ABSTRACT
BACKGROUND: The permeability feature of conventional calcium hydroxide (CH) and nanoparticle CH (NCH) was compared to show the desired effects of this new material and in case of confirmation of its other properties; CH can be used as a safe alternative. MATERIALS AND METHODS: This in vitro was carried out in two phases: First phase: measurement and comparison of the permeability rate of conventional CH and NCH in the dentinal tubules employing a fluorescence microscope were carried out. Second phase: measurement and comparison of the permeability rate of NCH and conventional CH in L929 fibroblast cells using a fluorescence microscope were carried out. Kruskal-Wallis analysis was used for overall comparisons. A series of Mann-Whitney U tests were used for pair-wise comparisons (P < 0.05). RESULTS: Based on the results of Kruskal-Wallis test, in all three regions of the cervical third, middle third, and apical third of the root, mean values of the percentage difference of the fluorescence and color change in NCH were more than the conventional CH, and the difference was statistically significant (P < 0.001). the percentage of fluorescence color change in drug with the concentration of 1 g/cc was more than the one with the concentration of 0.1 g/cc and the difference was statistically significant (P < 0.001). CONCLUSION: The nanoparticle drug compared with the conventional drug has a more penetration depth in all regions of the root of the dentinal tubules.
ABSTRACT
INTRODUCTION: One of the major contributing factors, which may cause failure of endodontic treatment, is the presence of residual microorganisms in the root canal system. For years, most dentists have been using calcium hydroxide (CH) as the intracanal medicament between treatment sessions to eliminate remnant microorganisms. Reducing the size of CH particles into nanoparticles enhances the penetration of this medicament into dentinal tubules and increases their antimicrobial efficacy. This in vitro study aimed to compare the cytotoxicity of CH nanoparticles and conventional CH on fibroblast cell line using the Mosmann's Tetrazolium Toxicity (MTT) assay. METHODS AND MATERIALS: This study was conducted on L929 murine fibroblast cell line by cell culture and evaluation of the direct effect of materials on the cultured cells. Materials were evaluated in two groups of 10 samples each at 24, 48 and 72 h. At each time point, 10 samples along with 5 positive and 5 negative controls were evaluated. The samples were transferred into tubes and exposed to fibroblast cells. The viability of cells was then evaluated. The Two-way ANOVA was used for statistical analysis and the level of significance was set at 0.05. RESULTS: Cytotoxicity of both materials decreased over time and for conventional CH was lower than that of nanoparticles. However, this difference was not statistically significant (P>0.05). CONCLUSION: The cytotoxicity of CH nanoparticles was similar to that of conventional CH.
ABSTRACT
A healthy female was referred to Endodontic Department. The referral letter from her dentist expressed that an emergency pulpotomy of tooth #27 had been carried out with probable perforation of the chamber floor which was due to the unusual anatomy of the chamber. Cone-beam computed tomography (CBCT) revealed that the tooth had two mesial and two distal canals. Perforation site was repaired and endodontic treatment was completed. At 24-month follow-up, patient was asymptomatic and clinical and radiographic examinations showed successful outcomes.
