Cultivation of two Chlorella species in Open sewage contaminated channel wastewater for biomass and biochemical profiles: Comparative lab-scale approach.

Open sewage contaminated channel wastewater (OSCCW) has high pollutant loads, responsible for eutrophication, when mixed with various channels of urban communities. But, these pollutants can be converted and recovered into useful end products with the help of algal species. In this study, two species of Chlorella (C. vulgaris and C. pyrenoidosa) were selected and investigated for the production of algal biomass and nutrient removal efficiencies with 50% concentration of OSCCW, in a comparative way at lab-scale. Chlorella sp. cultivated in OSCCW have removed nitrate (76.9-78.8%) and phosphate (67.6-79.7%) whereas COD (72.4-76.2%) and BOD (62.3-72.4%) respectively. Correlation analysis was investigated between physico-chemical parameters and biochemical profile of both species to analyze the positive and negative correlation between two variables. The bio-chemical profile and biomass productivity of both species of Chlorella were observed well on the basis of productivity of biomass (60.1, 56.5 mg/l/d), carbohydrate (15.71, 8.82 mg/l/d), protein (11.21, 15.82 mg/l/d), lipid (20.8, 17.5 mg/l/d) and chlorophyll (0.78, 0.67 mg/l/d) in OSCCW. The maximum lipid content (34.6%) was obtained with C. pyrenoidosa as compared to C. vulgaris. Findings also support that OSCCW is well-off with nutrient resources, which can be suitable alternative for algal biomass production and remediated wastewater can be used for animal and fish farming type activities.

Novel approach for harvesting of microalgal biomass using electric geyser waste material deposit as flocculant in coupling with poultry excreta leachate.

The aim of this work was to study the flocculation efficiency of algal biomass (Chlorella pyrenoidosa) in coupling with waste materials i.e. poultry excreta leachate by using other waste material which was obtained from deposition of scaling in electric geyser. Utilization of electric geyser waste material deposit (EGWMD) for flocculation is a novel approach because of various elements which are replica of chemical flocculants responsible for flocculation mechanism in culture medium. Flocculation process was optimized by response surface methodology and 98.21% flocculation efficiency was achieved with designed process parameters as temperature 32.5 °C, flocculant dose 275 mgL-1, pH 5 and time 30 min. The reusability of spent medium was also analyzed at 70.2% and 32.5% flocculation efficiency with two successive steps. The cellular morphology of pre-harvested and post-harvested Chlorella pyrenoidosa was also observed. EGWMD is abundant and freely available that has no application till now and can alternate of chemical flocculants.

Bioprocessing of cultivated Chlorella pyrenoidosa on poultry excreta leachate to enhance algal biomolecule profile for resource recovery.

The aim of this work was to study the cultivation of Chlorella pyrenoidosa on poultry excreta leachate to enhance the biochemical composition of algal biomass. The growth of microalgae was analyzed with different concentrations of poultry excreta leachate in BG-11 and distilled water. The biomolecules observed have high value in the form of carbohydrates (0.64 gL-1), protein (1.02 gL-1), chlorophyll (20 µg mL-1) and lipid amount (0.49 gL-1) with PEL BG -25%. Biomass produced in PEL BG -25% was also found to be 60% (2.5 gL-1) higher than the BG-11 medium as a control (1.5gL-1). Recovery of nutrients was observed with leachate wastewater concentration in terms of nitrate (84.2%), ammonium nitrogen (53.1%), and inorganic phosphate (96.2%). Hence, sustainability of microalgae cultivation in wastewater provides a new insight for resource utilization.

Production of algal biomass for its biochemical profile using slaughterhouse wastewater for treatment under axenic conditions.

Slaughterhouse produce large amount of wastewater, containing high pollutant load in terms of protein, fats and meat pieces, might lead to source of non-point contamination. Various concentrations (25%, 50%, 75%, and 100%) of slaughterhouse wastewater were used to increase the algal biomass production, pollutants removal and biochemical profile analysis under controlled conditions of C. pyrenoidosa. Results showed that the maximum biomass yield 430 mg L-1 was achieved at 50% concentration of wastewater to other concentration of wastewater. Direct relation was observed in between pollution load and nutrient load of SHWW with biochemical profile of C. pyrenoidosa. The COD/BOD ratio (1.9) was found to be significant on the scale of degradability by algal biomass. Sufficient nutrient removal efficiencies (23-42%, 18-48%) and pollutant load efficiencies (17-31%, 7-29%) were observed. Findings showed that slaughterhouse wastewater is rich in nutrients, which can be utilized for algal biomass production and wastewater remediation for future endeavors.