Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Brain Res Mol Brain Res ; 139(2): 201-11, 2005 Oct 03.
Article in English | MEDLINE | ID: mdl-15953654

ABSTRACT

A dramatic example of neuronal and physiological plasticity in adult mammals occurs during the transition from a non-maternal to a maternal, lactating state. In this study, we compared gene expression within a large continuous region of the CNS involved in maternal behaviors (hypothalamus, preoptic regions, and nucleus accumbens) between lactating (L) (postpartum Day 7) and randomly cycling virgin (V) outbred mice. Using high-density oligonucleotide arrays representing 11,904 genes, two statistical algorithms were used to identify significant differences in gene expression: robust multiarray (P < 0.001) (n = 92 genes) and significance analysis of microarrays using a 10% false discover rate (n = 114 genes). 27 common genes were identified as significant using both techniques. A subset of genes (n = 5) were selected and examined by real-time PCR. Our findings were consistent with previous published work. For example, neuropeptide Y (NPY) and proenkephalin were elevated in L mice, whereas POMC was decreased. Increased levels of NPY Y2 receptor and polo-like kinase and decreased levels of endothelin receptor type b in L mice are examples of novel gene expression changes not previously identified. Expression differences occurred in broad classes. Together, our findings provide possible new material on gene expression changes that may support maternal behaviors. The advantages and drawbacks of sampling large CNS regions using arrays are discussed.


Subject(s)
Central Nervous System/metabolism , Gene Expression Profiling , Gene Expression/physiology , Lactation/physiology , Maternal Behavior/physiology , Animals , Central Nervous System/anatomy & histology , Female , Male , Mice , Mice, Inbred ICR , Neuropeptide Y/genetics , Neuropeptide Y/metabolism , Pro-Opiomelanocortin/genetics , Pro-Opiomelanocortin/metabolism
2.
Proc Natl Acad Sci U S A ; 99(14): 9562-7, 2002 Jul 09.
Article in English | MEDLINE | ID: mdl-12089325

ABSTRACT

We measured daily gene expression in heads of control and period mutant Drosophila by using oligonucleotide microarrays. In control flies, 72 genes showed diurnal rhythms in light-dark cycles; 22 of these also oscillated in free-running conditions. The period gene significantly influenced the expression levels of over 600 nonoscillating transcripts. Expression levels of several hundred genes also differed significantly between control flies kept in light-dark versus constant darkness but differed minimally between per(01) flies kept in the same two conditions. Thus, the period-dependent circadian clock regulates only a limited set of rhythmically expressed transcripts. Unexpectedly, period regulates basal and light-regulated gene expression to a very broad extent.


Subject(s)
Circadian Rhythm/genetics , Circadian Rhythm/physiology , Drosophila melanogaster/genetics , Drosophila melanogaster/physiology , Genes, Insect , Nuclear Proteins/genetics , Nuclear Proteins/physiology , Animals , Drosophila Proteins , Drosophila melanogaster/radiation effects , Gene Expression/radiation effects , Gene Expression Profiling , Genes, Insect/radiation effects , Mutation , Oligonucleotide Array Sequence Analysis , Period Circadian Proteins , Photoperiod
SELECTION OF CITATIONS
SEARCH DETAIL
...