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Mol Biochem Parasitol ; 214: 101-104, 2017 06.
Article in English | MEDLINE | ID: mdl-28438458

ABSTRACT

In Trypanosoma brucei, the tetracycline-inducible system enables tightly-regulated, highly-efficient expression of recombinant proteins or double-stranded RNA in both procyclic and bloodstream form cells, providing useful molecular genetic tools to study gene functions. An alternative, vanillic acid-inducible system is recently described for procyclic T. brucei, providing ∼18-fold increase in GFP reporter expression upon induction (Sunter JD. Mol. Biochem. Parasitol. 2016, 207:45-48). Here we describe a cumate-inducible system that allows efficient, tunable gene expression showing >300-fold increase in GFP expression upon induction. The cumate-inducible system can be used alone or together with the tetracycline-inducible system, in both procyclic and bloodstream form T. brucei. Efficient cumate-inducible expression is also achieved in T. brucei-infected mice.


Subject(s)
Benzoates/metabolism , Molecular Biology/methods , Parasitology/methods , Transcriptional Activation/drug effects , Trypanosoma brucei brucei/drug effects , Trypanosoma brucei brucei/genetics
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