detection for 1-bromooctane at 10-3 (v/v) dilutions and lower under greater exercise intensity. Dogs' hit rate for the lowest concentration dropped from 0.87 ± 0.04 when walking at low intensity to below 0.45 ± 0.06 when trotting at moderate intensity. This decline had an interaction with the duration of the session in moderate intensity exercise, whereby dogs performed near 100% detection in the first 10 min of the 8 km/h session, but showed 0% detection after 20 min. Hit rates for high odor concentrations (10-2) were relatively stable at both low (1 ± 0.00) and moderate (0.91 ± 0.04) exercise intensities. The paradigm and apparatus developed here may be useful to help further understand causes of operationally relevant olfactory detection threshold decline in dogs.

Development of an automated human scent olfactometer and its use to evaluate detection dog perception of human scent.

Working Dogs have shown an extraordinary ability to utilize olfaction for victim recovery efforts. Although instrumental analysis has chemically characterized odor volatiles from various human biospecimens, it remains unclear what perceptually constitutes human scent (HS) for dogs. This may be in part due to the lack of methodology and equipment to train and evaluate HS perception. The aims of this research were 1) to develop an automated human scent olfactometer (AHSO) to present HS to dogs in a controlled setting and 2) use the AHSO to evaluate dogs' response to different scented articles and individual components of HS. A human volunteer was placed in a clear acrylic chamber and using a vacuum pump and computer-controlled valves, the headspace of this chamber was carried to one of three ports in a different room. Dogs were trained to search all three ports of the olfactometer and alert to the one containing HS. In Experiment 1 and 2, the AHSO was validated by testing two dogs naïve to HS (Experiment 1) and five certified Search and Rescue (SAR) teams naïve to the apparatus (Experiment 2). All dogs showed sensitivity and specificity to HS > 95% in the apparatus. In Experiment 3, we used a spontaneous generalization paradigm to evaluate generalization from the HS chamber to different scented articles exposed to the same volunteer and to a breath sample. Dogs' response rate to the different scented articles was < 10% but exceeded 40% for the breath sample. In Experiment 4, we replicated this result by re-testing spontaneous generalization to breath and when the volunteer had breath exhausted/removed from the chamber. Dogs' response rate to breath alone was 88% and only 50% when breath was removed. Altogether, the data indicate that exhaled breath is an important and salient component of HS under these conditions.

Semiochemicals from Domestic Cat Urine and Feces Reduce Use of Scratching Surfaces.

Scratching is a natural behavior in cats but can cause damage to household furnishings. In this work, we sought to identify potential semiochemicals in the urine and feces of domestic cats that may modify cat scratching behavior. Sex differences among adult, intact cats were examined for volatile molecules in their urine (n = 7 females, 7 males) and feces (n = 8 females, 10 males) using gas chromatography-mass spectrometry (GC-MS). Males had seven times more 3-Mercapto-3-Methyl Butanol (MMB, p < 0.001) in the urine and 98% more butanoic acid (p = 0.02) in the feces than females. One mL of mineral oil without (i.e., control) or with MMB (0.1 µg/mL) and butanoic acid (100 µg/mL; i.e., treatment), which corresponds to the estimated biological amount in a single elimination from a male cat, were evaluated for their effectiveness in modifying the use of scratching devices by cats. Two identical cardboard standing scratchers, treated with either the control or the solution containing both semiochemicals delivered through a hanging cotton sock were placed side by side in a home/shelter environment. The preference test consisted of exposing individual cats (n = 28) to both scratchers for 20 min and recording the duration and frequency they interacted or scratched each scratcher. The semiochemical solution significantly decreased scratching time (21.19 ± 3.8 vs. 6.08 ± 3.8 s; p < 0.001) and interaction time (31.54 ± 5.9 vs. 12.90 ± 5.9 s; p = 0.0001) and tended to reduce scratching frequency (1.49 ± 0.3 vs. 0.82 ± 0.3 times; p = 0.07) compared with the control solution. The male-representative solution of MMB and butanoic acid was aversive to cats and might have future applications in protecting furniture from the destructive scratching or in modifying behavior of domestic cats.

Part, III: Increasing odor detection performance after training with progressively leaner schedules of odor prevalence.

Prior work has demonstrated canine search behavior and performance declines when challenged with infrequent target odors. The purpose of this study was to evaluate whether performance could be maintained in a low target odor prevalence context by explicitly training dogs through progressively leaner target odor schedules. In Experiment 1, nine control dogs were trained at 90% target prevalence rate. Nine experimental dogs were trained with progressively lower prevalence rates in 10% increments until reaching 20% prevalence with > 85% detection accuracy in the training context. Both groups were tested in the operational context at a 10% target odor prevalence. Experimental dogs had higher accuracy, hit percentage, and shorter search latency in the operational context compared with control dogs. In Experiment 2, twenty-three operational dogs were challenged with a target frequency of 10%, which resulted in 67% accuracy. Control dogs were then trained with 90% target frequency, whereas experimental dogs received a progressively decreasing target rate from 90% to 20%. The dogs were rechallenged with target frequencies of 10, 5, and 0%. Experimental dogs outperformed control dogs (93% vs. 82% accuracy) highlighting the effect of explicit training for infrequent targets.

Explosive odor signature profiling: A review of recent advances in technical analysis and detection.

With the ever-increasing threat of improvised explosive devices (IEDs) and homemade explosives (HME) both domestically and abroad, detection of explosives and explosive related materials is an area of urgent importance for preventing terrorist activities around the globe. Canines are a common biological detector used in explosive detection due to their enhanced olfactory abilities, high mobility, efficient standoff sampling, and optimal identification of vapor sources. While other sensors based on different principles have emerged, an important concept for the rapid field detection of explosives is understanding key volatile organic compounds (VOCs) associated with these materials. Explosive detection technology needs to be on par with a large number of threats including an array of explosive materials as well as novel chemicals used in the manufacture of IEDs. Within this much needed area of research for law enforcement and homeland security applications, several studies have sought to understand the explosive odor profile from a range of materials. This review aims to provide a foundational overview of these studies to provide a summary of instrumental analysis to date on the various types of explosive odor profiles evaluated focusing on the experimental approaches and laboratory techniques utilized in the chemical characterization of explosive vapors and mixtures. By expanding upon these concepts, a greater understanding of the explosive vapor signature can be achieved, providing for enhanced chemical and biological sensing of explosive threats as well as expanding upon existing laboratory-based models for continued sensor development.

A laboratory model of canine search vigilance decrement, II: Noncontingent reward and Pavlovian appetitive stimuli.

Detection dogs have demonstrated reduced performance in operational settings when required to search in an environment where few to no target odors are present. This study's purpose was to increase detection dog accuracy using noncontingent reward (NCR) and Pavlovian stimuli associated with reward. Eighteen dogs were randomly spilt into two groups and received four 40-trial sessions in an operational and training context at 90% odor prevalence (baseline). Following baseline, in the operational context (now at 10% odor prevalence), experimental dogs received an NCR schedule consisting of delivering food rewards at the end of 66% of trials. After the NCR Test, dogs returned to baseline. During baseline, the experimental dogs received 10 days of delayed Pavlovian conditioning to a tone. During the test phase, the conditioned stimulus (tone) was presented to experimental dogs on average every two trials for 30 s in the operational context (now at 10% odor prevalence). Overall, NCR showed a nonsignificant trend for increased responding in the experimental group but tended to increase false alerts; therefore, a permutation of an NCR-like reward schedule may maintain search. The Pavlovian conditioned stimulus didn't decrease timeouts or improve accuracy, but a within-session analysis indicated that the dogs were more likely to time out and less likely to false alert when the tone was on than when it was off.

A laboratory model of canine search vigilance decrement, I.

Previous studies have found that infrequent targets can reduce dogs' vigilance. The purpose of this study was to develop a laboratory model to evaluate the effects of infrequent targets on dogs' search behavior and performance. Dogs (n = 18) were trained to detect smokeless powder in an automated olfactometer in two distinct rooms ("operational" and "training"). During baseline, the dogs received five daily sessions at a high target odor frequency (90%) in both rooms. Subsequently, the frequency of the target odor was decreased to 10% only in the "operational" room but remained at 90% in the training room. Last, the odor prevalence was returned to 90% in both rooms. All dogs showed a significant decrement in detection performance in the operational room when the target odor frequency was decreased but simultaneusly mantained high performance in the training room. This decrement was largely due to decreases in adequate search behavior. All dogs recovered performance when the odor frequency was increased again to 90%. Trial accuracy was associated with tail position, search score, latency, and duration of environmentally directed behaviors. The data show that low target odor prevalence significantly reduced search behavior and performance and that there are behaviors that can be used by handlers to assess their dog's search state.

The effect of repeated testing on judgement bias in domestic dogs (Canis familiaris).

Judgement bias paradigms are increasingly being used as a measure of affective state in dogs. Approach to an ambiguous stimulus is commonly used as a measure of affect, however, this may also be influenced by learning. This study directly measured the impact of learning on a commonly used judgement bias paradigm in the absence of an affective state manipulation. Dogs (N = 15) were tested on a judgement bias task across five sessions. The dogs' latency to approach a bowl placed in one of three ambiguous locations between non-baited (negative) and baited (positive) locations was measured. Results show that session number had a significant effect on the dogs' latencies to reach the ambiguous bowl locations, with post-hoc tests revealing that dogs were significantly slower to approach the locations as the number of sessions increased. Session number also had a significant effect on the number of times the dogs did not approach the bowl within 30 s of being released, with the number of no approaches generally increasing across sessions. When dog identity was included as a fixed effect, a significant effect on latency to approach was found, suggesting that some dogs were consistently faster than others across sessions. To assess whether the paradigm produced repeatable results, Intraclass Correlation Coefficients were used. A low degree of reliability was found between latencies to approach each bowl position across sessions. This study demonstrates that dogs learned that the ambiguous locations were not rewarded with repeated exposures, and that this impacted their responses. We conclude that this judgement bias paradigm may require further consideration if applied across multiple exposures and that repeated results should be interpreted with caution as they are likely impacted by learning.

The use of an intermittent schedule of reinforcement to evaluate detection dogs' generalization from smokeless-powder.

Odor generalization is essential for detection dogs. Regardless of its importance, limited research is available on detection dog odor generalization. The objectives of this study were (1) evaluate the use of an intermittent schedule of reinforcement to assess generalization in dogs and (2) evaluate olfactory generalization from a single exemplar of smokeless powder (SP). Dogs (N = 5) were trained to detect SP in an automated olfactometer under an intermittent schedule of reinforcement where only 60% of correct responses were reinforced. After training, eight non-reinforced probe trials were inserted within a session. A total of 15 testing odors were evaluated across 15 consecutive sessions (one odor/session). Six of the testing odors were control and the remaining testing odors were objects indirectly exposed to SP, objects that contained or were directly exposed to SP, single-base SP and diphenylamine (the main volatile present in the headspace of SP). Dogs' response rate to all testing odors except for the cotton gauzes and t-shirt cloths exposed to the headspace of SP, the simulated IED, and Getxent tubes exposed to direct contact with SP were statistically lower than their response rate of actual SP. The response rate to SP was not different across all 15 testing sessions suggesting that the intermittent schedule of reinforcement, maintained dog motivation and performance. Data show that the outlined method is a good approach to study generalization in detection dogs. These results also highlight dog generalization to SP varieties and associated odors.

Case Study: An Evaluation of Detection Dog Generalization to a Large Quantity of an Unknown Explosive in the Field.

Two explosive detection dogs were deployed to search a suspicious bag, and failed to detect 13 kg of explosive within. The aim of this research was to further evaluate this incident. First, dog teams (N = 7) searched four bags in a similar scenario. One bag contained the same 13 kg of explosive, two bags were blanks, and the other contained the training sample that the agency routinely used for training. All dogs detected the training sample, but most (5/7) did not alert to the 13 kg sample. Subsequently, dogs received two trials in a line up with a 30 g subsample of the explosive to evaluate whether they could generalize to a smaller quantity. Most dogs (6/7) alerted to the subsample at least once. Finally, dogs were trained with the 30 g subsample and later tested with the 13 kg sample. Only three dogs spontaneously generalized to the large sample after training with the small subsample. Dogs' alert rate to the 13 kg sample was improved with training in subsequent trials with the 13 kg sample. This result indicates that explosive detection dogs may not generalize to a target odor at a significantly higher quantity relative to the one used in training, highlighting the importance of conducting such training.

An Automated Canine Line-Up for Detection Dog Research.

Currently, there is a need to develop technology that facilitates and improves detection dog research. The aim of this research was to develop an automated computer-driven olfactory line-up task. The apparatus consisted of three olfactometers. Each olfactometer was equipped with flow meters to regulate air flow and dilution and six solenoid valves connected to odor jars. Each olfactometer generated an odor which was carried to an odor port where the dogs sample it. The olfactometer's valves were activated by a microcontroller, and a Python program was built to control each olfactometer and randomize and balance the odor presentation. Dogs (N = 12) received one or two 40-trial training sessions in a day where they progressed through a series of training phases where they learned to detect and alert to double-base smokeless powder (SP). An "alert" consisted of a 4-s nose hold. This was measured by infrared sensors in the ports. For each trial, the apparatus recorded dogs' search latency, sniff time, port entries, and response. All this information was automatically recorded in a csv file. A photoionization detector (PID) and solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC/MS) were used to evaluate the odor dynamics and to instrumentally verify odor presence and clearance. A control test was conducted at the end of the training to ensure dogs were alerting exclusively to the odorant. All 12 dogs readily learned to operate the apparatus within 23 days, and all exceeded 85% accuracy. Control tests indicated dogs were leveraging only olfactory cues and not unintentional cues such as auditory cues from the apparatus. Analytical data showed that odor was detected in the port immediately after the activation of a valve and that odor clearance occurred immediately after the valve was closed. The apparatus developed was easy to operate by the dogs and allowed substantial data collection using double-blind testing procedures in a very short period at an affordable cost point for research equipment (~$5,000 USD). The apparatus may prove to be a useful research tool to provide optimal odor stimuli control, ensure double-blind conditions, reduce labor, and significantly increase the amount of data collected.

Identification of Faecal Maternal Semiochemicals in Swine (Sus scrofa) and their Effects on Weaned Piglets.

Piglets are attracted to maternal faeces early in life. Thus, the aim of this study was to identify faecal maternal semiochemicals that attract piglets and evaluate their effects on piglets at weaning. Faecal samples were collected from eight sows during gestation and lactation. Faecal semiochemicals were extracted and identified using solid phase extraction and GC/MS. A total of 26 volatiles were present in lactating and gestating sow faeces. Sows secreted no unique semiochemical after farrowing. However, the concentration of skatole and myristic acid were 2.68 and 1.13 times higher after farrowing. A free-choice preference assessment showed that piglets had a preference for a feeder sprayed with a solution containing skatole and myristic acid. No preference was found when feeders were sprayed with skatole and myristic acid individually. The application of skatole and myristic acid to the feeders of weaned pigs significantly reduced piglet aggression by 30% and tended to increase feeding behaviour by 35% the first 24 h post-weaning. These results suggest that skatole and myristic acid might be acting as a multicomponent maternal signal that attracts piglets and has a calming effect at weaning.

Preliminary Study: Depriving Piglets of Maternal Feces for the First Seven Days Post-Partum Changes Piglet Physiology and Performance before and after Weaning.

Coprophagy has been described in piglets although its importance has not been fully assessed. The aim of this study was to evaluate how deprivation of maternal feces influenced piglet physiology, behavior, and performance. Eight litters were randomly assigned to one of two treatments. Control (CON) litters had access to maternal feces while deprived (DEP) litters were deprived of maternal feces for the first 7 d post-partum. Piglet behavior was quantified for 24 h at 7 d of age. Blood samples were collected from one male and female from each litter at 0, 7, and 21 d for hematological analyses, and post-weaning performance was assessed until 123 d post-weaning. No treatment effects were observed on piglet behavior. DEP piglets had 25% lower leukocyte counts (p < 0.01). Relative to DEP litters, CON litters had increased post-weaning feed intake (0.998 vs 0.901 kg/d; p = 0.02) and weight gain (0.536 vs 0.483 kg/d; p < 0.01). At 123 d post-weaning, CON pigs were 9.3 ± 2.3 kg heavier than treatment pigs (p < 0.01). These results suggest that access to maternal feces improves immunocompetence and growth performance. Further studies are needed to explore the physiological mechanisms through which maternal feces improve growth performance, including nutritional and microbial factors, or the presence of maternal semiochemicals.