ABSTRACT
BACKGROUND: Aging decreases bone marrow cellularity and alters the frequencies of stem cells. Aged hematopoietic stem cells can differ from their younger counterparts in functional capacity. STUDY DESIGN AND METHODS: We aimed to evaluate the relation between the age and the ability of colony forming capacity of peripheral blood-derived hematopoietic cell products collected for autologous stem cell transplantation (AHSCT). RESULTS: Elderly patients could be mobilized with lower total collected CD34+ cells. Colony forming capacity did not differ between young and old patients. CONCLUSION: This results can be translated into clinic as higher numbers of AHSCT candidates over age 60.
Subject(s)
Aging , Hematopoietic Stem Cell Mobilization , Hematopoietic Stem Cells , Peripheral Blood Stem Cell Transplantation , Adolescent , Adult , Age Factors , Aged , Child , Child, Preschool , Female , Humans , Leukocyte Count , Male , Middle Aged , Neoplasms/blood , Neoplasms/therapy , Transplantation, AutologousABSTRACT
BACKGROUND: The viability of the hematopoietic stem cells infused to the patient is important for transplant outcome. STUDY DESIGN AND METHODS: We evaluated 31 peripheral blood stem cell product collected from 15 patients. We aimed to check the viabilities of the cells from patients with different age and diagnosis, in different stages of the cryopreservation procedure. RESULTS: We showed a markedly decreased viability rate after centrifugation and addition of DMSO. Percentages of viabilities were similar between young and old patients in each step. Type of hematological malignancy did not make a significant influence on the viability. CONCLUSION: High speed centrifugation has a negative impact on the viability.
Subject(s)
Cryopreservation , Hematologic Neoplasms/diagnosis , Hematologic Neoplasms/therapy , Hematopoietic Stem Cells , Peripheral Blood Stem Cell Transplantation , Adult , Age Factors , Aged , Cell Survival , Centrifugation , Female , Hematologic Neoplasms/blood , Humans , Male , Middle Aged , Transplantation, AutologousABSTRACT
Considerable information is available regarding the experimental and clinical applications of bone marrow derived stem cells (BMDSC) for regenerative medicine. Optimized stem cell delivery systems might help to maintain better stem cell viability. We have investigated whether needle diameters and flow rates through the needles cause any difference in terms of BMDSC viability.
Subject(s)
Bone Marrow Cells/cytology , Needles , Stem Cell Transplantation/instrumentation , Stem Cells/cytology , Adult , Aged , Cell Survival , Female , Flow Cytometry , Humans , Leukapheresis , Leukocytes, Mononuclear/cytology , Male , Middle Aged , Stem Cell Transplantation/methodsABSTRACT
Sn/PANI/p-Si/Al heterojunctions were fabricated by electropolymerization of aniline on chemically cleaned p-Si substrates. Current-voltage characteristics of Sn/PANI/p-Si/Al heterojunctions measured in the temperature range 140-280 K are presented and analyzed. Although these devices were clearly rectifying, their I-V characteristics were non-ideal, which can be judged from the nonlinearity in the semi-logarithmic plots. The high values of the ideality factor n depending on the sample temperature may be ascribed to a decrease of the exponentially increasing rate in current due to space-charge injection into the PANI thin film at higher forward bias voltages. Careful analysis of the forward bias I-V characteristics on a log-log scale indicates that the space-charge-limited current (SCLC) conduction controlled by an exponential trap distribution above the valence band edge dominates the current transport in the PANI/p-Si diodes at high voltages. Furthermore, the PANI was characterized by using Fourier transform infrared (FTIR) and ultraviolet-visible (UV-vis) spectra.
