Ex vivo comparison of antibacterial efficacy of conventional chemomechanical debridement alone and in combination with light-activated disinfection and laser irradiation against Enterococcus faecalis biofilm.

BACKGROUND AND OBJECTIVES: Intracanal disinfection plays an important role in endodontic treatment success. Enterococcus faecalis (E. faecalis) is a resistant microorganism responsible for endodontic infections. We aimed to assess the bactericidal effects of three disinfection methods on E. faecalis biofilm. MATERIALS AND METHODS: Fifty-five freshly extracted single-rooted human teeth were evaluated. A barbed broach was used to extract the pulp tissue. No further root canal preparation was performed. Specimens were sterilized with gamma radiation, and inoculated with E. faecalis suspension. They were then incubated for 4 days and 4 weeks. Biofilm formation was confirmed using a scanning electron microscope (SEM). The teeth were randomly assigned to three subgroups (n = 7) to assess the antimicrobial efficacy of the following three disinfection methods against immature (4-day) and mature (4-week) biofilms: the conventional chemomechanical debridement (CCMD), CCMD + light-activated disinfection (LAD; 810 nm, 0.3 W, 120 J/cm2) with indocyanine Green (EmunDo) as photosensitizer and CCMD + diode laser irradiation (810 nm, 2 W). The teeth were then longitudinally split into two halves and the colony count was reported as colony forming units (CFUs) to assess bacterial viability after each disinfection protocol. RESULTS: None of the disinfection methods could completely remove the biofilm. CCMD + LAD caused the highest and CCMD + diode laser caused the lowest reduction in biofilm. Antibacterial efficacy was significantly lower against the mature (4-week) biofilm compared with immature (4-day) biofilm in all groups (P < 0.05). CONCLUSION: All three disinfection methods were effective for partial elimination of E. faecalis biofilm. But CCMD + LAD was significantly more efficacious in decreasing both mature and immature biofilms.

Evaluation of the antibacterial efficacy of various root canal disinfection methods against Enterococcus faecalis biofilm. An ex-vivo study.

BACKGROUND: Complete elimination of bacteria and their by-products from the root canal system is very difficult with current techniques. The purpose of this study was to compare the antibacterial efficacy of different disinfection protocols against Enterococcus faecalis (E. faecalis) biofilms. METHODS: Seventy-six extracted single-rooted human teeth were selected. Root canal preparation was done by proTaper rotary instruments. The smear layer was removed by 17% EDTA, followed by 5.25% sodium hypochlorite. After sterilization using gamma irradiation, sterilized specimens were inoculated with an E. faecalis suspension, incubated for 4 days and 4 weeks and then randomly divided into two experimental groups (4 days, 4 weeks old biofilms). After the confirmation of biofilm formation with SEM, the specimens in the experimental groups were randomly divided into five experimental subgroups according to the method of disinfection applied, which included: Diode laser irradiation (810 nm, 2 W), Light activated disinfection (LAD) with Indocyanine Green, 0.2% Chlorhexidine gluconate (0.2% CHX), 0.2% CHX + LAD and 0.2% CHX + Diode groups. RESULTS: Complete biofilm bacterial elimination was not observed in either of the experimental groups. CHX + LAD (0.2%) method exhibited the highest reduction value in biofilm and only Diode alone revealed the lowest in all the root canal portions. Disinfection protocols also showed significantly lower antibacterial efficacy against 4-week old than the 4-day old matured biofilms (P < 0.05). CONCLUSION: All the evaluated methods in this study were effective in the relative elimination of the E. faecalis biofilms except diode laser alone. Nevertheless, 0.2% CHX + LAD exhibited significantly higher efficacy in reducing both 4-day and 4-week old biofilms.