ABSTRACT
The effect of serum of patients with myocardial ischemia after low-level laser therapy on parameters of synaptic conductance of rat hippocampal neurons was investigated. The serum from patients with an initially low level of neuronal activity obtained after determination of laser irradiation increased the amplitude and that from patients with high activity. Thus the process of normalization of these parameters was observed. Our results may help to optimize the course of medical treatment, and subsequently give an insight to understanding of the mechanism of therapeutic effect of laser irradiation.
Subject(s)
Blood/radiation effects , Laser Therapy , Synapses/drug effects , Action Potentials/drug effects , Action Potentials/physiology , Adult , Animals , Electric Stimulation , Hippocampus/drug effects , Hippocampus/physiology , Humans , In Vitro Techniques , Middle Aged , Myocardial Ischemia/blood , Myocardial Ischemia/radiotherapy , Neurons/drug effects , Neurons/physiology , Rats , Rats, Wistar , Reaction Time/drug effects , Reaction Time/physiology , Synapses/physiology , Synaptic Transmission/drug effects , Synaptic Transmission/physiologyABSTRACT
The isolated neurons of snail (Lymnaea stagnalis) were as test object for the evaluation of the blood serum neuroactivity. The blood serum was tested before treatment and after six applications of intravenous laser therapy (ILT). All blood serum specimens produced depolarization of the cell membrane potential. The quantitative comparison of membrane toxicity of control and experimental blood serum specimens permitted to divide the patients into three groups. Blood serum (after six applications of ILT of patients from the first and second groups produced a lower membrane toxicity effect on neurons. In the groups the membrane toxicity effect decrease by 43% +/- 10.3 and 7.3 +/- 2.2% respectively. In third group the membrane toxicity effect increased by 21.5 +/- 4.5. All patients from the third group had myocardial infarction less than one year before being given a course on the treatment. The ILT was found to be able to decrease the activity of the blood serum components disrupting normal functioning of the ionic mechanisms of cell membranes.
Subject(s)
Myocardial Ischemia/blood , Neurons/drug effects , Animals , Blood/radiation effects , Humans , Laser Therapy , Lymnaea , Membrane Potentials/drug effects , Membrane Potentials/physiology , Myocardial Ischemia/radiotherapy , Neurons/physiologyABSTRACT
Influence of UV irradiation (254 nm) on chemoluminescence of blood granulocytes was studied in patients with chronic unspecific lung impairments. Chemoluminescence of leukocytes was decreased after blood irradiation at the rate 10 J/ml as well as after the first course of the UV irradiated blood transfusion. At the same time, high dissimilarity of reactions to the irradiation was found in individual patients: in patients with low initial parameters of metabolism a stable decrease of chemoluminescence was observed to the 2nd, 3d and 4th courses of the irradiation, while in patients with high parameters--only to the 2nd course. This suggests that functional activity of phagocytes may be altered due to UV irradiation effects.
