Correlative analysis of neoplasm patients with phlegm-stasis or abnormal Savda syndrome, based on metabonomics.

OBJECTIVE: To investigate plasma samples from neoplasm patients with phlegm-stasis or abnormal Savda syndrome, with NMR spectroscopy, and to analyze their metabolic varieties, characteristics and reciprocity. METHODS: 1H-NMR spectra were analyzed using the orthogonal projection to latent structure with discriminative analysis (OPLS-DA) method with unit variance scaling. The discriminative significance of metabolites was determined by the Pearson's product - moment correlation coefficient. RESULTS: Compared to the control group, neoplasm patients with phlegm - stasis or abnormal Savda syndrome had low concentrations of leucine, isoleucine, valine, alanine, tyrosine, histidine, citrulline, glycoprotein, glutamine, myo-inositol, scyllo-inositol, creatine, alpha-glucose, alpha-glucose and lactate (P < 0.05), and high concentrations of very low density lipoprotein, low density lipoprotein, unsaturated lipid, formate, acetone, acetate, acetoacetate, pyruvate, beta-hydroxybutyrate, carnitine and malonic acid (P < 0.05), with no significant differences between the phlegm - stasis and abnormal Savda syndrome patients. CONCLUSIONS: Neoplasm patients with different syndromes have very similar metabolic changes. A series of abnormalities such as immune dysfunction and oxidative - antioxidative imbalance, occur in neoplasm patients with abnormal Savda or phlegm - stasis syndrome.

Plasma metabonomic analysis with ¹H nuclear magnetic resonance revealing the relationship of different tumors and the disease homology theory of traditional Uyghur medicine / 中国结合医学杂志

<p><b>OBJECTIVE</b>To investigate the plasma samples obtained from tumor patients using (1)H nuclear magnetic resonance (NMR) spectroscopy, and find the biochemical foundation of abnormal Savda described in traditional Uyghur medicine.</p><p><b>METHODS</b>A total of 170 tumor patients with abnormal Savda syndrome who were confirmed clinically were enrolled in this study, and 50 healthy volunteers were set up as controls. The plasma (1)H NMR spectra were analyzed using the orthogonal projection to latent structure with discriminant analysis (OPLS-DA) method with unit variance scaling. The discriminative significance of the metabolites was determined using the Pearson's product-moment correlation coefficient.</p><p><b>RESULTS</b>Compared with the healthy controls, the tumor patients with abnormal Savda syndrome had uniformly correlative low levels of leucine, isoleucine, valine, histidine, tyrosine, alanine, glutamine, creatine, inositol, α-glucose, and β-glucose (P<0.05), but had significantly high levels of formate, malonic acid, acetone, acetate, acetoacetate, pyruvate, β-hydroxy butyrate, carnitine and lipidtemns such as very low density lipoprotein, low density lipoprotein and unsaturated lipids (P<0.05).</p><p><b>CONCLUSION</b>Tumor patients with abnormal Savda syndrome had similar metabolic changes and characteristics, which indicated a similar pathogenetic process and provides some biochemical basis for traditional Uyghur medicine theory.</p>